学術雑誌論文

学術雑誌論文

text

Scientific Reports. 2017, 7(1), p. 7335

Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Nature Research

英語

text

2017-08-04

https://doi.org/10.1038/s41598-017-07777-6

Phengchat, R., Takata, H., Uchiyama, S. et al. Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore. Sci Rep 7, 7335 (2017). https://doi.org/10.1038/s41598-017-07777-6.

The attachment of spindle fibres to the kinetochore is an important process that ensures successful completion of the cell division. The Ca²⁺ concentration increases during the mitotic phase and contributes microtubule stability. However, its role in the spindle organisation in mitotic cells remains controversial. Here, we investigated the role of Ca²⁺ on kinetochore fibres in living cells. We found that depletion of Ca²⁺ during mitosis reduced kinetochore fibre stability. Reduction of kinetochore fibre stability was not due to direct inhibition of microtubule polymerisation by Ca2⁺- depletion but due to elimination of one dynamic component of kinetochore, CENP-F from the kinetochore. This compromised the attachment of kinetochore fibres to the kinetochore which possibly causes mitotic defects induced by the depletion of Ca²⁺.

© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.

79024

Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Nature Research

英語

2017-08-04

Scientific Reports

7

1

7335

https://doi.org/10.1038/s41598-017-07777-6

28779172

https://hdl.handle.net/11094/79024