学術雑誌論文

学術雑誌論文

text

Scientific Reports. 2015, 5, p. 11916

Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins

Nature Research

英語

text

2015-07-01

https://doi.org/10.1038/srep11916

Poonperm, R., Takata, H., Hamano, T. et al. Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins. Sci Rep 5, 11916 (2015). https://doi.org/10.1038/srep11916.

Chromosome higher order structure has been an enigma for over a century. The most important structural finding has been the presence of a chromosome scaffold composed of non-histone proteins; so-called scaffold proteins. However, the organization and function of the scaffold are still controversial. Here, we use three dimensional-structured illumination microscopy (3D-SIM) and focused ion beam/scanning electron microscopy (FIB/SEM) to reveal the axial distributions of scaffold proteins in metaphase chromosomes comprising two strands. We also find that scaffold protein can adaptably recover its original localization after chromosome reversion in the presence of cations. This reversion to the original morphology underscores the role of the scaffold for intrinsic structural integrity of chromosomes. We therefore propose a new structural model of the chromosome scaffold that includes twisted double strands, consistent with the physical properties of chromosomal bending flexibility and rigidity. Our model provides new insights into chromosome higher order structure.

© 2015 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License.

79031

Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins

Nature Research

英語

2015-07-01

Scientific Reports

5

11916

https://doi.org/10.1038/srep11916

26132639

https://hdl.handle.net/11094/79031