Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells

Amin, Mohammed Abdullahel; Matsunaga, Sachihiro; Uchiyama, Susumu; Fukui, Kiichi

doi:https://doi.org/10.1042/BJ20081411

Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells

Amin, Mohammed Abdullahel; Matsunaga, Sachihiro; Uchiyama, Susumu et al.

Biochemical Journal, 2008, 415(3), 345-351

アクセス数:521件（2025-07-27 09:28 集計）

固定URL: https://hdl.handle.net/11094/79045

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タイトル	Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
著者	著者名 Amin, Mohammed Abdullahel
	NRID 1000040323448 1000040323448 著者名 Matsunaga, Sachihiro
	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 著者名 Uchiyama, Susumu
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 著者名 Fukui, Kiichi
キーワード等	HeLa cell
	Nuclear structure
	Nucleolar structure
	Nucleophosmin (NPM)
	RNA interference (RNAi)
内容	Mohammed Abdullahel Amin, Sachihiro Matsunaga, Susumu Uchiyama, Kiichi Fukui; Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells. Biochem J 1 November 2008; 415 (3): 345–351. doi: https://doi.org/10.1042/BJ20081411.
抄録	NPM (nucleophosmin; also known as B23) is an abundantly and ubiquitously expressed multifunctional nucleolar phosphoprotein, which is involved in numerous cellular processes, including ribosome biogenesis, protein chaperoning and centrosome duplication; however, the role of NPM in the cell cycle still remains unknown. In the present study, we show dynamic localization of NPM throughout the cell cycle of HeLa cells. Using a combination of RNAi (RNA interference) and three-dimensional microscopy we show that NPM is localized at the chromosome periphery during mitosis. We also demonstrate that depletion of NPM causes distortion of nucleolar structure as expected and leads to unexpected dramatic changes in nuclear morphology with multiple micronuclei formation. The defect in nuclear shape of NPM-depleted cells, which is clearly observed by live-cell imaging, is due to the distortion of cytoskeletal (α-tubulin and β-actin) structure, resulting from the defects in centrosomal microtubule nucleation. These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells.
出版者	Biochemical Society
収録物名	Biochemical Journal
巻(号)	415(3)
ページ	345-351
刊行年月	2008-10-15
言語	英語
ハンドルURL	https://hdl.handle.net/11094/79045
PISSN	02646021
eISSN	14708728
NCID	AA10649144
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1042/BJ20081411
関連情報 (isIdenticalTo)	PMID 18729828
アクセス権	open access
権利情報	© 2008 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial 2.5 Generic License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biochemical Journal. 2008, 415(3), p. 345-351
DC.title	Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
DC.creator	Amin, Mohammed Abdullahel
	Matsunaga, Sachihiro
	Uchiyama, Susumu
	Fukui, Kiichi
DC.publisher	Biochemical Society
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2008-10-15
DC.identifier	https://doi.org/10.1042/BJ20081411
DC.subject	HeLa cell
	Nuclear structure
	Nucleolar structure
	Nucleophosmin (NPM)
	RNA interference (RNAi)
DC.description	Mohammed Abdullahel Amin, Sachihiro Matsunaga, Susumu Uchiyama, Kiichi Fukui; Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells. Biochem J 1 November 2008; 415 (3): 345–351. doi: https://doi.org/10.1042/BJ20081411.
DCTERMS.abstract	NPM (nucleophosmin; also known as B23) is an abundantly and ubiquitously expressed multifunctional nucleolar phosphoprotein, which is involved in numerous cellular processes, including ribosome biogenesis, protein chaperoning and centrosome duplication; however, the role of NPM in the cell cycle still remains unknown. In the present study, we show dynamic localization of NPM throughout the cell cycle of HeLa cells. Using a combination of RNAi (RNA interference) and three-dimensional microscopy we show that NPM is localized at the chromosome periphery during mitosis. We also demonstrate that depletion of NPM causes distortion of nucleolar structure as expected and leads to unexpected dramatic changes in nuclear morphology with multiple micronuclei formation. The defect in nuclear shape of NPM-depleted cells, which is clearly observed by live-cell imaging, is due to the distortion of cytoskeletal (α-tubulin and β-actin) structure, resulting from the defects in centrosomal microtubule nucleation. These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells.
DC.rights	© 2008 The Author(s). This article is licensed under a Creative Commons Attribution-NonCommercial 2.5 Generic License.
DC.format	79045
citation_title	Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells
citation_author	Amin, Mohammed Abdullahel
	Matsunaga, Sachihiro
	Uchiyama, Susumu
	Fukui, Kiichi
citation_publisher	Biochemical Society
citation_language	英語
citation_date	2008-10-15
citation_journal_title	Biochemical Journal
citation_volume	415
citation_issue	3
citation_firstpage	345
citation_lastpage	351
citation_doi	https://doi.org/10.1042/BJ20081411
citation_pmid	18729828
citation_public_url	https://hdl.handle.net/11094/79045

Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells

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