Proteome analysis of human metaphase chromosomes

Uchiyama, Susumu; Kobayashi, Shouhei; Takata, Hideaki; Ishihara, Takeshi; Hori, Naoto; Higashi, Tsunehito; Hayashihara, Kayoko; Sone, Takefumi; Higo, Daisuke; Nirasawa, Takashi; Takao, Toshifumi; Matsunaga, Sachihiro; Fukui, Kiichi

doi:https://doi.org/10.1074/jbc.M412774200

Proteome analysis of human metaphase chromosomes

Uchiyama, Susumu; Kobayashi, Shouhei; Takata, Hideaki et al.

Journal of Biological Chemistry, 2005, 280(17), 16994-17004

アクセス数:1,507件（2025-07-27 09:28 集計）

固定URL: https://hdl.handle.net/11094/79049

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
JBiolChem_280_17_16994	pdf	なし	2.20 MB	222	2021.03.16

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Proteome analysis of human metaphase chromosomes
著者	NRID 1000090335381 1000090335381 ORCID 0000-0002-5181-179X 0000-0002-5181-179X researchmap permalink read0090387 read0090387 Scopus Author ID 35429790100 35429790100 著者名 Uchiyama, Susumu
	NRID 1000040425765 1000040425765 著者名 Kobayashi, Shouhei
	NRID 1000020455207 1000020455207 著者名 Takata, Hideaki
	著者名 Ishihara, Takeshi
	著者名 Hori, Naoto
	NRID 1000090453018 1000090453018 著者名 Higashi, Tsunehito
	NRID 1000000624389 1000000624389 著者名 Hayashihara, Kayoko
	著者名 Sone, Takefumi
	著者名 Higo, Daisuke
	著者名 Nirasawa, Takashi
	NRID 1000010197048 1000010197048 researchmap permalink Toshifumi_Takao Toshifumi_Takao Scopus Author ID 7102574661 7102574661 著者名 Takao, Toshifumi
	NRID 1000040323448 1000040323448 著者名 Matsunaga, Sachihiro
	NRID 1000000311770 1000000311770 researchmap permalink read0068600 read0068600 著者名 Fukui, Kiichi
内容	Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Takeshi Ishihara, Naoto Hori, Tsunehito Higashi, Kayoko Hayashihara, Takefumi Sone, Daisuke Higo, Takashi Nirasawa, Toshifumi Takao, Sachihiro Matsunaga, Kiichi Fukui. Proteome Analysis of Human Metaphase Chromosomes. Journal of Biological Chemistry, Volume 280, Issue 17, 2005, Pages 16994-17004. https://doi.org/10.1074/jbc.M412774200.
抄録	DNA is packaged as chromatin in the interphase nucleus. Dining mitosis, chromatin fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal DNA into the daughter cells. Despite > 1 century of research on metaphase chromosomes, information regarding the higher order structure of metaphase chromosomes is limited, and it is still not clear which proteins are involved in further folding of the chromatin fiber into metaphase chromosomes. To obtain a global view of the chromosomal proteins, we performed proteome analyses on three types of isolated human metaphase chromosomes. We first show the results from comparative proteome analyses of two types of isolated human metaphase chromosomes that have been frequently used in biochemical and morphological analyses. 209 proteins were quantitatively identified and classified into sis groups on the basis of their known interphase localization. Furthermore, a list of 107 proteins was obtained from the proteome analyses of highly purified metaphase chromosomes, the majority of which are essential for chromosome structure and function. Based on the information obtained on these proteins and on their localizations during mitosis as assessed by immunostaining, we present a four-layer model of metaphase chromosomes. According to this model, the chromosomal proteins have been newly classified into each of four groups: chromosome coating proteins, chromosome peripheral proteins, chromosome structural proteins, and chromosome fibrous proteins. This analysis represents the first compositional view of human metaphase chromosomes and provides a protein frame-work for future research on this topic.
出版者	American Society for Biochemistry and Molecular Biology
収録物名	Journal of Biological Chemistry
巻(号)	280(17)
ページ	16994-17004
刊行年月	2005-04-29
言語	英語
ハンドルURL	https://hdl.handle.net/11094/79049
PISSN	00219258
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1074/jbc.M412774200
関連情報 (isIdenticalTo)	PMID 15687487
アクセス権	open access
権利情報	© 2005 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Biological Chemistry. 2005, 280(17), p. 16994-17004
DC.title	Proteome analysis of human metaphase chromosomes
DC.creator	Uchiyama, Susumu
	Kobayashi, Shouhei
	Takata, Hideaki
	Ishihara, Takeshi
	Hori, Naoto
	Higashi, Tsunehito
	Hayashihara, Kayoko
	Sone, Takefumi
	Higo, Daisuke
	Nirasawa, Takashi
	Takao, Toshifumi
	Matsunaga, Sachihiro
	Fukui, Kiichi
DC.publisher	American Society for Biochemistry and Molecular Biology
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2005-04-29
DC.identifier	https://doi.org/10.1074/jbc.M412774200
DC.description	Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Takeshi Ishihara, Naoto Hori, Tsunehito Higashi, Kayoko Hayashihara, Takefumi Sone, Daisuke Higo, Takashi Nirasawa, Toshifumi Takao, Sachihiro Matsunaga, Kiichi Fukui. Proteome Analysis of Human Metaphase Chromosomes. Journal of Biological Chemistry, Volume 280, Issue 17, 2005, Pages 16994-17004. https://doi.org/10.1074/jbc.M412774200.
DCTERMS.abstract	DNA is packaged as chromatin in the interphase nucleus. Dining mitosis, chromatin fibers are highly condensed to form metaphase chromosomes, which ensure equal segregation of replicated chromosomal DNA into the daughter cells. Despite > 1 century of research on metaphase chromosomes, information regarding the higher order structure of metaphase chromosomes is limited, and it is still not clear which proteins are involved in further folding of the chromatin fiber into metaphase chromosomes. To obtain a global view of the chromosomal proteins, we performed proteome analyses on three types of isolated human metaphase chromosomes. We first show the results from comparative proteome analyses of two types of isolated human metaphase chromosomes that have been frequently used in biochemical and morphological analyses. 209 proteins were quantitatively identified and classified into sis groups on the basis of their known interphase localization. Furthermore, a list of 107 proteins was obtained from the proteome analyses of highly purified metaphase chromosomes, the majority of which are essential for chromosome structure and function. Based on the information obtained on these proteins and on their localizations during mitosis as assessed by immunostaining, we present a four-layer model of metaphase chromosomes. According to this model, the chromosomal proteins have been newly classified into each of four groups: chromosome coating proteins, chromosome peripheral proteins, chromosome structural proteins, and chromosome fibrous proteins. This analysis represents the first compositional view of human metaphase chromosomes and provides a protein frame-work for future research on this topic.
DC.rights	© 2005 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	79049
citation_title	Proteome analysis of human metaphase chromosomes
citation_author	Uchiyama, Susumu
	Kobayashi, Shouhei
	Takata, Hideaki
	Ishihara, Takeshi
	Hori, Naoto
	Higashi, Tsunehito
	Hayashihara, Kayoko
	Sone, Takefumi
	Higo, Daisuke
	Nirasawa, Takashi
	Takao, Toshifumi
	Matsunaga, Sachihiro
	Fukui, Kiichi
citation_publisher	American Society for Biochemistry and Molecular Biology
citation_language	英語
citation_date	2005-04-29
citation_journal_title	Journal of Biological Chemistry
citation_volume	280
citation_issue	17
citation_firstpage	16994
citation_lastpage	17004
citation_doi	https://doi.org/10.1074/jbc.M412774200
citation_pmid	15687487
citation_public_url	https://hdl.handle.net/11094/79049

Proteome analysis of human metaphase chromosomes

閲覧可能ファイル

論文情報

論文詳細を表示