Gelatin microspheres crosslinked with γ-ray : Preparation, sorption of proteins, and biodegradability

Terao, Ken; Karino, Tomotake; Nagasawa, Naotsugu; Yoshii, Fumio; Kubo, Miyoko; Dobashi, Toshiaki

doi:https://doi.org/10.1002/app.13574

Gelatin microspheres crosslinked with γ-ray : Preparation, sorption of proteins, and biodegradability

Terao, Ken; Karino, Tomotake; Nagasawa, Naotsugu et al.

Journal of Applied Polymer Science, 2004, 91(5), 3083-3087

アクセス数:568件（2025-10-26 01:03 集計）

固定URL: https://hdl.handle.net/11094/81796

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タイトル	Gelatin microspheres crosslinked with γ-ray : Preparation, sorption of proteins, and biodegradability
著者	NRID 1000060334132 1000060334132 ORCID 0000-0001-7363-4491 0000-0001-7363-4491 researchmap permalink kenterao kenterao Scopus Author ID 7101653015 7101653015 著者名 Terao, Ken
	著者名 Karino, Tomotake
	NRID 1000000370437 1000000370437 著者名 Nagasawa, Naotsugu
	著者名 Yoshii, Fumio
	NRID 1000000098609 1000000098609 著者名 Kubo, Miyoko
	NRID 1000030155626 1000030155626 著者名 Dobashi, Toshiaki
キーワード等	Gels
	Irradiation
	Crosslinking
	Biodegradable
	Proteins
内容	Terao, K., Karino, T., Nagasawa, N., Yoshii, F., Kubo, M. and Dobashi, T. (2004), Gelatin microspheres crosslinked with γ-ray: Preparation, sorption of proteins, and biodegradability. J. Appl. Polym. Sci., 91: 3083-3087. https://doi.org/10.1002/app.13574.
抄録	A new type of gelatin microspheres was manufactured with the crosslinking method by using γ-ray irradiation. Microspheres thus obtained were characterized by microscopic observation and enzymatic degradability. The mean diameter of the microspheres was in the range from 2.4 to 3.6 μm and the size distribution was quite small. The size decreased with increasing the shear rate of the emulsifier used in the preparation and was not affected by the radiation dose. The enzymatic degradability decreased with increasing the radiation dose and decreasing the gelatin concentration of the microsphere. In other words, the rate of the enzymatic decomposition of microspheres can be controlled by these two parameters. These trends were consistent with our previous results for the gel sheet made of gelatin. Their sorption of proteins was also investigated with the use of three types of proteins labeled by fluorescent pigment. From the fluorescence micrographs, it was observed that the gelatin microspheres adsorbed only proteins having the opposite charge.
出版者	Wiley
収録物名	Journal of Applied Polymer Science
巻(号)	91(5)
ページ	3083-3087
刊行年月	2004-03-05
言語	英語
ハンドルURL	https://hdl.handle.net/11094/81796
PISSN	00218995
NCID	AA00693605
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1002/app.13574
アクセス権	open access
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Applied Polymer Science. 2004, 91(5), p. 3083-3087
DC.title	Gelatin microspheres crosslinked with γ-ray : Preparation, sorption of proteins, and biodegradability
DC.creator	Terao, Ken
	Karino, Tomotake
	Nagasawa, Naotsugu
	Yoshii, Fumio
	Kubo, Miyoko
	Dobashi, Toshiaki
DC.publisher	Wiley
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2004-03-05
DC.identifier	https://doi.org/10.1002/app.13574
DC.subject	Gels
	Irradiation
	Crosslinking
	Biodegradable
	Proteins
DC.description	Terao, K., Karino, T., Nagasawa, N., Yoshii, F., Kubo, M. and Dobashi, T. (2004), Gelatin microspheres crosslinked with γ-ray: Preparation, sorption of proteins, and biodegradability. J. Appl. Polym. Sci., 91: 3083-3087. https://doi.org/10.1002/app.13574.
DCTERMS.abstract	A new type of gelatin microspheres was manufactured with the crosslinking method by using γ-ray irradiation. Microspheres thus obtained were characterized by microscopic observation and enzymatic degradability. The mean diameter of the microspheres was in the range from 2.4 to 3.6 μm and the size distribution was quite small. The size decreased with increasing the shear rate of the emulsifier used in the preparation and was not affected by the radiation dose. The enzymatic degradability decreased with increasing the radiation dose and decreasing the gelatin concentration of the microsphere. In other words, the rate of the enzymatic decomposition of microspheres can be controlled by these two parameters. These trends were consistent with our previous results for the gel sheet made of gelatin. Their sorption of proteins was also investigated with the use of three types of proteins labeled by fluorescent pigment. From the fluorescence micrographs, it was observed that the gelatin microspheres adsorbed only proteins having the opposite charge.
DC.format	81796
citation_title	Gelatin microspheres crosslinked with γ-ray : Preparation, sorption of proteins, and biodegradability
citation_author	Terao, Ken
	Karino, Tomotake
	Nagasawa, Naotsugu
	Yoshii, Fumio
	Kubo, Miyoko
	Dobashi, Toshiaki
citation_publisher	Wiley
citation_language	英語
citation_date	2004-03-05
citation_journal_title	Journal of Applied Polymer Science
citation_volume	91
citation_issue	5
citation_firstpage	3083
citation_lastpage	3087
citation_doi	https://doi.org/10.1002/app.13574
citation_public_url	https://hdl.handle.net/11094/81796

Gelatin microspheres crosslinked with γ-ray : Preparation, sorption of proteins, and biodegradability

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