学術雑誌論文

学術雑誌論文

text

Analytical Chemistry. 2008, 80(14), p. 5494-5500

Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance

American Chemical Society

英語

text

2008-07-01

https://doi.org/10.1021/ac800459g

Hirotsugu Ogi, Yuji Fukunishi, Toshinobu Omori, Kenichi Hatanaka, Masahiko Hirao, and Masayoshi Nishiyama. Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance. Analytical Chemistry, 2008, 80(14), 5494-5500. ©2008 American Chemical Society. https://doi.org/10.1021/ac800459g.

In this paper, we developed a 55-MHz wireless-electrodeless quartz crystal microbalance (QCM) and systematically studied the effects of flow rate on the sensitivity to the detection of proteins and on the affinity between biomolecules evaluated by the flow injection system. Brownian motion of proteins in liquid suggests a low probability of meeting, and the convection effect plays an important role in the sensitivity and the affinity in the flow cell injection system. The wireless quartz crystal was isolated in the QCM cell, and flow rates between 50 and 1000 μL/min were used for monitoring binding reactions between human immunoglobulin G and Staphylococcus aureus protein A. The sensitivity was significantly increased as the flow rate increased, while the affinity value remained unchanged. However, the affinity value was affected by the reaction time for a large-concentration analyte, indicating the need of a high-sensitivity biosensor system for accurate evaluation of affinity. The electrode effect on the QCM sensitivity was also theoretically investigated, showing that the electrode significantly deteriorates the QCM sensitivity and makes the Sauerbrey equation invalid.

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical Chemistry, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/ac800459g.

84138

Effects of flow rate on sensitivity and affinity in flow injection biosensor systems studied by 55-MHz wireless quartz crystal microbalance

American Chemical Society

英語

2008-07-01

Analytical Chemistry

80

14

5494

5500

https://doi.org/10.1021/ac800459g

18553940

https://hdl.handle.net/11094/84138