Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins

Ogi, Hirotsugu; Nagai, Hironao; Fukunishi, Yuji; Yanagida, Taiji; Hirao, Masahiko; Nishiyama, Masayoshi

doi:https://doi.org/10.1143/JJAP.49.07HD07

Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins

Ogi, Hirotsugu; Nagai, Hironao; Fukunishi, Yuji et al.

Japanese Journal of Applied Physics, 2010, 49, 07HD07

アクセス数:337件（2025-08-23 10:31 集計）

固定URL: https://hdl.handle.net/11094/84165

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タイトル	Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins
著者	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 著者名 Ogi, Hirotsugu
	著者名 Nagai, Hironao
	著者名 Fukunishi, Yuji
	著者名 Yanagida, Taiji
	NRID 1000080112027 1000080112027 著者名 Hirao, Masahiko
	著者名 Nishiyama, Masayoshi
内容	Hirotsugu Ogi, Hironao Nagai, Yuji Fukunishi, Taiji Yanagida, Masahiko Hirao and Masayoshi Nishiyama. Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins. Japanese Journal of Applied Physics, 2010, 49(7S), 07HD07. https://doi.org/10.1143/JJAP.49.07HD07.
抄録	Staphylococcus aureus protein A (SPA) shows high nonspecific binding affinity on a naked quartz surface, and it can be used as the receptor protein for detecting immunoglobulin G (IgG), the most important immunoglobulin. The immunosensor ability, however, significantly depends on the immobilization procedure. In this work, the effect of the nonspecific immobilization procedure on the sensor sensitivity is studied using a homebuilt electrodeless quartz-crystal microbalance (QCM) biosensor. The pure-shear vibration of a 9.7-mm-thick AT-cut quartz plate is excited and detected in liquids by the line antenna located outside the flow channel. SPA molecules are immobilized on the quartz surfaces, and human IgG is injected to monitor the binding reaction between SPA and IgG. This study reveals that a long (nearly 24 h) immersion procedure is required for immobilizing SPA to achieve the tight biding with the quartz surfaces.
出版者	The Japan Society of Applied Physics
収録物名	Japanese Journal of Applied Physics
巻(号)	49
ページ	07HD07
刊行年月	2010-07-20
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84165
PISSN	00214922
eISSN	13474065
NCID	AA12295836
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1143/JJAP.49.07HD07
アクセス権	open access
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Japanese Journal of Applied Physics. 2010, 49, p. 07HD07
DC.title	Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins
DC.creator	Ogi, Hirotsugu
	Nagai, Hironao
	Fukunishi, Yuji
	Yanagida, Taiji
	Hirao, Masahiko
	Nishiyama, Masayoshi
DC.publisher	The Japan Society of Applied Physics
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-07-20
DC.identifier	https://doi.org/10.1143/JJAP.49.07HD07
DC.description	Hirotsugu Ogi, Hironao Nagai, Yuji Fukunishi, Taiji Yanagida, Masahiko Hirao and Masayoshi Nishiyama. Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins. Japanese Journal of Applied Physics, 2010, 49(7S), 07HD07. https://doi.org/10.1143/JJAP.49.07HD07.
DCTERMS.abstract	Staphylococcus aureus protein A (SPA) shows high nonspecific binding affinity on a naked quartz surface, and it can be used as the receptor protein for detecting immunoglobulin G (IgG), the most important immunoglobulin. The immunosensor ability, however, significantly depends on the immobilization procedure. In this work, the effect of the nonspecific immobilization procedure on the sensor sensitivity is studied using a homebuilt electrodeless quartz-crystal microbalance (QCM) biosensor. The pure-shear vibration of a 9.7-mm-thick AT-cut quartz plate is excited and detected in liquids by the line antenna located outside the flow channel. SPA molecules are immobilized on the quartz surfaces, and human IgG is injected to monitor the binding reaction between SPA and IgG. This study reveals that a long (nearly 24 h) immersion procedure is required for immobilizing SPA to achieve the tight biding with the quartz surfaces.
DC.format	84165
citation_title	Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins
citation_author	Ogi, Hirotsugu
	Nagai, Hironao
	Fukunishi, Yuji
	Yanagida, Taiji
	Hirao, Masahiko
	Nishiyama, Masayoshi
citation_publisher	The Japan Society of Applied Physics
citation_language	英語
citation_date	2010-07-20
citation_journal_title	Japanese Journal of Applied Physics
citation_volume	49
citation_firstpage	07HD07
citation_doi	https://doi.org/10.1143/JJAP.49.07HD07
citation_public_url	https://hdl.handle.net/11094/84165

Development of 170MHz electrodeless quartz-crystal microbalance immunosensor with nonspecifically immobilized receptor proteins

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