Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor

Shagawa, Tomohiro; Torii, Hiroomi; Kato, Fumihito; Ogi, Hirotsugu; Hirao, Masahiko

doi:https://doi.org/10.7567/JJAP.54.068001

Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor

Shagawa, Tomohiro; Torii, Hiroomi; Kato, Fumihito et al.

Japanese Journal of Applied Physics, 2015, 54, 068001

アクセス数:360件（2025-08-23 15:25 集計）

固定URL: https://hdl.handle.net/11094/84170

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タイトル	Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor
著者	著者名 Shagawa, Tomohiro
	著者名 Torii, Hiroomi
	NRID 1000070780170 1000070780170 著者名 Kato, Fumihito
	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 著者名 Ogi, Hirotsugu
	NRID 1000080112027 1000080112027 著者名 Hirao, Masahiko
内容	Tomohiro Shagawa, Hiroomi Torii, Fumihito Kato, Hirotsugu Ogi and Masahiko Hirao. Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor. Japanese Journal of Applied Physics, 2015, 54(6), 068001. https://doi.org/10.7567/JJAP.54.068001.
抄録	This study proposes a methodology for evaluating specific binding behavior between proteins using a resonance acoustic microbalance with a naked-embedded quartz (RAMNE-Q) biosensor. We simultaneously measured the frequency responses of fundamental (58 MHz) and third-order (174 MHz) modes during multi step injections of proteins and deduced the thickness and viscosity evolutions of the protein layer. The viscosity increases with the progress of the binding reaction in nonspecific binding, but it markedly decreases in specific-binding cases. Thus, the high-frequency RAMNE-Q biosensor can be a powerful tool for evaluating specificity between proteins without measuring dissipation.
出版者	The Japan Society of Applied Physics
収録物名	Japanese Journal of Applied Physics
巻(号)	54
ページ	068001
刊行年月	2015-05-19
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84170
PISSN	00214922
eISSN	13474065
NCID	AA12295836
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.7567/JJAP.54.068001
アクセス権	open access
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Japanese Journal of Applied Physics. 2015, 54, p. 068001
DC.title	Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor
DC.creator	Shagawa, Tomohiro
	Torii, Hiroomi
	Kato, Fumihito
	Ogi, Hirotsugu
	Hirao, Masahiko
DC.publisher	The Japan Society of Applied Physics
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2015-05-19
DC.identifier	https://doi.org/10.7567/JJAP.54.068001
DC.description	Tomohiro Shagawa, Hiroomi Torii, Fumihito Kato, Hirotsugu Ogi and Masahiko Hirao. Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor. Japanese Journal of Applied Physics, 2015, 54(6), 068001. https://doi.org/10.7567/JJAP.54.068001.
DCTERMS.abstract	This study proposes a methodology for evaluating specific binding behavior between proteins using a resonance acoustic microbalance with a naked-embedded quartz (RAMNE-Q) biosensor. We simultaneously measured the frequency responses of fundamental (58 MHz) and third-order (174 MHz) modes during multi step injections of proteins and deduced the thickness and viscosity evolutions of the protein layer. The viscosity increases with the progress of the binding reaction in nonspecific binding, but it markedly decreases in specific-binding cases. Thus, the high-frequency RAMNE-Q biosensor can be a powerful tool for evaluating specificity between proteins without measuring dissipation.
DC.format	84170
citation_title	Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor
citation_author	Shagawa, Tomohiro
	Torii, Hiroomi
	Kato, Fumihito
	Ogi, Hirotsugu
	Hirao, Masahiko
citation_publisher	The Japan Society of Applied Physics
citation_language	英語
citation_date	2015-05-19
citation_journal_title	Japanese Journal of Applied Physics
citation_volume	54
citation_firstpage	068001
citation_doi	https://doi.org/10.7567/JJAP.54.068001
citation_public_url	https://hdl.handle.net/11094/84170

Relationship between viscosity change and specificity in protein binding reaction studied by high-frequency wireless and electrodeless MEMS biosensor

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