Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation

Shagawa, Tomohiro; Torii, Hiroomi; Kato, Fumihito; Ogi, Hirotsugu; Hirao, Masahiko

doi:https://doi.org/10.7567/JJAP.54.096601

Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation

Shagawa, Tomohiro; Torii, Hiroomi; Kato, Fumihito et al.

Japanese Journal of Applied Physics, 2015, 54(9), 096601

アクセス数:374件（2025-08-23 15:25 集計）

固定URL: https://hdl.handle.net/11094/84172

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タイトル	Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation
著者	著者名 Shagawa, Tomohiro
	著者名 Torii, Hiroomi
	NRID 1000070780170 1000070780170 著者名 Kato, Fumihito
	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 著者名 Ogi, Hirotsugu
	NRID 1000080112027 1000080112027 著者名 Hirao, Masahiko
内容	Tomohiro Shagawa, Hiroomi Torii, Fumihito Kato, Hirotsugu Ogi and Masahiko Hirao. Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation. Japanese Journal of Applied Physics, 2015, 54(9), 096601. https://doi.org/10.7567/JJAP.54.096601.
抄録	In this study, we demonstrate the effectiveness of a resonance acoustic microbalance with a naked embedded quartz (RAMNE-Q) biosensor for evaluating viscoelastic property changes in thin protein layers during protein deposition reactions without dissipation measurement. Quartz crystal microbalance (QCM) biosensors have conventionally been adopted for the viscoelasticity evaluation of adsorbed protein layers by measuring dissipation as well as resonance frequency. However, dissipation, or the vibrational energy loss, is easily affected by many factors and is rarely measured with sufficiently high accuracy. To evaluate viscoelasticity only from a reliable frequency response, one needs to perform an ultrahighfrequency measurement, which is here achieved using the RAMNE-Q biosensor. Simultaneous frequency measurement is performed for fundamental and overtone modes up to 406MHz of a 58MHz RAMNE-Q biosensor during various binding reactions, and evolutions of viscosity, shear modulus, and thickness of adsorbed protein layers are inversely evaluated. A marked difference is observed in the viscosity evolution between specific and nonspecific binding reactions. Furthermore, the reversed frequency response appears, which indicates the modification of the protein structure into a rigid structure.
出版者	The Japan Society of Applied Physics
収録物名	Japanese Journal of Applied Physics
巻(号)	54(9)
ページ	096601
刊行年月	2015-08-06
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84172
PISSN	00214922
eISSN	13474065
NCID	AA12295836
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.7567/JJAP.54.096601
アクセス権	open access
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Japanese Journal of Applied Physics. 2015, 54(9), p. 096601
DC.title	Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation
DC.creator	Shagawa, Tomohiro
	Torii, Hiroomi
	Kato, Fumihito
	Ogi, Hirotsugu
	Hirao, Masahiko
DC.publisher	The Japan Society of Applied Physics
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2015-08-06
DC.identifier	https://doi.org/10.7567/JJAP.54.096601
DC.description	Tomohiro Shagawa, Hiroomi Torii, Fumihito Kato, Hirotsugu Ogi and Masahiko Hirao. Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation. Japanese Journal of Applied Physics, 2015, 54(9), 096601. https://doi.org/10.7567/JJAP.54.096601.
DCTERMS.abstract	In this study, we demonstrate the effectiveness of a resonance acoustic microbalance with a naked embedded quartz (RAMNE-Q) biosensor for evaluating viscoelastic property changes in thin protein layers during protein deposition reactions without dissipation measurement. Quartz crystal microbalance (QCM) biosensors have conventionally been adopted for the viscoelasticity evaluation of adsorbed protein layers by measuring dissipation as well as resonance frequency. However, dissipation, or the vibrational energy loss, is easily affected by many factors and is rarely measured with sufficiently high accuracy. To evaluate viscoelasticity only from a reliable frequency response, one needs to perform an ultrahighfrequency measurement, which is here achieved using the RAMNE-Q biosensor. Simultaneous frequency measurement is performed for fundamental and overtone modes up to 406MHz of a 58MHz RAMNE-Q biosensor during various binding reactions, and evolutions of viscosity, shear modulus, and thickness of adsorbed protein layers are inversely evaluated. A marked difference is observed in the viscosity evolution between specific and nonspecific binding reactions. Furthermore, the reversed frequency response appears, which indicates the modification of the protein structure into a rigid structure.
DC.format	84172
citation_title	Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation
citation_author	Shagawa, Tomohiro
	Torii, Hiroomi
	Kato, Fumihito
	Ogi, Hirotsugu
	Hirao, Masahiko
citation_publisher	The Japan Society of Applied Physics
citation_language	英語
citation_date	2015-08-06
citation_journal_title	Japanese Journal of Applied Physics
citation_volume	54
citation_issue	9
citation_firstpage	096601
citation_doi	https://doi.org/10.7567/JJAP.54.096601
citation_public_url	https://hdl.handle.net/11094/84172

Viscoelasticity evolution in protein layers during binding reactions evaluated using high-frequency wireless and electrodeless quartz crystal microbalance biosensor without dissipation

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