Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice

Oka, Yuichiro; Doi, Miyuki; Taniguchi, Manabu; Tiong, Sheena Y X; Akiyama, Hisanori; Yamamoto, Takuto; Iguchi, Tokuichi; Sato, Makoto

doi:https://doi.org/10.1093/cercor/bhab153

Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice

Oka, Yuichiro; Doi, Miyuki; Taniguchi, Manabu et al.

Cerebral Cortex, 2021, 31(11), 5225-5238

アクセス数:905件（2025-08-14 20:04 集計）

固定URL: https://hdl.handle.net/11094/84187

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タイトル	Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice
著者	NRID 1000030614432 1000030614432 researchmap permalink okay okay Scopus Author ID 36958214100 36958214100 著者名 Oka, Yuichiro
	著者名 Doi, Miyuki
	NRID 1000030397707 1000030397707 著者名 Taniguchi, Manabu
	著者名 Tiong, Sheena Y X
	著者名 Akiyama, Hisanori
	著者名 Yamamoto, Takuto
	NRID 1000060509305 1000060509305 著者名 Iguchi, Tokuichi
	NRID 1000010222019 1000010222019 ORCID 0000-0002-6805-8351 0000-0002-6805-8351 researchmap permalink read0185568 read0185568 Scopus Author ID 55588061100 55588061100 著者名 Sato, Makoto
キーワード等	barrel cortex
	corticocortical projection
	inter-areal connection
	optical tissue clearing
	sensorimotor integration
内容	Yuichiro Oka, Miyuki Doi, Manabu Taniguchi, Sheena Y X Tiong, Hisanori Akiyama, Takuto Yamamoto, Tokuichi Iguchi, Makoto Sato, Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice, Cerebral Cortex, 2021;, bhab153, https://doi.org/10.1093/cercor/bhab153.
抄録	Association projections from cortical pyramidal neurons connect disparate intrahemispheric cortical areas, which are implicated in higher cortical functions. The underlying developmental processes of these association projections, especially the initial phase before reaching the target areas, remain unknown. To visualize developing axons of individual neurons with association projections in the mouse neocortex, we devised a sparse labeling method that combined in utero electroporation and confocal imaging of flattened and optically cleared cortices. Using the promoter of an established callosal neuron marker gene that was expressed in over 80% of L2/3 neurons in the primary somatosensory cortex (S1) that project to the primary motor cortex (M1), we found that an association projection of a single neuron was the longest among the interstitial collaterals that branched out in L5 from the earlier-extended callosal projection. Collaterals to M1 elongated primarily within the cortical gray matter with little branching before reaching the target. Our results suggest that dual-projection neurons in S1 make a significant fraction of the association projections to M1, supporting the directed guidance mechanism in long-range corticocortical circuit formation over random projections followed by specific pruning.
出版者	Oxford University Press
収録物名	Cerebral Cortex
巻(号)	31(11)
ページ	5225-5238
刊行年月	2021-07-06
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84187
PISSN	10473211
eISSN	14602199
NCID	AA10777916
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1093/cercor/bhab153
アクセス権	open access
権利情報	© 2021 The Author(s). Published by Oxford University Press. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Cerebral Cortex. 2021, 31(11), p. 5225-5238
DC.title	Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice
DC.creator	Oka, Yuichiro
	Doi, Miyuki
	Taniguchi, Manabu
	Tiong, Sheena Y X
	Akiyama, Hisanori
	Yamamoto, Takuto
	Iguchi, Tokuichi
	Sato, Makoto
DC.publisher	Oxford University Press
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-07-06
DC.identifier	https://doi.org/10.1093/cercor/bhab153
DC.subject	barrel cortex
	corticocortical projection
	inter-areal connection
	optical tissue clearing
	sensorimotor integration
DC.description	Yuichiro Oka, Miyuki Doi, Manabu Taniguchi, Sheena Y X Tiong, Hisanori Akiyama, Takuto Yamamoto, Tokuichi Iguchi, Makoto Sato, Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice, Cerebral Cortex, 2021;, bhab153, https://doi.org/10.1093/cercor/bhab153.
DCTERMS.abstract	Association projections from cortical pyramidal neurons connect disparate intrahemispheric cortical areas, which are implicated in higher cortical functions. The underlying developmental processes of these association projections, especially the initial phase before reaching the target areas, remain unknown. To visualize developing axons of individual neurons with association projections in the mouse neocortex, we devised a sparse labeling method that combined in utero electroporation and confocal imaging of flattened and optically cleared cortices. Using the promoter of an established callosal neuron marker gene that was expressed in over 80% of L2/3 neurons in the primary somatosensory cortex (S1) that project to the primary motor cortex (M1), we found that an association projection of a single neuron was the longest among the interstitial collaterals that branched out in L5 from the earlier-extended callosal projection. Collaterals to M1 elongated primarily within the cortical gray matter with little branching before reaching the target. Our results suggest that dual-projection neurons in S1 make a significant fraction of the association projections to M1, supporting the directed guidance mechanism in long-range corticocortical circuit formation over random projections followed by specific pruning.
DC.rights	© 2021 The Author(s). Published by Oxford University Press. This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	84187
citation_title	Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice
citation_author	Oka, Yuichiro
	Doi, Miyuki
	Taniguchi, Manabu
	Tiong, Sheena Y X
	Akiyama, Hisanori
	Yamamoto, Takuto
	Iguchi, Tokuichi
	Sato, Makoto
citation_publisher	Oxford University Press
citation_language	英語
citation_date	2021-07-06
citation_journal_title	Cerebral Cortex
citation_volume	31
citation_issue	11
citation_firstpage	5225
citation_lastpage	5238
citation_doi	https://doi.org/10.1093/cercor/bhab153
citation_public_url	https://hdl.handle.net/11094/84187

Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice

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