学術雑誌論文

学術雑誌論文

text

Biosensors and Bioelectronics. 2011, 26(12), p. 4819-4822

Replacement-free mass-amplified sandwich assay with 180-MHz electrodeless quartz-crystal microbalance biosensor

Elsevier B.V.

英語

text

2011-05-30

https://doi.org/10.1016/j.bios.2011.05.035

This study describes a sensitivity-amplified detection method in a replacement-free electrodeless quartz-crystal microbalance (QCM) biosensor. A sandwich assay is proposed for detecting C-reactive proteins (CRP), where the biotinated second anti-CRP antibody is weighted by streptavidin for sensitivity amplification. Because the first CRP antibody was immobilized nonspecifically on naked quartz surfaces, the sandwich assay was repeated using the same sensor chip, making possible the replacement-free assay. The mass-amplified sandwich assay detected a CRP solution of 0.1. ng/ml. A methodology for determining the molecular mass of the injected protein is also proposed. © 2011 Elsevier B.V.

© 2011 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

84194

Replacement-free mass-amplified sandwich assay with 180-MHz electrodeless quartz-crystal microbalance biosensor

Elsevier B.V.

英語

2011-05-30

Biosensors and Bioelectronics

26

12

4819

4822

https://doi.org/10.1016/j.bios.2011.05.035

21680171

https://hdl.handle.net/11094/84194