Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor

Ogi, Hirotsugu; Fukushima, Masahiko; Uesugi, Kentaro; Yagi, Hisashi; Goto, Yuji; Hirao, Masahiko

doi:https://doi.org/10.1016/j.bios.2012.07.019

Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor

Ogi, Hirotsugu; Fukushima, Masahiko; Uesugi, Kentaro et al.

Biosensors and Bioelectronics, 2012, 40(1), 200-205

アクセス数:371件（2025-08-23 15:58 集計）

固定URL: https://hdl.handle.net/11094/84196

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タイトル	Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor
著者	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 著者名 Ogi, Hirotsugu
	著者名 Fukushima, Masahiko
	著者名 Uesugi, Kentaro
	NRID 1000010432494 1000010432494 著者名 Yagi, Hisashi
	NRID 1000040153770 1000040153770 researchmap permalink read0014440 read0014440 著者名 Goto, Yuji
	NRID 1000080112027 1000080112027 著者名 Hirao, Masahiko
キーワード等	Aβ
	Deposition rate
	Oligomer
	QCM
	Ultrasonication
抄録	High-frequency (~ 55MHz) wireless quartz-crystal microbalance biosensor was used for studying heterogeneous deposition behavior of AΒ1-40 peptide on AΒ1-42 nuclei, which were grown under the stirring agitation and 200-kHz ultrasonication at pH 2.2, 4.6, and 7.4. The deposition reaction was monitored over 40h, and the deposition rate was deduced. Among the agitation nuclei, the maximum deposition rate was observed on the nucleus grown at pH 4.6. However, ultrasonication nucleus grown at pH 7.4 produced much larger deposition rate, despite the same Β-sheet concentration. This result indicates that local structural modulation is caused in the nucleus by ultrasonication, which adsorbs the AΒ peptide more actively than other nuclei. The resultant deposits clearly show oligomeric structure. © 2012 Elsevier B.V.
出版者	Elsevier B.V.
収録物名	Biosensors and Bioelectronics
巻(号)	40(1)
ページ	200-205
刊行年月	2012-07-21
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84196
PISSN	09565663
eISSN	18734235
NCID	AA10739666
NCID	AA1152282X
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1016/j.bios.2012.07.019
関連情報 (isIdenticalTo)	PMID 22857904
アクセス権	open access
権利情報	© 2012 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biosensors and Bioelectronics. 2012, 40(1), p. 200-205
DC.title	Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor
DC.creator	Ogi, Hirotsugu
	Fukushima, Masahiko
	Uesugi, Kentaro
	Yagi, Hisashi
	Goto, Yuji
	Hirao, Masahiko
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2012-07-21
DC.identifier	https://doi.org/10.1016/j.bios.2012.07.019
DC.subject	Aβ
	Deposition rate
	Oligomer
	QCM
	Ultrasonication
DCTERMS.abstract	High-frequency (~ 55MHz) wireless quartz-crystal microbalance biosensor was used for studying heterogeneous deposition behavior of AΒ1-40 peptide on AΒ1-42 nuclei, which were grown under the stirring agitation and 200-kHz ultrasonication at pH 2.2, 4.6, and 7.4. The deposition reaction was monitored over 40h, and the deposition rate was deduced. Among the agitation nuclei, the maximum deposition rate was observed on the nucleus grown at pH 4.6. However, ultrasonication nucleus grown at pH 7.4 produced much larger deposition rate, despite the same Β-sheet concentration. This result indicates that local structural modulation is caused in the nucleus by ultrasonication, which adsorbs the AΒ peptide more actively than other nuclei. The resultant deposits clearly show oligomeric structure. © 2012 Elsevier B.V.
DC.rights	© 2012 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	84196
citation_title	Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor
citation_author	Ogi, Hirotsugu
	Fukushima, Masahiko
	Uesugi, Kentaro
	Yagi, Hisashi
	Goto, Yuji
	Hirao, Masahiko
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2012-07-21
citation_journal_title	Biosensors and Bioelectronics
citation_volume	40
citation_issue	1
citation_firstpage	200
citation_lastpage	205
citation_doi	https://doi.org/10.1016/j.bios.2012.07.019
citation_pmid	22857904
citation_public_url	https://hdl.handle.net/11094/84196

Acceleration of deposition of AΒ1-40 peptide on ultrasonically formed AΒ1-42 nucleus studied by wireless quartz-crystal-microbalance biosensor

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