Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM

Ogi, Hirotsugu; Motohisa, Kazuma; Hatanaka, Kenichi; Ohmori, Toshinobu; Hirao, Masahiko; Nishiyama, Masayoshi

doi:https://doi.org/10.1016/j.bios.2007.03.003

Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM

Ogi, Hirotsugu; Motohisa, Kazuma; Hatanaka, Kenichi et al.

Biosensors and Bioelectronics, 2007, 22(12), 3238-3242

アクセス数:866件（2025-08-23 15:26 集計）

固定URL: https://hdl.handle.net/11094/84472

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タイトル	Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM
著者	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 著者名 Ogi, Hirotsugu
	著者名 Motohisa, Kazuma
	著者名 Hatanaka, Kenichi
	著者名 Ohmori, Toshinobu
	NRID 1000080112027 1000080112027 著者名 Hirao, Masahiko
	著者名 Nishiyama, Masayoshi
キーワード等	Affinity
	Electrodeless
	QCM
	IgG
	Protein A
	Wireless
抄録	The binding affinity between human immunoglobulin G (IgG) and protein A was studied by the homebuilt wireless-electrodeless quartz crystal microbalance (QCM). Protein A was immobilized on the electrodeless AT-cut quartz plate of 0.05 mm thick and its fundamental resonance frequency near 34 MHz was measured by a noncontacting manner using a line antenna. The vibrational analysis was performed to ensure higher sensitivity of the electrodeless QCM. A flow-cell system was fabricated to continuously measure the resonance frequency during the injection sequence of the IgG solutions with concentrations of 1-20,000 ng/mL. The exponential frequency changes were recorded to determine the affinity based on the Langmuir kinetics. The equilibrium constant KA significantly varied between 6×10⁶ and 6×10¹⁰ M-¹, depending on the IgG concentration, which is attributed to various formations of IgG-protein A complexes.
出版者	Elsevier B.V.
収録物名	Biosensors and Bioelectronics
巻(号)	22(12)
ページ	3238-3242
刊行年月	2007-06-15
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84472
PISSN	09565663
NCID	AA10739666
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1016/j.bios.2007.03.003
関連情報 (isIdenticalTo)	PMID 17420120
アクセス権	open access
権利情報	© 2007 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biosensors and Bioelectronics. 2007, 22(12), p. 3238-3242
DC.title	Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM
DC.creator	Ogi, Hirotsugu
	Motohisa, Kazuma
	Hatanaka, Kenichi
	Ohmori, Toshinobu
	Hirao, Masahiko
	Nishiyama, Masayoshi
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2007-06-15
DC.identifier	https://doi.org/10.1016/j.bios.2007.03.003
DC.subject	Affinity
	Electrodeless
	QCM
	IgG
	Protein A
	Wireless
DCTERMS.abstract	The binding affinity between human immunoglobulin G (IgG) and protein A was studied by the homebuilt wireless-electrodeless quartz crystal microbalance (QCM). Protein A was immobilized on the electrodeless AT-cut quartz plate of 0.05 mm thick and its fundamental resonance frequency near 34 MHz was measured by a noncontacting manner using a line antenna. The vibrational analysis was performed to ensure higher sensitivity of the electrodeless QCM. A flow-cell system was fabricated to continuously measure the resonance frequency during the injection sequence of the IgG solutions with concentrations of 1-20,000 ng/mL. The exponential frequency changes were recorded to determine the affinity based on the Langmuir kinetics. The equilibrium constant KA significantly varied between 6×10⁶ and 6×10¹⁰ M-¹, depending on the IgG concentration, which is attributed to various formations of IgG-protein A complexes.
DC.rights	© 2007 Elsevier B.V. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	84472
citation_title	Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM
citation_author	Ogi, Hirotsugu
	Motohisa, Kazuma
	Hatanaka, Kenichi
	Ohmori, Toshinobu
	Hirao, Masahiko
	Nishiyama, Masayoshi
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2007-06-15
citation_journal_title	Biosensors and Bioelectronics
citation_volume	22
citation_issue	12
citation_firstpage	3238
citation_lastpage	3242
citation_doi	https://doi.org/10.1016/j.bios.2007.03.003
citation_pmid	17420120
citation_public_url	https://hdl.handle.net/11094/84472

Concentration dependence of IgG-protein A affinity studied by wireless-electrodeless QCM

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