Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor

Noi, Kentaro; Iijima, Masumi; Kuroda, Shun-ichi; Kato, Fumihito; Ogi, Hirotsugu

doi:https://doi.org/10.35848/1347-4065/ab78e1

Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor

Noi, Kentaro; Iijima, Masumi; Kuroda, Shun-ichi et al.

Japanese Journal of Applied Physics, 2020, 59(SK), SKKB03-1-SKKB03-4

アクセス数:864件（2025-08-23 13:54 集計）

固定URL: https://hdl.handle.net/11094/84475

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タイトル	Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor
著者	NRID 1000030588405 1000030588405 著者名 Noi, Kentaro
	NRID 1000040390728 1000040390728 著者名 Iijima, Masumi
	NRID 1000060263406 1000060263406 researchmap permalink read0186039 read0186039 著者名 Kuroda, Shun-ichi
	NRID 1000070780170 1000070780170 著者名 Kato, Fumihito
	NRID 1000090252626 1000090252626 researchmap permalink read0042771 read0042771 Scopus Author ID 35563683500 35563683500 著者名 Ogi, Hirotsugu
内容	This is the Accepted Manuscript version of an article accepted for publication in Japanese Journal of Applied Physics. IOP Publishing Ltd are not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.35848/1347-4065/ab78e1.
抄録	Recent advances in functionalized bio-nanocapsules (BNCs) have allowed a significant sensitivity enhancement in label-free biosensors. It is suggested that sensitivity amplification is caused by the higher binding affinity of BNCs to target antibodies. We here study the high-affinity interaction mechanism between BNCs and antibodies with wireless high-frequency QCM biosensors. We first confirmed the higher affinity between BNCs and human immunoglobulin G. We then found that the number of binding sites for the target antibody significantly increases by immobilizing BNC molecules on the sensor surface. We finally studied changes in viscoelasticity near the sensor surface using a MEMS wireless QCM biosensor using up to the ninth overtone (522 MHz). The inversely determined effective shear modulus on BNCs was found to be significantly lower than that on a standard surface on which the receptor molecules were immobilized. We have thus clarified that this surface flexibility achieves high affinity with target antibodies.
出版者	Institute of Physics Publishing
収録物名	Japanese Journal of Applied Physics
巻(号)	59(SK)
ページ	SKKB03-1-SKKB03-4
刊行年月	2020-03-18
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84475
PISSN	00214922
eISSN	13474065
NCID	AA12295836
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.35848/1347-4065/ab78e1
アクセス権	open access
権利情報	© 2020 The Japan Society of Applied Physics. This Accepted Manuscript is available for reuse under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License after the 12 month embargo period provided that all the terms of the licence are adhered to.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Japanese Journal of Applied Physics. 2020, 59(SK), p. SKKB03-1-SKKB03-4
DC.title	Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor
DC.creator	Noi, Kentaro
	Iijima, Masumi
	Kuroda, Shun-ichi
	Kato, Fumihito
	Ogi, Hirotsugu
DC.publisher	Institute of Physics Publishing
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-03-18
DC.identifier	https://doi.org/10.35848/1347-4065/ab78e1
DC.description	This is the Accepted Manuscript version of an article accepted for publication in Japanese Journal of Applied Physics. IOP Publishing Ltd are not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.35848/1347-4065/ab78e1.
DCTERMS.abstract	Recent advances in functionalized bio-nanocapsules (BNCs) have allowed a significant sensitivity enhancement in label-free biosensors. It is suggested that sensitivity amplification is caused by the higher binding affinity of BNCs to target antibodies. We here study the high-affinity interaction mechanism between BNCs and antibodies with wireless high-frequency QCM biosensors. We first confirmed the higher affinity between BNCs and human immunoglobulin G. We then found that the number of binding sites for the target antibody significantly increases by immobilizing BNC molecules on the sensor surface. We finally studied changes in viscoelasticity near the sensor surface using a MEMS wireless QCM biosensor using up to the ninth overtone (522 MHz). The inversely determined effective shear modulus on BNCs was found to be significantly lower than that on a standard surface on which the receptor molecules were immobilized. We have thus clarified that this surface flexibility achieves high affinity with target antibodies.
DC.rights	© 2020 The Japan Society of Applied Physics. This Accepted Manuscript is available for reuse under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License after the 12 month embargo period provided that all the terms of the licence are adhered to.
DC.format	84475
citation_title	Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor
citation_author	Noi, Kentaro
	Iijima, Masumi
	Kuroda, Shun-ichi
	Kato, Fumihito
	Ogi, Hirotsugu
citation_publisher	Institute of Physics Publishing
citation_language	英語
citation_date	2020-03-18
citation_journal_title	Japanese Journal of Applied Physics
citation_volume	59
citation_issue	SK
citation_firstpage	SKKB03-1
citation_lastpage	SKKB03-4
citation_doi	https://doi.org/10.35848/1347-4065/ab78e1
citation_public_url	https://hdl.handle.net/11094/84475

Mechanism of affinity-enhanced protein adsorption on bio-nanocapsules studied by viscoelasticity measurement with wireless QCM biosensor

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