Kinetics of denaturation and renaturation processes of double-stranded helical polysaccharide, xanthan in aqueous sodium chloride

Tomofuji, Yu; Matsuo, Koichi; Terao, Ken

doi:https://doi.org/10.1016/j.carbpol.2021.118681

Kinetics of denaturation and renaturation processes of double-stranded helical polysaccharide, xanthan in aqueous sodium chloride

Tomofuji, Yu; Matsuo, Koichi; Terao, Ken

Carbohydrate Polymers, 2021, 275, 118681

アクセス数:553件（2026-02-16 11:42 集計）

固定URL: https://hdl.handle.net/11094/84559

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タイトル	Kinetics of denaturation and renaturation processes of double-stranded helical polysaccharide, xanthan in aqueous sodium chloride
著者	著者名 Tomofuji, Yu
	NRID 1000040403620 1000040403620 著者名 Matsuo, Koichi
	NRID 1000060334132 1000060334132 ORCID 0000-0001-7363-4491 0000-0001-7363-4491 researchmap permalink kenterao kenterao Scopus Author ID 7101653015 7101653015 著者名 Terao, Ken
キーワード等	double helix
	conformational change
	small-angle X-ray scattering
	circular dichroism
	intermediate state
内容	The definition of α in page 13 is incorrect. The right definition of α is the fraction of the double helix.
抄録	Circular dichroism (CD) and small-angle X-ray scattering (SAXS) measurements were made for three xanthan samples, a double helical polysaccharide, in 5 or 10 mM aqueous NaCl after rapid temperature change to investigate the kinetics of the conformational change between the ordered and disordered states. After the rapid heating, the CD signal mainly reflecting the carbonyl groups on the side chains quickly changed (<150 s) while the scattering intensity from SAXS around q (magnitude of the scattering vector) = 1 nm−1 changed more gradually, reflecting the main-chain conformation. The difference between CD and SAXS implies us the intermediate conformation which can be regarded as a loose double helix. The SAXS profile in the rapid cooling process showed that the loose double helical structure was constructed within 150 s, but the CD signal slowly changed with around 2 days to recover the native tight double helix.
出版者	Elsevier Ltd
収録物名	Carbohydrate Polymers
巻(号)	275
ページ	118681
刊行年月	2021-09-21
言語	英語
ハンドルURL	https://hdl.handle.net/11094/84559
PISSN	01448617
NCID	AA10626772
NCID	AA11522761
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1016/j.carbpol.2021.118681
アクセス権	open access
権利情報	© 2021 Elsevier Ltd. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Carbohydrate Polymers. 2021, 275, p. 118681
DC.title	Kinetics of denaturation and renaturation processes of double-stranded helical polysaccharide, xanthan in aqueous sodium chloride
DC.creator	Tomofuji, Yu
	Matsuo, Koichi
	Terao, Ken
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-09-21
DC.identifier	https://doi.org/10.1016/j.carbpol.2021.118681
DC.subject	double helix
	conformational change
	small-angle X-ray scattering
	circular dichroism
	intermediate state
DC.description	The definition of α in page 13 is incorrect. The right definition of α is the fraction of the double helix.
DCTERMS.abstract	Circular dichroism (CD) and small-angle X-ray scattering (SAXS) measurements were made for three xanthan samples, a double helical polysaccharide, in 5 or 10 mM aqueous NaCl after rapid temperature change to investigate the kinetics of the conformational change between the ordered and disordered states. After the rapid heating, the CD signal mainly reflecting the carbonyl groups on the side chains quickly changed (<150 s) while the scattering intensity from SAXS around q (magnitude of the scattering vector) = 1 nm−1 changed more gradually, reflecting the main-chain conformation. The difference between CD and SAXS implies us the intermediate conformation which can be regarded as a loose double helix. The SAXS profile in the rapid cooling process showed that the loose double helical structure was constructed within 150 s, but the CD signal slowly changed with around 2 days to recover the native tight double helix.
DC.rights	© 2021 Elsevier Ltd. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	84559
citation_title	Kinetics of denaturation and renaturation processes of double-stranded helical polysaccharide, xanthan in aqueous sodium chloride
citation_author	Tomofuji, Yu
	Matsuo, Koichi
	Terao, Ken
citation_publisher	Elsevier Ltd
citation_language	英語
citation_date	2021-09-21
citation_journal_title	Carbohydrate Polymers
citation_volume	275
citation_firstpage	118681
citation_doi	https://doi.org/10.1016/j.carbpol.2021.118681
citation_public_url	https://hdl.handle.net/11094/84559

Kinetics of denaturation and renaturation processes of double-stranded helical polysaccharide, xanthan in aqueous sodium chloride

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