Ultrasound inflammation imaging in rats with myocardial ischemia-reperfusion: Evaluation by non-specific targeted contrast microbubbles

Asanuma, Toshihiko; Mabuchi, Ryu; Masuda, Kasumi; Ueda, Hatsue; Nakatani, Satoshi

doi:https://doi.org/10.1007/s12574-010-0051-z

Ultrasound inflammation imaging in rats with myocardial ischemia-reperfusion: Evaluation by non-specific targeted contrast microbubbles

Asanuma, Toshihiko; Mabuchi, Ryu; Masuda, Kasumi et al.

Journal of Echocardiography, 2010, 8(4), 101-105

アクセス数:297件（2025-07-08 16:28 集計）

固定URL: https://hdl.handle.net/11094/86877

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タイトル	Ultrasound inflammation imaging in rats with myocardial ischemia-reperfusion: Evaluation by non-specific targeted contrast microbubbles
著者	NRID 1000080379271 1000080379271 著者名 Asanuma, Toshihiko
	著者名 Mabuchi, Ryu
	NRID 1000020533293 1000020533293 著者名 Masuda, Kasumi
	NRID 1000040522983 1000040522983 著者名 Ueda, Hatsue 著者の別表記 Ishibashi, Hatsue
	NRID 1000080393221 1000080393221 著者名 Nakatani, Satoshi
キーワード等	Contrast
	Echocardiography
	Inflammation
	Microbubble
内容	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s12574-010-0051-z.
抄録	Background: Reports on ultrasound inflammation imaging with non-specific targeted microbubbles in the heart have been scarce. We investigated whether inflammation induced by myocardial ischemia-reperfusion in rats could be evaluated by ultrasound inflammation imaging with non-specific targeted microbubbles. Methods: Six rats subjected to 30 min of occlusion of the left anterior descending artery (LAD) followed by 4 h of reperfusion (ischemia group) and 4 rats subjected to the sham operation (sham group) were used. Ultrasound inflammation imaging was performed 4 h after reperfusion, and non-circulating signal intensity (SI), which reflects the signal derived from microbubbles phagocytosed by neutrophils in inflamed tissue, was calculated by the SI difference between the initial and subsequent imaging both in the LAD and non-LAD areas. The accumulation of neutrophils was confirmed by myeloperoxidase (MPO) staining. Results: Non-circulating SI in the LAD area was significantly greater for the ischemia group than the sham group [5.19 ± 2.19 (ischemia) vs. 0.31 ± 0.13 (sham) dB, p < 0.01]. Non-circulating SI in the LAD area was significantly higher than that in the non-LAD area when compared in the same rat of the ischemia group [5.19 ± 2.19 (LAD) vs. 0.18 ± 0.64 (non-LAD) dB, p < 0.01]. MPO-positive cells were confirmed in the LAD area of the ischemia group. Conclusion: Inflammation induced by myocardial ischemia-reperfusion in rats could be quantitatively assessed by ultrasound inflammation imaging with non-specific targeted microbubbles.
出版者	Japanese Society of Echocardiography
収録物名	Journal of Echocardiography
巻(号)	8(4)
ページ	101-105
刊行年月	2010-12
言語	英語
ハンドルURL	https://hdl.handle.net/11094/86877
PISSN	13490222
eISSN	1880344X
NCID	AA12042477
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1007/s12574-010-0051-z
アクセス権	open access
権利情報	© Japanese Society of Echocardiography 2010
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Echocardiography. 2010, 8(4), p. 101-105
DC.title	Ultrasound inflammation imaging in rats with myocardial ischemia-reperfusion: Evaluation by non-specific targeted contrast microbubbles
DC.creator	Asanuma, Toshihiko
	Mabuchi, Ryu
	Masuda, Kasumi
	Ueda, Hatsue
	Nakatani, Satoshi
DC.creator	Ishibashi, Hatsue
DC.publisher	Japanese Society of Echocardiography
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2010-12
DC.identifier	https://doi.org/10.1007/s12574-010-0051-z
DC.subject	Contrast
	Echocardiography
	Inflammation
	Microbubble
DC.description	This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s12574-010-0051-z.
DCTERMS.abstract	Background: Reports on ultrasound inflammation imaging with non-specific targeted microbubbles in the heart have been scarce. We investigated whether inflammation induced by myocardial ischemia-reperfusion in rats could be evaluated by ultrasound inflammation imaging with non-specific targeted microbubbles. Methods: Six rats subjected to 30 min of occlusion of the left anterior descending artery (LAD) followed by 4 h of reperfusion (ischemia group) and 4 rats subjected to the sham operation (sham group) were used. Ultrasound inflammation imaging was performed 4 h after reperfusion, and non-circulating signal intensity (SI), which reflects the signal derived from microbubbles phagocytosed by neutrophils in inflamed tissue, was calculated by the SI difference between the initial and subsequent imaging both in the LAD and non-LAD areas. The accumulation of neutrophils was confirmed by myeloperoxidase (MPO) staining. Results: Non-circulating SI in the LAD area was significantly greater for the ischemia group than the sham group [5.19 ± 2.19 (ischemia) vs. 0.31 ± 0.13 (sham) dB, p < 0.01]. Non-circulating SI in the LAD area was significantly higher than that in the non-LAD area when compared in the same rat of the ischemia group [5.19 ± 2.19 (LAD) vs. 0.18 ± 0.64 (non-LAD) dB, p < 0.01]. MPO-positive cells were confirmed in the LAD area of the ischemia group. Conclusion: Inflammation induced by myocardial ischemia-reperfusion in rats could be quantitatively assessed by ultrasound inflammation imaging with non-specific targeted microbubbles.
DC.rights	© Japanese Society of Echocardiography 2010
DC.format	86877
citation_title	Ultrasound inflammation imaging in rats with myocardial ischemia-reperfusion: Evaluation by non-specific targeted contrast microbubbles
citation_author	Asanuma, Toshihiko
	Mabuchi, Ryu
	Masuda, Kasumi
	Ueda, Hatsue
	Nakatani, Satoshi
citation_publisher	Japanese Society of Echocardiography
citation_language	英語
citation_date	2010-12
citation_journal_title	Journal of Echocardiography
citation_volume	8
citation_issue	4
citation_firstpage	101
citation_lastpage	105
citation_doi	https://doi.org/10.1007/s12574-010-0051-z
citation_public_url	https://hdl.handle.net/11094/86877

Ultrasound inflammation imaging in rats with myocardial ischemia-reperfusion: Evaluation by non-specific targeted contrast microbubbles

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