Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio

Koike, Kota; Smith, Nicholas; Fujita, Katsumasa

doi:https://doi.org/10.1117/12.2609736

Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio

Koike, Kota; Smith, Nicholas; Fujita, Katsumasa

Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2022, 11973, 1197308

アクセス数:503件（2025-08-16 10:07 集計）

固定URL: https://hdl.handle.net/11094/88581

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タイトル	Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio
著者	著者名 Koike, Kota
	NRID 1000010362592 1000010362592 ORCID 0000-0002-7835-199X 0000-0002-7835-199X researchmap permalink read0089935 read0089935 Scopus Author ID 55262815400 55262815400 著者名 Smith, Nicholas
	NRID 1000080362664 1000080362664 ORCID 0000-0002-2284-375X 0000-0002-2284-375X researchmap permalink read0089911 read0089911 Scopus Author ID 7404058829 7404058829 著者名 Fujita, Katsumasa
キーワード等	label-free
	pulse shaping
	Raman scattering
	spectral focusing
	stimulated Raman scattering microscopy
内容	SPIE BIOS, 22 January - 28 February 2022, San Francisco, California, United States
抄録	We propose the use of 14 ps chirped laser pulses in stimulated Raman scattering (SRS) microscopy to improve the spectral resolution and signal-to-background ratio (SBR) in SRS imaging. We developed a single-grating-based pulse chirper and implemented it into an intensity-modulation SRS microscope to stretch the excitation pulse width from 2 to 14 ps. We confirmed that the 14 ps pulses provide a spectral resolution of 2 cm-1 by measuring the SRS spectra of diamond crystals. We found that the 14 ps pulses have smaller nonlinear background signals and improve SBR in SRS imaging of various samples due to the instantaneous narrow-band excitation and low peak power. Our technique can broaden the application of the 2 ps intensity modulation SRS microscopy by improving the spectral resolution and sensitivity.
出版者	SPIE
収録物名	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
巻(号)	11973
ページ	1197308
刊行年月	2022-03-02
言語	英語
ハンドルURL	https://hdl.handle.net/11094/88581
PISSN	16057422
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1117/12.2609736
関連情報 (isIdenticalTo)	NCID BA51116822
アクセス権	open access
出版タイプ	Version of Record
カテゴリ	会議発表論文 Conference Paper

資源タイプ	会議発表論文
ローカル資源タイプ	会議発表論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 2022, 11973, p. 1197308
DC.title	Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio
DC.creator	Koike, Kota
	Smith, Nicholas
	Fujita, Katsumasa
DC.publisher	SPIE
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-03-02
DC.identifier	https://doi.org/10.1117/12.2609736
DC.subject	label-free
	pulse shaping
	Raman scattering
	spectral focusing
	stimulated Raman scattering microscopy
DC.description	SPIE BIOS, 22 January - 28 February 2022, San Francisco, California, United States
DCTERMS.abstract	We propose the use of 14 ps chirped laser pulses in stimulated Raman scattering (SRS) microscopy to improve the spectral resolution and signal-to-background ratio (SBR) in SRS imaging. We developed a single-grating-based pulse chirper and implemented it into an intensity-modulation SRS microscope to stretch the excitation pulse width from 2 to 14 ps. We confirmed that the 14 ps pulses provide a spectral resolution of 2 cm-1 by measuring the SRS spectra of diamond crystals. We found that the 14 ps pulses have smaller nonlinear background signals and improve SBR in SRS imaging of various samples due to the instantaneous narrow-band excitation and low peak power. Our technique can broaden the application of the 2 ps intensity modulation SRS microscopy by improving the spectral resolution and sensitivity.
DC.format	88581
citation_title	Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio
citation_author	Koike, Kota
	Smith, Nicholas
	Fujita, Katsumasa
citation_publisher	SPIE
citation_language	英語
citation_date	2022-03-02
citation_journal_title	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
citation_volume	11973
citation_firstpage	1197308
citation_doi	https://doi.org/10.1117/12.2609736
citation_public_url	https://hdl.handle.net/11094/88581

Stimulated Raman scattering microscopy with spectral focusing of 2-ps laser pulses for higher spectral resolution and signal-to-background ratio

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