学術雑誌論文

学術雑誌論文

text

PLoS ONE. 2014, 9(4), p. e94365

Evaluation of inferior alveolar nerve regeneration by bifocal distraction osteogenesis with retrograde transportation of horseradish peroxidase in dogs

Tanaka, Emiko

Public Library of Science

英語

text

2014-04-14

https://doi.org/10.1371/journal.pone.0094365

Evaluation of Inferior Alveolar Nerve Regeneration by Bifocal Distraction Osteogenesis with Retrograde Transportation of Horseradish Peroxidase in Dogs. Shogen Y, Isomura ET, Kogo M. PLOS ONE 2014. 9(4) e94365. doi:10.1371/journal.pone.0094365

Background: Bifocal distraction osteogenesis has been shown to be a reliable method for reconstructing segmental mandibular defects. However, there are few reports regarding the occurrence of inferior alveolar nerve regeneration during the process of distraction. Previously, we reported inferior alveolar nerve regeneration after distraction, and evaluated the regenerated nerve using histological and electrophysiological methods. In the present study, we investigated axons regenerated by bifocal distraction osteogenesis using retrograde transportation of horseradish peroxidase in the mandibles of dogs to determine their type and function. Methods and Findings: Using a bifocal distraction osteogenesis method, we produced a 10-mm mandibular defect, including a nerve defect, in 11 dogs and distracted using a transport disk at a rate of 1 mm/day. The regenerated inferior alveolar nerve was evaluated by retrograde transportation of HRP in all dogs at 3 and 6 months after the first operation. At 3 and 6 months, HRP-labeled neurons were observed in the trigeminal ganglion. The number of HRP-labeled neurons in each section increased, while the cell body diameter of HRP-labeled neurons was reduced over time. Conclusions: We found that the inferior alveolar nerve after bifocal distraction osteogenesis successfully recovered until peripheral tissue began to function. Although our research is still at the stage of animal experiments, it is considered that it will be possible to apply this method in the future to humans who have the mandibular defects. © 2014 Shogen et al.

This article is licensed under a Creative Commons Attribution 4.0 International License.

89688

Evaluation of inferior alveolar nerve regeneration by bifocal distraction osteogenesis with retrograde transportation of horseradish peroxidase in dogs

Public Library of Science

英語

2014-04-14

PLoS ONE

9

4

e94365

https://doi.org/10.1371/journal.pone.0094365

https://hdl.handle.net/11094/89688