Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration

Okamura, Gensuke; Ebina, Kosuke; Hirao, Makoto; Chijimatsu, Ryota; Yonetani, Yasukazu; Etani, Yuki; Miyama, Akira; Takami, Kenji; Goshima, Atsushi; Yoshikawa, Hideki; Ishimoto, Takuya; Nakano, Takayoshi; Hamada, Masayuki; Kanamoto, Takashi; Nakata, Ken

doi:https://doi.org/10.3390/ijms22010300

Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration

Okamura, Gensuke; Ebina, Kosuke; Hirao, Makoto et al.

International Journal of Molecular Sciences, 2020, 22(1)

アクセス数:260件（2025-04-03 22:14 集計）

固定URL: https://hdl.handle.net/11094/89793

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タイトル	Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration
著者	著者名 Okamura, Gensuke
	著者名 Ebina, Kosuke
	NRID 1000010527708 1000010527708 著者名 Hirao, Makoto
	著者名 Chijimatsu, Ryota
	NRID 1000080642090 1000080642090 著者名 Yonetani, Yasukazu
	NRID 1000060898930 1000060898930 著者名 Etani, Yuki
	著者名 Miyama, Akira
	著者名 Takami, Kenji
	著者名 Goshima, Atsushi
	NRID 1000060191558 1000060191558 著者名 Yoshikawa, Hideki
	NRID 1000050508835 1000050508835 ORCID 0000-0003-0081-0591 0000-0003-0081-0591 researchmap permalink i_taku i_taku Scopus Author ID 35310484700 35310484700 著者名 Ishimoto, Takuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 著者名 Nakano, Takayoshi
	著者名 Hamada, Masayuki
	NRID 1000020512049 1000020512049 researchmap permalink kanamoto kanamoto Scopus Author ID 7005012135 7005012135 著者名 Kanamoto, Takashi
	NRID 1000000283747 1000000283747 researchmap permalink ken-nakata ken-nakata Scopus Author ID 7402151308 7402151308 著者名 Nakata, Ken
キーワード等	Basic fibroblast growth factor
	Cartilage regenera-tion
	Synovial mesenchymal stem cells
	Xenograft
内容	Okamura G., Ebina K., Hirao M., et al. Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration. International Journal of Molecular Sciences, 22, 1, 1. https://doi.org/10.3390/ijms22010300.
抄録	Synovial mesenchymal stem cell (SMSC) is the promising cell source of cartilage regeneration but has several issues to overcome such as limited cell proliferation and heterogeneity of cartilage regeneration ability. Previous reports demonstrated that basic fibroblast growth factor (bFGF) can promote proliferation and cartilage differentiation potential of MSCs in vitro, although no reports show its beneficial effect in vivo. The purpose of this study is to investigate the promoting effect of bFGF on cartilage regeneration using human SMSC in vivo. SMSCs were cultured with or without bFGF in a growth medium, and 2 × 105 cells were aggregated to form a synovial pellet. Synovial pellets were implanted into osteochondral defects induced in the femoral trochlea of severe combined immunodeficient mice, and histological evaluation was performed after eight weeks. The presence of implanted SMSCs was confirmed by the observation of human vimentin immunostaining-positive cells. Interestingly, broad lacunae structures and cartilage substrate stained by Safranin-O were observed only in the bFGF (+) group. The bFGF (+) group had significantly higher O’Driscoll scores in the cartilage repair than the bFGF (−) group. The addition of bFGF to SMSC growth culture may be a useful treatment option to promote cartilage regeneration in vivo.
出版者	MDPI
収録物名	International Journal of Molecular Sciences
巻(号)	22(1)
刊行年月	2020-12-30
言語	英語
ハンドルURL	https://hdl.handle.net/11094/89793
PISSN	16616596
eISSN	14220067
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.3390/ijms22010300
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	International Journal of Molecular Sciences. 2020, 22(1)
DC.title	Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration
DC.creator	Okamura, Gensuke
	Ebina, Kosuke
	Hirao, Makoto
	Chijimatsu, Ryota
	Yonetani, Yasukazu
	Etani, Yuki
	Miyama, Akira
	Takami, Kenji
	Goshima, Atsushi
	Yoshikawa, Hideki
	Ishimoto, Takuya
	Nakano, Takayoshi
	Hamada, Masayuki
	Kanamoto, Takashi
	Nakata, Ken
DC.publisher	MDPI
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-12-30
DC.identifier	https://doi.org/10.3390/ijms22010300
DC.subject	Basic fibroblast growth factor
	Cartilage regenera-tion
	Synovial mesenchymal stem cells
	Xenograft
DC.description	Okamura G., Ebina K., Hirao M., et al. Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration. International Journal of Molecular Sciences, 22, 1, 1. https://doi.org/10.3390/ijms22010300.
DCTERMS.abstract	Synovial mesenchymal stem cell (SMSC) is the promising cell source of cartilage regeneration but has several issues to overcome such as limited cell proliferation and heterogeneity of cartilage regeneration ability. Previous reports demonstrated that basic fibroblast growth factor (bFGF) can promote proliferation and cartilage differentiation potential of MSCs in vitro, although no reports show its beneficial effect in vivo. The purpose of this study is to investigate the promoting effect of bFGF on cartilage regeneration using human SMSC in vivo. SMSCs were cultured with or without bFGF in a growth medium, and 2 × 105 cells were aggregated to form a synovial pellet. Synovial pellets were implanted into osteochondral defects induced in the femoral trochlea of severe combined immunodeficient mice, and histological evaluation was performed after eight weeks. The presence of implanted SMSCs was confirmed by the observation of human vimentin immunostaining-positive cells. Interestingly, broad lacunae structures and cartilage substrate stained by Safranin-O were observed only in the bFGF (+) group. The bFGF (+) group had significantly higher O’Driscoll scores in the cartilage repair than the bFGF (−) group. The addition of bFGF to SMSC growth culture may be a useful treatment option to promote cartilage regeneration in vivo.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	89793
citation_title	Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration
citation_author	Okamura, Gensuke
	Ebina, Kosuke
	Hirao, Makoto
	Chijimatsu, Ryota
	Yonetani, Yasukazu
	Etani, Yuki
	Miyama, Akira
	Takami, Kenji
	Goshima, Atsushi
	Yoshikawa, Hideki
	Ishimoto, Takuya
	Nakano, Takayoshi
	Hamada, Masayuki
	Kanamoto, Takashi
	Nakata, Ken
citation_publisher	MDPI
citation_language	英語
citation_date	2020-12-30
citation_journal_title	International Journal of Molecular Sciences
citation_volume	22
citation_issue	1
citation_doi	https://doi.org/10.3390/ijms22010300
citation_public_url	https://hdl.handle.net/11094/89793

Promoting effect of basic fibroblast growth factor in synovial mesenchymal stem cell-based cartilage regeneration

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