Bone apatite anisotropic structure control: Via designing fibrous scaffolds

Lee, Sungho; Nagata, Fukue; Kato, Katsuya; Nakano, Takayoshi

doi:https://doi.org/10.1039/d0ra01295e

Bone apatite anisotropic structure control: Via designing fibrous scaffolds

Lee, Sungho; Nagata, Fukue; Kato, Katsuya et al.

RSC Advances, 2020, 10(23), 13500-13506

アクセス数:107件（2025-04-07 01:34 集計）

固定URL: https://hdl.handle.net/11094/89807

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タイトル	Bone apatite anisotropic structure control: Via designing fibrous scaffolds
著者	NRID 1000020850001 1000020850001 著者名 Lee, Sungho
	NRID 1000000357633 1000000357633 著者名 Nagata, Fukue
	NRID 1000070356781 1000070356781 著者名 Kato, Katsuya
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 著者名 Nakano, Takayoshi
内容	Lee S., Nagata F., Kato K., et al. Bone apatite anisotropic structure control: Via designing fibrous scaffolds. RSC Advances, 10, 23, 13500. https://doi.org/10.1039/d0ra01295e.
抄録	Bone tissue has an anisotropic structure, associated with the collagen fibrils' orientation and the c-axis direction of the bone apatite crystal. The bone regeneration process comprises two main phases: bone mineral density restoration (bone quantity), and subsequent recovery of bone apatite c-axis orientation (bone quality). Bone quality is the determinant factor for mechanical properties of bone. Control of osteoblast alignment is one of the strategies for reconstructing bone quality since the collagen/apatite matrix orientation in calcified tissues is dependent on the osteoblast orientation. In this work, fibrous scaffolds designed for reconstruction of bone quality via cell alignment control was investigated. The fibrous scaffolds were fabricated using the electrospinning method with poly(lactic acid) at various fiber collecting speeds. The degree of fiber alignment in the prepared fibrous scaffolds increased with increasing fiber collecting speed, indicating that the fibers were oriented in a single direction. The alignment of osteoblasts on the fibrous scaffolds as well as the subsequent apatite c-axis orientation increased with increasing fiber collecting speed. We successfully controlled cell alignment and apatite c-axis orientation using the designed morphology of fibrous scaffolds. To the best of our knowledge, this is the first report demonstrating that adjusting the degree of fiber orientation for fibrous scaffolds can manipulate the regeneration of bone quality.
出版者	Royal Society of Chemistry
収録物名	RSC Advances
巻(号)	10(23)
ページ	13500-13506
刊行年月	2020-04-02
言語	英語
ハンドルURL	https://hdl.handle.net/11094/89807
eISSN	20462069
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1039/d0ra01295e
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 3.0 Unported License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

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資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	RSC Advances. 2020, 10(23), p. 13500-13506
DC.title	Bone apatite anisotropic structure control: Via designing fibrous scaffolds
DC.creator	Lee, Sungho
	Nagata, Fukue
	Kato, Katsuya
	Nakano, Takayoshi
DC.publisher	Royal Society of Chemistry
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-04-02
DC.identifier	https://doi.org/10.1039/d0ra01295e
DC.description	Lee S., Nagata F., Kato K., et al. Bone apatite anisotropic structure control: Via designing fibrous scaffolds. RSC Advances, 10, 23, 13500. https://doi.org/10.1039/d0ra01295e.
DCTERMS.abstract	Bone tissue has an anisotropic structure, associated with the collagen fibrils' orientation and the c-axis direction of the bone apatite crystal. The bone regeneration process comprises two main phases: bone mineral density restoration (bone quantity), and subsequent recovery of bone apatite c-axis orientation (bone quality). Bone quality is the determinant factor for mechanical properties of bone. Control of osteoblast alignment is one of the strategies for reconstructing bone quality since the collagen/apatite matrix orientation in calcified tissues is dependent on the osteoblast orientation. In this work, fibrous scaffolds designed for reconstruction of bone quality via cell alignment control was investigated. The fibrous scaffolds were fabricated using the electrospinning method with poly(lactic acid) at various fiber collecting speeds. The degree of fiber alignment in the prepared fibrous scaffolds increased with increasing fiber collecting speed, indicating that the fibers were oriented in a single direction. The alignment of osteoblasts on the fibrous scaffolds as well as the subsequent apatite c-axis orientation increased with increasing fiber collecting speed. We successfully controlled cell alignment and apatite c-axis orientation using the designed morphology of fibrous scaffolds. To the best of our knowledge, this is the first report demonstrating that adjusting the degree of fiber orientation for fibrous scaffolds can manipulate the regeneration of bone quality.
DC.rights	This article is licensed under a Creative Commons Attribution 3.0 Unported License.
DC.format	89807
citation_title	Bone apatite anisotropic structure control: Via designing fibrous scaffolds
citation_author	Lee, Sungho
	Nagata, Fukue
	Kato, Katsuya
	Nakano, Takayoshi
citation_publisher	Royal Society of Chemistry
citation_language	英語
citation_date	2020-04-02
citation_journal_title	RSC Advances
citation_volume	10
citation_issue	23
citation_firstpage	13500
citation_lastpage	13506
citation_doi	https://doi.org/10.1039/d0ra01295e
citation_public_url	https://hdl.handle.net/11094/89807