3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites

Matsugaki, Aira; Matsuzaka, Tadaaki; Murakami, Ami; Wang, Pan; Nakano, Takayoshi

3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites

Matsugaki, Aira; Matsuzaka, Tadaaki; Murakami, Ami et al.

International Journal of Bioprinting, 2020, 6(4), 293

アクセス数:474件（2025-10-24 13:15 集計）

固定URL: https://hdl.handle.net/11094/89810

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タイトル	3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites
著者	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 著者名 Matsugaki, Aira
	著者名 Matsuzaka, Tadaaki
	著者名 Murakami, Ami
	著者名 Wang, Pan
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 著者名 Nakano, Takayoshi
キーワード等	Bioprinting
	Collagen substrate
	Mineralization
	Osteocyte, 3D arrangement of bone matrix
	3D arrangement of bone matrix
内容	Matsugaki A., Matsuzaka T., Murakami A., et al. 3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites. International Journal of Bioprinting, 6, 4, 293. https://doi.org/10.18063/IJB.V6I4.293.
抄録	Although three-dimensional (3D) bioprinting techniques enable the construction of various living tissues and organs, the generation of bone-like oriented microstructures with anisotropic texture remains a challenge. Inside the mineralized bone matrix, osteocytes play mechanosensing roles in an ordered manner with a well-developed lacunar-canaliculi system. Therefore, control of cellular arrangement and dendritic processes is indispensable for construction of artificially controlled 3D bone-mimetic architecture. Herein, we propose an innovative methodology to induce controlled arrangement of osteocyte dendritic processes using the laminated layer method of oriented collagen sheets, combined with a custom-made fluid flow stimuli system. Osteocyte dendritic processes showed elongation depending on the competitive directional relationship between flow and substrate. To the best of our knowledge, this study is the first to report the successful construction of the anisotropic bone-mimetic microstructure and further demonstrate that the dendritic process formation in osteocytes can be controlled with selective fluid flow stimuli, specifically by regulating focal adhesion. Our results demonstrate how osteocytes adapt to mechanical stimuli by optimizing the anisotropic maturation of dendritic cell processes.
出版者	Whioce Publishing Pte.
収録物名	International Journal of Bioprinting
巻(号)	6(4)
ページ	293
刊行年月	2020-06-27
言語	英語
ハンドルURL	https://hdl.handle.net/11094/89810
eISSN	24248002
関連情報 (isIdenticalTo)	URI https://ijb.sg/index.php/int-j-bioprinting/article/view/293
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	International Journal of Bioprinting. 2020, 6(4), p. 293
DC.title	3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites
DC.creator	Matsugaki, Aira
	Matsuzaka, Tadaaki
	Murakami, Ami
	Wang, Pan
	Nakano, Takayoshi
DC.publisher	Whioce Publishing Pte.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2020-06-27
DC.relation' scheme='DCTERMS.URI	https://ijb.sg/index.php/int-j-bioprinting/article/view/293
DC.subject	Bioprinting
	Collagen substrate
	Mineralization
	Osteocyte, 3D arrangement of bone matrix
	3D arrangement of bone matrix
DC.description	Matsugaki A., Matsuzaka T., Murakami A., et al. 3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites. International Journal of Bioprinting, 6, 4, 293. https://doi.org/10.18063/IJB.V6I4.293.
DCTERMS.abstract	Although three-dimensional (3D) bioprinting techniques enable the construction of various living tissues and organs, the generation of bone-like oriented microstructures with anisotropic texture remains a challenge. Inside the mineralized bone matrix, osteocytes play mechanosensing roles in an ordered manner with a well-developed lacunar-canaliculi system. Therefore, control of cellular arrangement and dendritic processes is indispensable for construction of artificially controlled 3D bone-mimetic architecture. Herein, we propose an innovative methodology to induce controlled arrangement of osteocyte dendritic processes using the laminated layer method of oriented collagen sheets, combined with a custom-made fluid flow stimuli system. Osteocyte dendritic processes showed elongation depending on the competitive directional relationship between flow and substrate. To the best of our knowledge, this study is the first to report the successful construction of the anisotropic bone-mimetic microstructure and further demonstrate that the dendritic process formation in osteocytes can be controlled with selective fluid flow stimuli, specifically by regulating focal adhesion. Our results demonstrate how osteocytes adapt to mechanical stimuli by optimizing the anisotropic maturation of dendritic cell processes.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	89810
citation_title	3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites
citation_author	Matsugaki, Aira
	Matsuzaka, Tadaaki
	Murakami, Ami
	Wang, Pan
	Nakano, Takayoshi
citation_publisher	Whioce Publishing Pte.
citation_language	英語
citation_date	2020-06-27
citation_journal_title	International Journal of Bioprinting
citation_volume	6
citation_issue	4
citation_firstpage	293
citation_public_url	https://hdl.handle.net/11094/89810
citation_abstract_html_url	https://ijb.sg/index.php/int-j-bioprinting/article/view/293

3D printing of anisotropic bone-mimetic structure with controlled fluid flow stimuli for osteocytes: Flow orientation determines the elongation of dendrites

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