Biomimetic mineralization using matrix vesicle nanofragments

Kunitomi, Yosuke; Hara, Emilio Satoshi; Okada, Masahiro; Nagaoka, Noriyuki; Kuboki, Takuo; Nakano, Takayoshi; Kamioka, Hiroshi; Matsumoto, Takuya

doi:https://doi.org/10.1002/jbm.a.36618

Biomimetic mineralization using matrix vesicle nanofragments

Kunitomi, Yosuke; Hara, Emilio Satoshi; Okada, Masahiro et al.

Journal of Biomedical Materials Research - Part A, 2019, 107(5), 1021-1030

アクセス数:194件（2025-04-03 21:56 集計）

固定URL: https://hdl.handle.net/11094/89820

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タイトル	Biomimetic mineralization using matrix vesicle nanofragments
著者	著者名 Kunitomi, Yosuke
	NRID 1000040779443 1000040779443 著者名 Hara, Emilio Satoshi
	NRID 1000070416220 1000070416220 著者名 Okada, Masahiro
	NRID 1000070304326 1000070304326 著者名 Nagaoka, Noriyuki
	NRID 1000000225195 1000000225195 著者名 Kuboki, Takuo
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 著者名 Nakano, Takayoshi
	NRID 1000080253219 1000080253219 著者名 Kamioka, Hiroshi
	NRID 1000040324793 1000040324793 著者名 Matsumoto, Takuya
内容	Kunitomi Y., Hara E.S., Okada M., et al. Biomimetic mineralization using matrix vesicle nanofragments. Journal of Biomedical Materials Research - Part A, 107, 5, 1021. https://doi.org/10.1002/jbm.a.36618.
抄録	In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021–1030, 2019.
出版者	John Wiley and Sons
収録物名	Journal of Biomedical Materials Research - Part A
巻(号)	107(5)
ページ	1021-1030
刊行年月	2019-01-24
言語	英語
ハンドルURL	https://hdl.handle.net/11094/89820
PISSN	15493296
eISSN	15524965
NCID	AA11881516
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1002/jbm.a.36618
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Biomedical Materials Research - Part A. 2019, 107(5), p. 1021-1030
DC.title	Biomimetic mineralization using matrix vesicle nanofragments
DC.creator	Kunitomi, Yosuke
	Hara, Emilio Satoshi
	Okada, Masahiro
	Nagaoka, Noriyuki
	Kuboki, Takuo
	Nakano, Takayoshi
	Kamioka, Hiroshi
	Matsumoto, Takuya
DC.publisher	John Wiley and Sons
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2019-01-24
DC.identifier	https://doi.org/10.1002/jbm.a.36618
DC.description	Kunitomi Y., Hara E.S., Okada M., et al. Biomimetic mineralization using matrix vesicle nanofragments. Journal of Biomedical Materials Research - Part A, 107, 5, 1021. https://doi.org/10.1002/jbm.a.36618.
DCTERMS.abstract	In vitro synthesis of bone tissue has been paid attention in recent years; however, current methods to fabricate bone tissue are still ineffective due to some remaining gaps in the understanding of real in vivo bone formation process, and application of the knowledge in bone synthesis. Therefore, the objectives of this study were first, to perform a systematic and ultrastructural investigation of the initial mineral formation during intramembranous ossification of mouse calvaria from a material scientists' viewpoint, and to develop novel mineralization methods based on the in vivo findings. First, the very initial mineral deposition was found to occur at embryonic day E14.0 in mouse calvaria. Analysis of the initial bone formation process showed that it involved the following distinct steps: collagen secretion, matrix vesicle (MV) release, MV mineralization, MV rupture, and collagen fiber mineralization. Next, we performed in vitro mineralization experiments using MVs and hydrogel scaffolds. Intact MVs embedded in collagen gel did not mineralize, whereas, interestingly, MV nanofragments obtained by ultrasonication could promote rapid mineralization. These results indicate that mechanically ruptured MV membrane can be a promising material for in vitro bone tissue synthesis. © 2019 The Authors. journal Of Biomedical Materials Research Part A Published By Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1021–1030, 2019.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	89820
citation_title	Biomimetic mineralization using matrix vesicle nanofragments
citation_author	Kunitomi, Yosuke
	Hara, Emilio Satoshi
	Okada, Masahiro
	Nagaoka, Noriyuki
	Kuboki, Takuo
	Nakano, Takayoshi
	Kamioka, Hiroshi
	Matsumoto, Takuya
citation_publisher	John Wiley and Sons
citation_language	英語
citation_date	2019-01-24
citation_journal_title	Journal of Biomedical Materials Research - Part A
citation_volume	107
citation_issue	5
citation_firstpage	1021
citation_lastpage	1030
citation_doi	https://doi.org/10.1002/jbm.a.36618
citation_public_url	https://hdl.handle.net/11094/89820

Biomimetic mineralization using matrix vesicle nanofragments

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