Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model

Ozasa, Ryosuke; Matsugaki, Aira; Isobe, Yoshihiro; Saku, Taro; Yun, Hui Suk; Nakano, Takayoshi

doi:https://doi.org/10.1002/jbm.a.36238

Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model

Ozasa, Ryosuke; Matsugaki, Aira; Isobe, Yoshihiro et al.

Journal of Biomedical Materials Research - Part A, 2017, 106(2), 360-369

アクセス数:133件（2025-04-03 21:56 集計）

固定URL: https://hdl.handle.net/11094/89833

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タイトル	Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model
著者	NRID 1000080845347 1000080845347 ORCID 0000-0002-2773-072X 0000-0002-2773-072X researchmap permalink ozasa_r ozasa_r Scopus Author ID 56202812400 56202812400 著者名 Ozasa, Ryosuke
	NRID 1000010592529 1000010592529 ORCID 0000-0002-8427-7124 0000-0002-8427-7124 researchmap permalink aira aira Scopus Author ID 24172720900 24172720900 著者名 Matsugaki, Aira
	著者名 Isobe, Yoshihiro
	著者名 Saku, Taro
	著者名 Yun, Hui Suk
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 著者名 Nakano, Takayoshi
内容	Ozasa R., Matsugaki A., Isobe Y., et al. Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model. Journal of Biomedical Materials Research - Part A, 106, 2, 360. https://doi.org/10.1002/jbm.a.36238.
抄録	Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold. The oriented collagen scaffold allowed hiPSCs-derived osteoblast alignment and further construction of anisotropic bone matrix which mimics the bone tissue microstructure. To the best of our knowledge, this is the first report showing the construction of bone mimetic anisotropic bone matrix microstructure from hiPSCs. Moreover, we demonstrated for the first time that the hiPSCs-derived osteoblasts possess a high level of intact functionality to regulate cell alignment. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 360–369, 2018.
出版者	John Wiley and Sons
収録物名	Journal of Biomedical Materials Research - Part A
巻(号)	106(2)
ページ	360-369
刊行年月	2017-09-16
言語	英語
ハンドルURL	https://hdl.handle.net/11094/89833
PISSN	15493296
eISSN	15524965
NCID	AA11881516
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1002/jbm.a.36238
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Biomedical Materials Research - Part A. 2017, 106(2), p. 360-369
DC.title	Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model
DC.creator	Ozasa, Ryosuke
	Matsugaki, Aira
	Isobe, Yoshihiro
	Saku, Taro
	Yun, Hui Suk
	Nakano, Takayoshi
DC.publisher	John Wiley and Sons
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2017-09-16
DC.identifier	https://doi.org/10.1002/jbm.a.36238
DC.description	Ozasa R., Matsugaki A., Isobe Y., et al. Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model. Journal of Biomedical Materials Research - Part A, 106, 2, 360. https://doi.org/10.1002/jbm.a.36238.
DCTERMS.abstract	Bone tissue has anisotropic microstructure based on collagen/biological apatite orientation, which plays essential roles in the mechanical and biological functions of bone. However, obtaining an appropriate anisotropic microstructure during the bone regeneration process remains a great challenging. A powerful strategy for the control of both differentiation and structural development of newly-formed bone is required in bone tissue engineering, in order to realize functional bone tissue regeneration. In this study, we developed a novel anisotropic culture model by combining human induced pluripotent stem cells (hiPSCs) and artificially-controlled oriented collagen scaffold. The oriented collagen scaffold allowed hiPSCs-derived osteoblast alignment and further construction of anisotropic bone matrix which mimics the bone tissue microstructure. To the best of our knowledge, this is the first report showing the construction of bone mimetic anisotropic bone matrix microstructure from hiPSCs. Moreover, we demonstrated for the first time that the hiPSCs-derived osteoblasts possess a high level of intact functionality to regulate cell alignment. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 360–369, 2018.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	89833
citation_title	Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model
citation_author	Ozasa, Ryosuke
	Matsugaki, Aira
	Isobe, Yoshihiro
	Saku, Taro
	Yun, Hui Suk
	Nakano, Takayoshi
citation_publisher	John Wiley and Sons
citation_language	英語
citation_date	2017-09-16
citation_journal_title	Journal of Biomedical Materials Research - Part A
citation_volume	106
citation_issue	2
citation_firstpage	360
citation_lastpage	369
citation_doi	https://doi.org/10.1002/jbm.a.36238
citation_public_url	https://hdl.handle.net/11094/89833

Construction of human induced pluripotent stem cell-derived oriented bone matrix microstructure by using in vitro engineered anisotropic culture model

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