Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu

Hara, Emilio Satoshi; Okada, Masahiro; Kuboki, Takuo; Nakano, Takayoshi; Matsumoto, Takuya

doi:https://doi.org/10.1039/C8TB01544A

Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu

Hara, Emilio Satoshi; Okada, Masahiro; Kuboki, Takuo et al.

Journal of Materials Chemistry B, 2018, 6(38), 6153-6161

アクセス数:127件（2025-03-14 18:50 集計）

固定URL: https://hdl.handle.net/11094/89834

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タイトル	Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu
著者	NRID 1000040779443 1000040779443 著者名 Hara, Emilio Satoshi
	NRID 1000070416220 1000070416220 著者名 Okada, Masahiro
	NRID 1000000225195 1000000225195 著者名 Kuboki, Takuo
	NRID 1000030243182 1000030243182 ORCID 0000-0001-8052-1698 0000-0001-8052-1698 researchmap permalink read0013987 read0013987 Scopus Author ID 7403896013 7403896013 著者名 Nakano, Takayoshi
	NRID 1000040324793 1000040324793 著者名 Matsumoto, Takuya
内容	Hara E.S., Okada M., Kuboki T., et al. Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu. Journal of Materials Chemistry B, 6, 38, 6153. https://doi.org/10.1039/C8TB01544A.
抄録	Bone is a sophisticated organic-inorganic hybrid material, whose formation involves a complex spatio-temporal sequence of events regulated by the cells. A deeper understanding of the mechanisms behind bone mineralization at different size scales, and using a multidisciplinary approach, may uncover novel pathways for the design and fabrication of functional bone tissue in vitro. The objectives of this study were first to investigate the environmental factors that prime initial mineralization using the secondary ossification center as an in vivo model, and then to apply the obtained knowledge for rapid in vitro synthesis of bone-like tissue. First, the direct and robust measurement of pH showed that femur epiphysis is alkaline (pH ≅ 8.5) at the initial mineral stage at post-natal day 6. We showed that the alkaline milieu is decisive not only for alkaline phosphatase activity, which precedes mineral formation at P6, but also for determining initial mineral precipitation and spherical morphology. Next, engineering approaches were used to synthesize bone-like tissue based on alkaline milieu and artificial chondrocyte membrane nanofragments, previously shown to be the nucleation site for mineral formation. Interestingly, mineralization using artificial cell membrane nanofragments was achieved in just 1 day. Finally, ex vivo culture of femur epiphysis in alkaline pH strongly induced chondrocyte burst, which was previously shown to be the origin of chondrocyte membrane nanofragments, and also enhanced mineral formation. Taken together, these findings not only shed more light on the microenvironmental conditions that prime initial bone formation in vivo, but they also show that alkaline milieu can be used as an important factor for enhancing methods for in vitro synthesis of bone tissue.
出版者	Royal Society of Chemistry
収録物名	Journal of Materials Chemistry B
巻(号)	6(38)
ページ	6153-6161
刊行年月	2018-09-11
言語	英語
ハンドルURL	https://hdl.handle.net/11094/89834
PISSN	20507518
eISSN	2050750X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1039/C8TB01544A
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Materials Chemistry B. 2018, 6(38), p. 6153-6161
DC.title	Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu
DC.creator	Hara, Emilio Satoshi
	Okada, Masahiro
	Kuboki, Takuo
	Nakano, Takayoshi
	Matsumoto, Takuya
DC.publisher	Royal Society of Chemistry
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2018-09-11
DC.identifier	https://doi.org/10.1039/C8TB01544A
DC.description	Hara E.S., Okada M., Kuboki T., et al. Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu. Journal of Materials Chemistry B, 6, 38, 6153. https://doi.org/10.1039/C8TB01544A.
DCTERMS.abstract	Bone is a sophisticated organic-inorganic hybrid material, whose formation involves a complex spatio-temporal sequence of events regulated by the cells. A deeper understanding of the mechanisms behind bone mineralization at different size scales, and using a multidisciplinary approach, may uncover novel pathways for the design and fabrication of functional bone tissue in vitro. The objectives of this study were first to investigate the environmental factors that prime initial mineralization using the secondary ossification center as an in vivo model, and then to apply the obtained knowledge for rapid in vitro synthesis of bone-like tissue. First, the direct and robust measurement of pH showed that femur epiphysis is alkaline (pH ≅ 8.5) at the initial mineral stage at post-natal day 6. We showed that the alkaline milieu is decisive not only for alkaline phosphatase activity, which precedes mineral formation at P6, but also for determining initial mineral precipitation and spherical morphology. Next, engineering approaches were used to synthesize bone-like tissue based on alkaline milieu and artificial chondrocyte membrane nanofragments, previously shown to be the nucleation site for mineral formation. Interestingly, mineralization using artificial cell membrane nanofragments was achieved in just 1 day. Finally, ex vivo culture of femur epiphysis in alkaline pH strongly induced chondrocyte burst, which was previously shown to be the origin of chondrocyte membrane nanofragments, and also enhanced mineral formation. Taken together, these findings not only shed more light on the microenvironmental conditions that prime initial bone formation in vivo, but they also show that alkaline milieu can be used as an important factor for enhancing methods for in vitro synthesis of bone tissue.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 International License.
DC.format	89834
citation_title	Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu
citation_author	Hara, Emilio Satoshi
	Okada, Masahiro
	Kuboki, Takuo
	Nakano, Takayoshi
	Matsumoto, Takuya
citation_publisher	Royal Society of Chemistry
citation_language	英語
citation_date	2018-09-11
citation_journal_title	Journal of Materials Chemistry B
citation_volume	6
citation_issue	38
citation_firstpage	6153
citation_lastpage	6161
citation_doi	https://doi.org/10.1039/C8TB01544A
citation_public_url	https://hdl.handle.net/11094/89834

Rapid bioinspired mineralization using cell membrane nanofragments and alkaline milieu

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