Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast

Hirano, Yasuhiro; Kinugasa, Yasuha; Kubota, Yoshino; Obuse, Chikashi; Haraguchi, Tokuko; Hiraoka, Yasushi

doi:https://doi.org/10.1093/jb/mvad017

Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast

Hirano, Yasuhiro; Kinugasa, Yasuha; Kubota, Yoshino et al.

Journal of Biochemistry, 2023, 174(1), 33-46

アクセス数:380件（2025-06-19 10:53 集計）

固定URL: https://hdl.handle.net/11094/92448

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タイトル	Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast
著者	NRID 1000010508641 1000010508641 ORCID 0000-0001-7339-4053 0000-0001-7339-4053 researchmap permalink yasuhiro_hirano yasuhiro_hirano Scopus Author ID 57189010609 57189010609 著者名 Hirano, Yasuhiro
	NRID 1000060852118 1000060852118 著者名 Kinugasa, Yasuha
	researchmap permalink yoshinokubota yoshinokubota 著者名 Kubota, Yoshino
	NRID 1000000273855 1000000273855 researchmap permalink obuse obuse Scopus Author ID 6701730728 6701730728 著者名 Obuse, Chikashi
	NRID 1000020359079 1000020359079 ORCID 0000-0002-3813-6785 0000-0002-3813-6785 researchmap permalink haraguchitokuko haraguchitokuko Scopus Author ID 7103088325 7103088325 著者名 Haraguchi, Tokuko
	Scopus Author ID 7101710881 7101710881 著者名 Hiraoka, Yasushi
キーワード等	Bqt4
	Lem2
	nuclear envelope
	proteome analysis
	Schizosaccharomyces pombe
内容	This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record, Hirano Y., Kinugasa Y., Kubota Y., et al. Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast. 174(1), 33-46, (2023); is available online at: https://academic.oup.com/jb/article/174/1/33/7043862 and https://doi.org/10.1093/jb/mvad017
抄録	The nuclear envelope (NE) is a double-membrane structure consisting of inner and outer membranes that spatially separate the nucleus from the cytoplasm, and its function is critical for cellular functions such as genome maintenance. In the fission yeast, Schizosaccharomyces pombe, the inner nuclear membrane proteins, Lem2 and Bqt4, play pivotal roles in maintaining the NE structure. We previously found that the double deletion of lem2+ and bqt4+ causes a synthetic lethal defect associated with severe NE rupture, and overexpression of Elo2, a solo very-long-chain fatty acid elongase, suppresses this defect by restoring the NE. However, the molecular basis of this restoration remains elusive. To address this, we identified Lem2- and Bqt4-binding proteins via immunoprecipitation and mass spectrometry in this study. Forty-five and 23 proteins were identified as Lem2- and Bqt4-binding proteins, respectively. Although these binding proteins partially overlapped, Lem2 and Bqt4 interacted with different types of lipid metabolic enzymes: Cho2, Ole1 and Erg11 for Lem2 and Cwh43 for Bqt4. These enzymes are known to be involved in various lipid synthesis processes, suggesting that Lem2 and Bqt4 may contribute to the regulation of lipid synthesis by binding to these enzymes.
出版者	Oxford University Press
収録物名	Journal of Biochemistry
巻(号)	174(1)
ページ	33-46
刊行年月	2023-07
言語	英語
ハンドルURL	https://hdl.handle.net/11094/92448
PISSN	0021924X
eISSN	17562651
NCID	AA00694073
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1093/jb/mvad017
アクセス権	open access
権利情報	© 2023 Oxford University Press. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Journal of Biochemistry. 2023, 174(1), p. 33-46
DC.title	Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast
DC.creator	Hirano, Yasuhiro
	Kinugasa, Yasuha
	Kubota, Yoshino
	Obuse, Chikashi
	Haraguchi, Tokuko
	Hiraoka, Yasushi
DC.publisher	Oxford University Press
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-07
DC.identifier	https://doi.org/10.1093/jb/mvad017
DC.subject	Bqt4
	Lem2
	nuclear envelope
	proteome analysis
	Schizosaccharomyces pombe
DC.description	This is a pre-copyedited, author-produced version of an article accepted for publication in The Journal of Biochemistry following peer review. The version of record, Hirano Y., Kinugasa Y., Kubota Y., et al. Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast. 174(1), 33-46, (2023); is available online at: https://academic.oup.com/jb/article/174/1/33/7043862 and https://doi.org/10.1093/jb/mvad017
DCTERMS.abstract	The nuclear envelope (NE) is a double-membrane structure consisting of inner and outer membranes that spatially separate the nucleus from the cytoplasm, and its function is critical for cellular functions such as genome maintenance. In the fission yeast, Schizosaccharomyces pombe, the inner nuclear membrane proteins, Lem2 and Bqt4, play pivotal roles in maintaining the NE structure. We previously found that the double deletion of lem2+ and bqt4+ causes a synthetic lethal defect associated with severe NE rupture, and overexpression of Elo2, a solo very-long-chain fatty acid elongase, suppresses this defect by restoring the NE. However, the molecular basis of this restoration remains elusive. To address this, we identified Lem2- and Bqt4-binding proteins via immunoprecipitation and mass spectrometry in this study. Forty-five and 23 proteins were identified as Lem2- and Bqt4-binding proteins, respectively. Although these binding proteins partially overlapped, Lem2 and Bqt4 interacted with different types of lipid metabolic enzymes: Cho2, Ole1 and Erg11 for Lem2 and Cwh43 for Bqt4. These enzymes are known to be involved in various lipid synthesis processes, suggesting that Lem2 and Bqt4 may contribute to the regulation of lipid synthesis by binding to these enzymes.
DC.rights	© 2023 Oxford University Press. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
DC.format	92448
citation_title	Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast
citation_author	Hirano, Yasuhiro
	Kinugasa, Yasuha
	Kubota, Yoshino
	Obuse, Chikashi
	Haraguchi, Tokuko
	Hiraoka, Yasushi
citation_publisher	Oxford University Press
citation_language	英語
citation_date	2023-07
citation_journal_title	Journal of Biochemistry
citation_volume	174
citation_issue	1
citation_firstpage	33
citation_lastpage	46
citation_doi	https://doi.org/10.1093/jb/mvad017
citation_public_url	https://hdl.handle.net/11094/92448

Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast

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