Centromere/kinetochore is assembled through CENP-C oligomerization

Hara, Masatoshi; Ariyoshi, Mariko; Sano, Tomoki; Nozawa, Ryu-Suke; Shinkai, Soya; Onami, Shuichi; Jansen, Isabelle; Hirota, Toru; Fukagawa, Tatsuo

doi:https://doi.org/10.1016/j.molcel.2023.05.023

Centromere/kinetochore is assembled through CENP-C oligomerization

Hara, Masatoshi; Ariyoshi, Mariko; Sano, Tomoki et al.

Molecular Cell, 2023, 83(13), 2188-2205

アクセス数:804件（2025-09-29 21:32 集計）

固定URL: https://hdl.handle.net/11094/92473

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タイトル	Centromere/kinetochore is assembled through CENP-C oligomerization
著者	NRID 1000030565099 1000030565099 ORCID 0000-0001-8433-1111 0000-0001-8433-1111 researchmap permalink masatoshi_hara masatoshi_hara Scopus Author ID 56069726900 56069726900 著者名 Hara, Masatoshi
	NRID 1000080437243 1000080437243 ORCID 0000-0002-1361-2642 0000-0002-1361-2642 researchmap permalink 7000010594 7000010594 Scopus Author ID 7006547610 7006547610 著者名 Ariyoshi, Mariko
	著者名 Sano, Tomoki
	NRID 1000070868710 1000070868710 著者名 Nozawa, Ryu-Suke
	NRID 1000060547058 1000060547058 著者名 Shinkai, Soya
	NRID 1000050348843 1000050348843 著者名 Onami, Shuichi
	著者名 Jansen, Isabelle
	NRID 1000050421368 1000050421368 著者名 Hirota, Toru
	NRID 1000060321600 1000060321600 researchmap permalink fukagawa_tatsuo fukagawa_tatsuo Scopus Author ID 7003912496 7003912496 著者名 Fukagawa, Tatsuo
キーワード等	CCAN
	CENP-C
	centromere
	chromosome segregation
	kinetochore assembly
	oligomerization
内容	Hara M., Ariyoshi M., Sano T., et al. Centromere/kinetochore is assembled through CENP-C oligomerization. Molecular Cell 83, 2188 (2023); https://doi.org/10.1016/j.molcel.2023.05.023.
抄録	Kinetochore is an essential protein complex required for accurate chromosome segregation. The constitutive centromere-associated network (CCAN), a subcomplex of the kinetochore, associates with centromeric chromatin and provides a platform for the kinetochore assembly. The CCAN protein CENP-C is thought to be a central hub for the centromere/kinetochore organization. However, the role of CENP-C in CCAN assembly needs to be elucidated. Here, we demonstrate that both the CCAN-binding domain and the C-terminal region that includes the Cupin domain of CENP-C are necessary and sufficient for chicken CENP-C function. Structural and biochemical analyses reveal self-oligomerization of the Cupin domains of chicken and human CENP-C. We find that the CENP-C Cupin domain oligomerization is vital for CENP-C function, centromeric localization of CCAN, and centromeric chromatin organization. These results suggest that CENP-C facilitates the centromere/kinetochore assembly through its oligomerization.
出版者	Cell Press
収録物名	Molecular Cell
巻(号)	83(13)
ページ	2188-2205
刊行年月	2023-07-06
言語	英語
ハンドルURL	https://hdl.handle.net/11094/92473
PISSN	10972765
eISSN	10974164
NCID	AA1119005X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.molcel.2023.05.023
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Molecular Cell. 2023, 83(13), p. 2188-2205
DC.title	Centromere/kinetochore is assembled through CENP-C oligomerization
DC.creator	Hara, Masatoshi
	Ariyoshi, Mariko
	Sano, Tomoki
	Nozawa, Ryu-Suke
	Shinkai, Soya
	Onami, Shuichi
	Jansen, Isabelle
	Hirota, Toru
	Fukagawa, Tatsuo
DC.publisher	Cell Press
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-07-06
DC.identifier	https://doi.org/10.1016/j.molcel.2023.05.023
DC.subject	CCAN
	CENP-C
	centromere
	chromosome segregation
	kinetochore assembly
	oligomerization
DC.description	Hara M., Ariyoshi M., Sano T., et al. Centromere/kinetochore is assembled through CENP-C oligomerization. Molecular Cell 83, 2188 (2023); https://doi.org/10.1016/j.molcel.2023.05.023.
DCTERMS.abstract	Kinetochore is an essential protein complex required for accurate chromosome segregation. The constitutive centromere-associated network (CCAN), a subcomplex of the kinetochore, associates with centromeric chromatin and provides a platform for the kinetochore assembly. The CCAN protein CENP-C is thought to be a central hub for the centromere/kinetochore organization. However, the role of CENP-C in CCAN assembly needs to be elucidated. Here, we demonstrate that both the CCAN-binding domain and the C-terminal region that includes the Cupin domain of CENP-C are necessary and sufficient for chicken CENP-C function. Structural and biochemical analyses reveal self-oligomerization of the Cupin domains of chicken and human CENP-C. We find that the CENP-C Cupin domain oligomerization is vital for CENP-C function, centromeric localization of CCAN, and centromeric chromatin organization. These results suggest that CENP-C facilitates the centromere/kinetochore assembly through its oligomerization.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	92473
citation_title	Centromere/kinetochore is assembled through CENP-C oligomerization
citation_author	Hara, Masatoshi
	Ariyoshi, Mariko
	Sano, Tomoki
	Nozawa, Ryu-Suke
	Shinkai, Soya
	Onami, Shuichi
	Jansen, Isabelle
	Hirota, Toru
	Fukagawa, Tatsuo
citation_publisher	Cell Press
citation_language	英語
citation_date	2023-07-06
citation_journal_title	Molecular Cell
citation_volume	83
citation_issue	13
citation_firstpage	2188
citation_lastpage	2205
citation_doi	https://doi.org/10.1016/j.molcel.2023.05.023
citation_public_url	https://hdl.handle.net/11094/92473

Centromere/kinetochore is assembled through CENP-C oligomerization

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