Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere

Mongia Piyusha; Toyofuku, Naoko; Pan, Ziyi; Xu, Ran; Kinoshita, Yakumo; Oki, Keitaro; Takahashi, Hiroki; Ogura, Yoshitoshi; Hayashi, Tetsuya; Nakagawa, Takuro

doi:https://doi.org/10.1038/s42003-023-04925-9

Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere

Mongia Piyusha; Toyofuku, Naoko; Pan, Ziyi et al.

Communications Biology, 2023, 6, 551

アクセス数:967件（2025-07-27 05:04 集計）

固定URL: https://hdl.handle.net/11094/92490

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タイトル	Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere
著者	著者名 Mongia Piyusha
	著者名 Toyofuku, Naoko
	著者名 Pan, Ziyi
	著者名 Xu, Ran
	著者名 Kinoshita, Yakumo
	著者名 Oki, Keitaro
	NRID 1000060548460 1000060548460 著者名 Takahashi, Hiroki
	NRID 1000040363585 1000040363585 著者名 Ogura, Yoshitoshi
	NRID 1000010173014 1000010173014 著者名 Hayashi, Tetsuya
	NRID 1000020324866 1000020324866 ORCID 0000-0003-3455-8224 0000-0003-3455-8224 researchmap permalink read0068709 read0068709 Scopus Author ID 57202699267 57202699267 著者名 Nakagawa, Takuro
キーワード等	Centromeres
	DNA recombination
	Genomic instability
	Homologous recombination
内容	Mongia P., Toyofuku N., Pan Z., et al. Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere. Communications Biology 6, 551 (2023); https://doi.org/10.1038/s42003-023-04925-9.
抄録	Rad51 maintains genome integrity, whereas Rad52 causes non-canonical homologous recombination leading to gross chromosomal rearrangements (GCRs). Here we find that fission yeast Srr1/Ber1 and Skb1/PRMT5 promote GCRs at centromeres. Genetic and physical analyses show that srr1 and skb1 mutations reduce isochromosome formation mediated by centromere inverted repeats. srr1 increases DNA damage sensitivity in rad51 cells but does not abolish checkpoint response, suggesting that Srr1 promotes Rad51-independent DNA repair. srr1 and rad52 additively, while skb1 and rad52 epistatically reduce GCRs. Unlike srr1 or rad52, skb1 does not increase damage sensitivity. Skb1 regulates cell morphology and cell cycle with Slf1 and Pom1, respectively, but neither Slf1 nor Pom1 causes GCRs. Mutating conserved residues in the arginine methyltransferase domain of Skb1 greatly reduces GCRs. These results suggest that, through arginine methylation, Skb1 forms aberrant DNA structures leading to Rad52-dependent GCRs. This study has uncovered roles for Srr1 and Skb1 in GCRs at centromeres.
出版者	Nature Research
収録物名	Communications Biology
巻(号)	6
ページ	551
刊行年月	2023-05-26
言語	英語
ハンドルURL	https://hdl.handle.net/11094/92490
eISSN	23993642
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s42003-023-04925-9
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Communications Biology. 2023, 6, p. 551
DC.title	Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere
DC.creator	Mongia Piyusha
	Toyofuku, Naoko
	Pan, Ziyi
	Xu, Ran
	Kinoshita, Yakumo
	Oki, Keitaro
	Takahashi, Hiroki
	Ogura, Yoshitoshi
	Hayashi, Tetsuya
	Nakagawa, Takuro
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-05-26
DC.identifier	https://doi.org/10.1038/s42003-023-04925-9
DC.subject	Centromeres
	DNA recombination
	Genomic instability
	Homologous recombination
DC.description	Mongia P., Toyofuku N., Pan Z., et al. Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere. Communications Biology 6, 551 (2023); https://doi.org/10.1038/s42003-023-04925-9.
DCTERMS.abstract	Rad51 maintains genome integrity, whereas Rad52 causes non-canonical homologous recombination leading to gross chromosomal rearrangements (GCRs). Here we find that fission yeast Srr1/Ber1 and Skb1/PRMT5 promote GCRs at centromeres. Genetic and physical analyses show that srr1 and skb1 mutations reduce isochromosome formation mediated by centromere inverted repeats. srr1 increases DNA damage sensitivity in rad51 cells but does not abolish checkpoint response, suggesting that Srr1 promotes Rad51-independent DNA repair. srr1 and rad52 additively, while skb1 and rad52 epistatically reduce GCRs. Unlike srr1 or rad52, skb1 does not increase damage sensitivity. Skb1 regulates cell morphology and cell cycle with Slf1 and Pom1, respectively, but neither Slf1 nor Pom1 causes GCRs. Mutating conserved residues in the arginine methyltransferase domain of Skb1 greatly reduces GCRs. These results suggest that, through arginine methylation, Skb1 forms aberrant DNA structures leading to Rad52-dependent GCRs. This study has uncovered roles for Srr1 and Skb1 in GCRs at centromeres.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	92490
citation_title	Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere
citation_author	Mongia Piyusha
	Toyofuku, Naoko
	Pan, Ziyi
	Xu, Ran
	Kinoshita, Yakumo
	Oki, Keitaro
	Takahashi, Hiroki
	Ogura, Yoshitoshi
	Hayashi, Tetsuya
	Nakagawa, Takuro
citation_publisher	Nature Research
citation_language	英語
citation_date	2023-05-26
citation_journal_title	Communications Biology
citation_volume	6
citation_firstpage	551
citation_doi	https://doi.org/10.1038/s42003-023-04925-9
citation_public_url	https://hdl.handle.net/11094/92490

Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere

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