miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis

Iki, Taichiro; Kawaguchi, Shinichi; Kai, Toshie

Science Advances, 2023, 9(29), eadh0397

アクセス数:537件（2025-09-07 07:06 集計）

固定URL: https://hdl.handle.net/11094/92526

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タイトル
miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis
著者
NRID
1000020774011 1000020774011
ORCID
0000-0002-3215-4552 0000-0002-3215-4552
researchmap permalink
taichiroiki taichiroiki
Scopus Author ID
15049534100 15049534100
著者名
Iki, Taichiro
NRID
1000040321749 1000040321749
researchmap permalink
1234567890abc 1234567890abc
Scopus Author ID
36762092600 36762092600
著者名
Kawaguchi, Shinichi
NRID
1000040579786 1000040579786
ORCID
0000-0001-8675-8469 0000-0001-8675-8469
researchmap permalink
toshie_kai toshie_kai
Scopus Author ID
7102037941 7102037941
著者名
Kai, Toshie
内容
Iki T., Kawaguchi S., Kai T.. miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis. Science Advances 9, eadh0397 (2023); https://doi.org/10.1126/sciadv.adh0397.
抄録
PIWI-interacting RNA (piRNA) pathways control transposable elements (TEs) and endogenous genes, playing important roles in animal gamete formation. However, the underlying piRNA biogenesis mechanisms remain elusive. Here, we show that endogenous protein coding sequences (CDSs), which are normally used for translation, serve as origins of noncoding piRNA biogenesis in Drosophila melanogaster testes. The product, namely, CDS-piRNAs, formed silencing complexes with Aubergine (Aub) in germ cells. Proximity proteome and functional analyses show that CDS-piRNAs and cluster/TE-piRNAs are distinct species occupying Aub, the former loading selectively relies on chaperone Cyclophilin 40. Moreover, Argonaute 2 (Ago2) and Dicer-2 activities were found critical for CDS-piRNA production. We provide evidence that Ago2-bound short interfering RNAs (siRNAs) and microRNAs (miRNAs) specify precursors to be processed into piRNAs.We further demonstrate that Aub is crucial in spermatid differentiation, regulating chromatins through mRNA cleavage. Collectively, our data illustrate a unique strategy used by male germ line, expanding piRNA repertoire for silencing of endogenous genes during spermatogenesis.
出版者
American Association for the Advancement of Science
収録物名
Science Advances
巻(号)
9(29)
ページ
eadh0397
刊行年月
2023-07-21
言語
英語
ハンドルURL
https://hdl.handle.net/11094/92526
eISSN
23752548
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1126/sciadv.adh0397
アクセス権
open access
権利情報
© 2023 The Authors, some rights reserved. This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article