学術雑誌論文

学術雑誌論文

text

Scientific Reports. 2023, 13(1), p. 9228

Cigarette smoke extract impairs gingival epithelial barrier function

Nature Research

英語

text

2023-12-01

https://doi.org/10.1038/s41598-023-36366-z

Yamaga S., Tanigaki K., Nakamura E., et al. Cigarette smoke extract impairs gingival epithelial barrier function. Scientific Reports 13, 9228 (2023); https://doi.org/10.1038/s41598-023-36366-z.

We previously showed that junctional adhesion molecule 1 (JAM1) and coxsackievirus and adenovirus receptor (CXADR), tight junction-associated proteins, have important roles to maintain epithelial barrier function in gingival tissues. Smoking is considered to be a significant risk factor for periodontal disease. The present study was conducted to examine the effects of cigarette smoke extract (CSE) on JAM1 and CXADR in human gingival epithelial cells. CSE was found to cause translocation of JAM1 from the cellular surface to EGFR-positive endosomes, whereas CXADR did not. Using a three-dimensional multilayered gingival epithelial tissue model, CSE administration was found to increase permeability to lipopolysaccharide and peptidoglycan, whereas overexpression of JAM1 in the tissue model prevented penetration by those substrates. Furthermore, vitamin C increased JAM1 expression, and inhibited penetration of LPS and PGN induced by CSE. These findings strongly suggest that CSE disrupts gingival barrier function via dislocation of JAM1, thus allowing bacterial virulence factors to penetrate into subepithelial tissues. Furthermore, they indicate that vitamin C increases JAM1 expression and prevents disruption of gingival barrier function by CSE.

This article is licensed under a Creative Commons Attribution 4.0 International License.

92682

Cigarette smoke extract impairs gingival epithelial barrier function

Nature Research

英語

2023-12-01

Scientific Reports

13

1

9228

https://doi.org/10.1038/s41598-023-36366-z

https://hdl.handle.net/11094/92682