Imaging the bone-immune cell interaction in bone destruction

Hasegawa, Tetsuo; Kikuta, Junichi; Ishii, Masaru

doi:https://doi.org/10.3389/fimmu.2019.00596

アクセス数:35件（2024-08-26 21:16 集計）

固定URL: https://hdl.handle.net/11094/93172

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タイトル	Imaging the bone-immune cell interaction in bone destruction
著者	NRID 1000030773048 1000030773048 著者名 Hasegawa, Tetsuo
	NRID 1000060710069 1000060710069 researchmap permalink jkikuta jkikuta Scopus Author ID 36637603800 36637603800 著者名 Kikuta, Junichi
	NRID 1000010324758 1000010324758 researchmap permalink read0076684 read0076684 Scopus Author ID 35074093500 35074093500 著者名 Ishii, Masaru
キーワード等	Bone
	Cellular dynamics
	Intravital imaging
	Osteoclast
	PH probe
	Two-photon microscopy
内容	Hasegawa T., Kikuta J., Ishii M.. Imaging the bone-immune cell interaction in bone destruction. Frontiers in Immunology 10, 596 (2019); https://doi.org/10.3389/fimmu.2019.00596.
抄録	Bone is a highly dynamic organ that is continuously being remodeled by the reciprocal interactions between bone and immune cells. We have originally established an advanced imaging system for visualizing the in vivo behavior of osteoclasts and their precursors in the bone marrow cavity using two-photon microscopy. Using this system, we found that the blood-enriched lipid mediator, sphingosine-1-phosphate, controlled the migratory behavior of osteoclast precursors. We also developed pH-sensing chemical fluorescent probes to detect localized acidification by bone-resorbing osteoclasts on the bone surface in vivo, and identified two distinct functional states of differentiated osteoclasts, “bone-resorptive” and “non-resorptive.” Here, we summarize our studies on the dynamics and functions of bone and immune cells within the bone marrow. We further discuss how our intravital imaging techniques can be applied to evaluate the mechanisms of action of biological agents in inflammatory bone destruction. Our intravital imaging techniques would be beneficial for studying the cellular dynamics in arthritic inflammation and bone destruction in vivo and would also be useful for evaluating novel therapies in animal models of bone-destroying diseases.
出版者	Frontiers Media
収録物名	Frontiers in Immunology
巻(号)	10(MAR)
ページ	596
刊行年月	2019-03-26
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93172
eISSN	16643224
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.3389/fimmu.2019.00596
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Frontiers in Immunology. 2019, 10(MAR), p. 596
DC.title	Imaging the bone-immune cell interaction in bone destruction
DC.creator	Hasegawa, Tetsuo
	Kikuta, Junichi
	Ishii, Masaru
DC.publisher	Frontiers Media
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2019-03-26
DC.identifier	https://doi.org/10.3389/fimmu.2019.00596
DC.subject	Bone
	Cellular dynamics
	Intravital imaging
	Osteoclast
	PH probe
	Two-photon microscopy
DC.description	Hasegawa T., Kikuta J., Ishii M.. Imaging the bone-immune cell interaction in bone destruction. Frontiers in Immunology 10, 596 (2019); https://doi.org/10.3389/fimmu.2019.00596.
DCTERMS.abstract	Bone is a highly dynamic organ that is continuously being remodeled by the reciprocal interactions between bone and immune cells. We have originally established an advanced imaging system for visualizing the in vivo behavior of osteoclasts and their precursors in the bone marrow cavity using two-photon microscopy. Using this system, we found that the blood-enriched lipid mediator, sphingosine-1-phosphate, controlled the migratory behavior of osteoclast precursors. We also developed pH-sensing chemical fluorescent probes to detect localized acidification by bone-resorbing osteoclasts on the bone surface in vivo, and identified two distinct functional states of differentiated osteoclasts, “bone-resorptive” and “non-resorptive.” Here, we summarize our studies on the dynamics and functions of bone and immune cells within the bone marrow. We further discuss how our intravital imaging techniques can be applied to evaluate the mechanisms of action of biological agents in inflammatory bone destruction. Our intravital imaging techniques would be beneficial for studying the cellular dynamics in arthritic inflammation and bone destruction in vivo and would also be useful for evaluating novel therapies in animal models of bone-destroying diseases.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93172
citation_title	Imaging the bone-immune cell interaction in bone destruction
citation_author	Hasegawa, Tetsuo
	Kikuta, Junichi
	Ishii, Masaru
citation_publisher	Frontiers Media
citation_language	英語
citation_date	2019-03-26
citation_journal_title	Frontiers in Immunology
citation_volume	10
citation_issue	MAR
citation_firstpage	596
citation_doi	https://doi.org/10.3389/fimmu.2019.00596
citation_public_url	https://hdl.handle.net/11094/93172

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