A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis

Oyama, Shohei; Ebina, Kosuke; Etani, Yuki; Hirao, Makoto; Kyuuma, Masanao; Fujii, Yasuyuki; Iwata, Katsuya; Ogawa, Bunichiro; Hasegawa, Tomoya; Kawano, Sasagu; Nakanishi, Yutaka; Okada, Seiji; Nakata, Ken

doi:https://doi.org/10.1038/s41598-022-23152-6

A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis

Oyama, Shohei; Ebina, Kosuke; Etani, Yuki et al.

Scientific Reports, 2022, 12(1), 18102

アクセス数:1,382件（2025-07-17 14:24 集計）

固定URL: https://hdl.handle.net/11094/93205

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
SciRep_12_1_18102	pdf	なし	3.07 MB	111	2023.11.29

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis
著者	著者名 Oyama, Shohei
	NRID 1000070612076 1000070612076 researchmap permalink k-ebina k-ebina Scopus Author ID 8415453300 8415453300 ORCID 0000-0002-2426-1024 0000-0002-2426-1024 著者名 Ebina, Kosuke
	NRID 1000060898930 1000060898930 ORCID 0000-0001-5741-5969 0000-0001-5741-5969 researchmap permalink Yetani0302 Yetani0302 著者名 Etani, Yuki
	NRID 1000010527708 1000010527708 Scopus Author ID 8883255100 8883255100 著者名 Hirao, Makoto
	著者名 Kyuuma, Masanao
	著者名 Fujii, Yasuyuki
	著者名 Iwata, Katsuya
	著者名 Ogawa, Bunichiro
	著者名 Hasegawa, Tomoya
	著者名 Kawano, Sasagu
	著者名 Nakanishi, Yutaka
	NRID 1000030448435 1000030448435 ORCID 0000-0002-5107-8209 0000-0002-5107-8209 researchmap permalink okadaseiji okadaseiji Scopus Author ID 35079139100 35079139100 著者名 Okada, Seiji
	NRID 1000000283747 1000000283747 researchmap permalink ken-nakata ken-nakata Scopus Author ID 7402151308 7402151308 著者名 Nakata, Ken
内容	Oyama S., Ebina K., Etani Y., et al. A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis. Scientific Reports 12, 18102 (2022); https://doi.org/10.1038/s41598-022-23152-6.
抄録	In clinical studies, the next-generation anti-tumor necrosis factor-alpha (TNF-α) single domain antibody ozoralizumab showed high clinical efficacy shortly after the subcutaneous injection. To elucidate the mechanism underlying the rapid onset of the effects of ozoralizumab, we compared the biodistribution kinetics of ozoralizumab and adalimumab after subcutaneous injection in an animal model of arthritis. Alexa Fluor 680-labeled ozoralizumab and adalimumab were administered by subcutaneous injection once (2 mg/kg) at five weeks after induction of collagen-induced arthritis (CIA) in an animal arthritis model. The time-course of changes in the fluorescence intensities of the two compounds in the paws and serum were evaluated. The paws of the CIA mice were harvested at four and eight hours after the injection for fluorescence microscopy. Biofluorescence imaging revealed better distribution of ozoralizumab to the joint tissues than of adalimumab, as early as at four hours after the injection. Fluorescence microscopy revealed a greater fluorescence intensity of ozoralizumab in the joint tissues than that of adalimumab at eight hours after the injection. Ozoralizumab showed a significantly higher absorption rate constant as compared with adalimumab. These results indicate that ozoralizumab enters the systemic circulation more rapidly and is distributed to the target tissues earlier and at higher levels than conventional IgG antibodies. Our investigation provides new insight into the mechanism underlying the rapid onset of the effects of ozoralizumab in clinical practice.
出版者	Nature Research
収録物名	Scientific Reports
巻(号)	12(1)
ページ	18102
刊行年月	2022-10-27
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93205
eISSN	20452322
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41598-022-23152-6
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Scientific Reports. 2022, 12(1), p. 18102
DC.title	A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis
DC.creator	Oyama, Shohei
	Ebina, Kosuke
	Etani, Yuki
	Hirao, Makoto
	Kyuuma, Masanao
	Fujii, Yasuyuki
	Iwata, Katsuya
	Ogawa, Bunichiro
	Hasegawa, Tomoya
	Kawano, Sasagu
	Nakanishi, Yutaka
	Okada, Seiji
	Nakata, Ken
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-10-27
DC.identifier	https://doi.org/10.1038/s41598-022-23152-6
DC.description	Oyama S., Ebina K., Etani Y., et al. A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis. Scientific Reports 12, 18102 (2022); https://doi.org/10.1038/s41598-022-23152-6.
DCTERMS.abstract	In clinical studies, the next-generation anti-tumor necrosis factor-alpha (TNF-α) single domain antibody ozoralizumab showed high clinical efficacy shortly after the subcutaneous injection. To elucidate the mechanism underlying the rapid onset of the effects of ozoralizumab, we compared the biodistribution kinetics of ozoralizumab and adalimumab after subcutaneous injection in an animal model of arthritis. Alexa Fluor 680-labeled ozoralizumab and adalimumab were administered by subcutaneous injection once (2 mg/kg) at five weeks after induction of collagen-induced arthritis (CIA) in an animal arthritis model. The time-course of changes in the fluorescence intensities of the two compounds in the paws and serum were evaluated. The paws of the CIA mice were harvested at four and eight hours after the injection for fluorescence microscopy. Biofluorescence imaging revealed better distribution of ozoralizumab to the joint tissues than of adalimumab, as early as at four hours after the injection. Fluorescence microscopy revealed a greater fluorescence intensity of ozoralizumab in the joint tissues than that of adalimumab at eight hours after the injection. Ozoralizumab showed a significantly higher absorption rate constant as compared with adalimumab. These results indicate that ozoralizumab enters the systemic circulation more rapidly and is distributed to the target tissues earlier and at higher levels than conventional IgG antibodies. Our investigation provides new insight into the mechanism underlying the rapid onset of the effects of ozoralizumab in clinical practice.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93205
citation_title	A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis
citation_author	Oyama, Shohei
	Ebina, Kosuke
	Etani, Yuki
	Hirao, Makoto
	Kyuuma, Masanao
	Fujii, Yasuyuki
	Iwata, Katsuya
	Ogawa, Bunichiro
	Hasegawa, Tomoya
	Kawano, Sasagu
	Nakanishi, Yutaka
	Okada, Seiji
	Nakata, Ken
citation_publisher	Nature Research
citation_language	英語
citation_date	2022-10-27
citation_journal_title	Scientific Reports
citation_volume	12
citation_issue	1
citation_firstpage	18102
citation_doi	https://doi.org/10.1038/s41598-022-23152-6
citation_public_url	https://hdl.handle.net/11094/93205

A novel anti-TNF-α drug ozoralizumab rapidly distributes to inflamed joint tissues in a mouse model of collagen induced arthritis

閲覧可能ファイル

論文情報

論文詳細を表示