DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough

Hiramatsu, Yukihiro; Nishida, Takashi; Ota, Natsuko; Tamaki, Yuki; Nugraha, Dendi K.; Horiguchi, Yasuhiko

doi:https://doi.org/10.1073/pnas.2308260120

DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough

Hiramatsu, Yukihiro; Nishida, Takashi; Ota, Natsuko et al.

Proceedings of the National Academy of Sciences of the United States of America, 2023, 120(40), e2308260120

アクセス数:995件（2026-07-27 05:32 集計）

固定URL: https://hdl.handle.net/11094/93233

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
ProcNatlAcadSciUSA_120_40	pdf	なし	3.07 MB	171	2023.12.05

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough
著者	NRID 1000090739210 1000090739210 ORCID 0000-0002-6340-864X 0000-0002-6340-864X researchmap permalink yhiramatsu yhiramatsu Scopus Author ID 56603770000 56603770000 著者名 Hiramatsu, Yukihiro
	NRID 1000020845200 1000020845200 researchmap permalink tnisida tnisida Scopus Author ID 53064412900 53064412900 ORCID 0000-0003-4978-7716 0000-0003-4978-7716 著者名 Nishida, Takashi
	著者名 Ota, Natsuko
	著者名 Tamaki, Yuki
	著者名 Nugraha, Dendi K.
	NRID 1000000183939 1000000183939 ORCID 0000-0002-1592-5861 0000-0002-1592-5861 researchmap permalink read0014308 read0014308 Scopus Author ID 7102798909 7102798909 著者名 Horiguchi, Yasuhiko
キーワード等	Bordetella parapertussis
	cough
	DAT
	demyristoylation
	Gi GTPases
内容	Hiramatsu Y., Nishida T., Ota N., et al. DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough. Proceedings of the National Academy of Sciences of the United States of America 120, e2308260120 (2023); https://doi.org/10.1073/pnas.2308260120.
抄録	The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of the Gi family through ADP-ribosylation of their α subunits, causes coughing in combination with Vag8 and lipid A in B. pertussis infection. In contrast, the mechanism of cough induced by B. parapertussis, which produces Vag8 and lipopolysaccharide (LPS) containing lipid A, but not PTx, remained to be elucidated. Here, we show that a toxin we named deacylating autotransporter toxin (DAT) of B. parapertussis inactivates heterotrimeric Gi GTPases through demyristoylation of their α subunits and contributes to cough production along with Vag8 and LPS. These results indicate that DAT plays a role in B. parapertussis infection in place of PTx.
出版者	National Academy of Sciences
収録物名	Proceedings of the National Academy of Sciences of the United States of America
巻(号)	120(40)
ページ	e2308260120
刊行年月	2023-10-03
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93233
PISSN	00278424
eISSN	10916490
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1073/pnas.2308260120
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Proceedings of the National Academy of Sciences of the United States of America. 2023, 120(40), p. e2308260120
DC.title	DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough
DC.creator	Hiramatsu, Yukihiro
	Nishida, Takashi
	Ota, Natsuko
	Tamaki, Yuki
	Nugraha, Dendi K.
	Horiguchi, Yasuhiko
DC.publisher	National Academy of Sciences
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-10-03
DC.identifier	https://doi.org/10.1073/pnas.2308260120
DC.subject	Bordetella parapertussis
	cough
	DAT
	demyristoylation
	Gi GTPases
DC.description	Hiramatsu Y., Nishida T., Ota N., et al. DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough. Proceedings of the National Academy of Sciences of the United States of America 120, e2308260120 (2023); https://doi.org/10.1073/pnas.2308260120.
DCTERMS.abstract	The pathogenic bacteria Bordetella pertussis and Bordetella parapertussis cause pertussis (whooping cough) and pertussis-like disease, respectively, both of which are characterized by paroxysmal coughing. We previously reported that pertussis toxin (PTx), which inactivates heterotrimeric GTPases of the Gi family through ADP-ribosylation of their α subunits, causes coughing in combination with Vag8 and lipid A in B. pertussis infection. In contrast, the mechanism of cough induced by B. parapertussis, which produces Vag8 and lipopolysaccharide (LPS) containing lipid A, but not PTx, remained to be elucidated. Here, we show that a toxin we named deacylating autotransporter toxin (DAT) of B. parapertussis inactivates heterotrimeric Gi GTPases through demyristoylation of their α subunits and contributes to cough production along with Vag8 and LPS. These results indicate that DAT plays a role in B. parapertussis infection in place of PTx.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93233
citation_title	DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough
citation_author	Hiramatsu, Yukihiro
	Nishida, Takashi
	Ota, Natsuko
	Tamaki, Yuki
	Nugraha, Dendi K.
	Horiguchi, Yasuhiko
citation_publisher	National Academy of Sciences
citation_language	英語
citation_date	2023-10-03
citation_journal_title	Proceedings of the National Academy of Sciences of the United States of America
citation_volume	120
citation_issue	40
citation_firstpage	e2308260120
citation_doi	https://doi.org/10.1073/pnas.2308260120
citation_public_url	https://hdl.handle.net/11094/93233

DAT (deacylating autotransporter toxin) from Bordetella parapertussis demyristoylates Gαi GTPases and contributes to cough

閲覧可能ファイル

論文情報

論文詳細を表示