学術雑誌論文

学術雑誌論文

text

STAR Protocols. 2023, 4(3), p. 102471

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Cell Press

英語

text

2023-09-15

https://doi.org/10.1016/j.xpro.2023.102471

Matsuzaki T., Kawano Y., Horikiri M., et al. Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells. STAR Protocols 4, 102471 (2023); https://doi.org/10.1016/j.xpro.2023.102471.

Synthetic protocols providing mechanical patterns to culture substrate are essential to control the self-condensation of cells for organoid engineering. Here, we present a protocol for preparing hydrogels with mechanical patterns. We describe steps for hydrogel synthesis, mechanical evaluation of the substrate, and time-lapse imaging of cell self-organization. This protocol will facilitate the rational design of culture substrates with mechanical patterns for the engineering of various functional organoids. For complete details on the use and execution of this protocol, please refer to Takebe et al. (2015) and Matsuzaki et al. (2014, 2022).

This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

93286

Preparation of mechanically patterned hydrogels for controlling the self-condensation of cells

Cell Press

英語

2023-09-15

STAR Protocols

4

3

102471

https://doi.org/10.1016/j.xpro.2023.102471

https://hdl.handle.net/11094/93286