学術雑誌論文

学術雑誌論文

text

Journal of Physical Chemistry Letters. 2022, 13(40), p. 9494-9500

Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature

American Chemical Society

英語

text

2022-10-13

https://doi.org/10.1021/acs.jpclett.2c01673

Matsuzaki T., Terutsuki D., Sato S., et al. Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature. Journal of Physical Chemistry Letters 2022 13(40), 9494-9500. DOI: 10.1021/acs.jpclett.2c01673. Copyright © 2022 American Chemical Society.

Cell-coupled field-effect transistor (FET) biosensors have attracted considerable attention because of their high sensitivity to biomolecules. The use of insect cells (Sf21) as a core sensor element is advantageous due to their stable adhesion to sensors at room temperature. Although visualization of the insect cell-substrate interface leads to logical amplification of signals, the spatiotemporal processes at the interfaces have not yet been elucidated. We quantitatively monitored the adhesion dynamics of Sf21 using interference reflection microscopy (IRM). Specific adhesion signatures with ring-like patches along the cellular periphery were detected. A combination of zeta potential measurements and lectin staining identified specific glycoconjugates with low electrostatic potentials. The ring-like structures were disrupted after cholesterol depletion, suggesting a raft domain along the cell periphery. Our results indicate dynamic and asymmetric cell adhesion is due to low electrostatic repulsion with fluidic sugar rafts. We envision the logical design of cell-sensor interfaces with an electrical model that accounts for actual adhesion interfaces.

93288

Low Surface Potential with Glycoconjugates Determines Insect Cell Adhesion at Room Temperature

American Chemical Society

英語

2022-10-13

Journal of Physical Chemistry Letters

13

40

9494

9500

https://doi.org/10.1021/acs.jpclett.2c01673

https://hdl.handle.net/11094/93288