Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus

Kamatani, Takashi; Hagizawa, Hiroki; Yarimitsu, Seido; Morioka, Miho; Koyamatsu, Saeko; Sugimoto, Michihiko; Kodama, Joe; Yamane, Junko; Ishiguro, Hiroyuki; Shichino, Shigeyuki; Abe, Kuniya; Fujibuchi, Wataru; Fujie, Hiromichi; Kaito, Takashi; Tsumaki, Noriyuki

doi:https://doi.org/10.1016/j.biomaterials.2022.121491

Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus

Kamatani, Takashi; Hagizawa, Hiroki; Yarimitsu, Seido et al.

Biomaterials, 2022, 284, 121491

アクセス数:971件（2025-07-18 10:18 集計）

固定URL: https://hdl.handle.net/11094/93297

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
Biomaterials_284_121491	pdf	なし	11.6 MB	103	2023.12.12

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus
著者	著者名 Kamatani, Takashi
	著者名 Hagizawa, Hiroki
	NRID 1000090723205 1000090723205 著者名 Yarimitsu, Seido
	著者名 Morioka, Miho
	著者名 Koyamatsu, Saeko
	NRID 1000010373317 1000010373317 著者名 Sugimoto, Michihiko
	著者名 Kodama, Joe
	NRID 1000010433278 1000010433278 著者名 Yamane, Junko
	NRID 1000010433278 1000010433278 著者名 Ishiguro, Hiroyuki
	NRID 1000070822435 1000070822435 著者名 Shichino, Shigeyuki
	NRID 1000040240915 1000040240915 著者名 Abe, Kuniya
	NRID 1000060273512 1000060273512 著者名 Fujibuchi, Wataru
	NRID 1000020199300 1000020199300 著者名 Fujie, Hiromichi
	NRID 1000070623982 1000070623982 ORCID 0000-0003-4882-2997 0000-0003-4882-2997 researchmap permalink 911_0 911_0 Scopus Author ID 25824322100 25824322100 著者名 Kaito, Takashi
	NRID 1000050303938 1000050303938 ORCID 0000-0002-0520-3654 0000-0002-0520-3654 researchmap permalink cart cart Scopus Author ID 7004069586 7004069586 著者名 Tsumaki, Noriyuki
キーワード等	Cartilage
	Chondrocytes
	Induced pluripotent stem cells
	intervertebral disc
	Nucleus pulposus
	Regeneration
内容	Kamatani T., Hagizawa H., Yarimitsu S., et al. Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus. Biomaterials 284, 121491 (2022); https://doi.org/10.1016/j.biomaterials.2022.121491.
抄録	The loss of nucleus pulposus (NP) precedes the intervertebral disk (IVD) degeneration that causes back pain. Here, we demonstrate that the implantation of human iPS cell-derived cartilaginous tissue (hiPS-Cart) restores this loss by replacing lost NP spatially and functionally. NP cells consist of notochordal NP cells and chondrocyte-like NP cells. Single cell RNA sequencing (scRNA-seq) analysis revealed that cells in hiPS-Cart corresponded to chondrocyte-like NP cells but not to notochordal NP cells. The implantation of hiPS-Cart into a nuclectomized space of IVD in nude rats prevented the degeneration of the IVD and preserved its mechanical properties. hiPS-Cart survived and occupied the nuclectomized space for at least six months after implantation, indicating spatial and functional replacement of lost NP by hiPS-Cart. Further scRNA-seq analysis revealed that hiPS-Cart cells changed their profile after implantation, differentiating into two lineages that are metabolically distinct from each other. However, post-implanted hiPS-Cart cells corresponded to chondrocyte-like NP cells only and did not develop into notochordal NP cells, suggesting that chondrocyte-like NP cells are nearly sufficient for NP function. The data collectively indicate that hiPS-Cart is a candidate implant for regenerating NP spatially and functionally and preventing IVD degeneration.
出版者	Elsevier Ltd
収録物名	Biomaterials
巻(号)	284
ページ	121491
刊行年月	2022-05-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93297
PISSN	01429612
eISSN	18785905
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.biomaterials.2022.121491
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biomaterials. 2022, 284, p. 121491
DC.title	Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus
DC.creator	Kamatani, Takashi
	Hagizawa, Hiroki
	Yarimitsu, Seido
	Morioka, Miho
	Koyamatsu, Saeko
	Sugimoto, Michihiko
	Kodama, Joe
	Yamane, Junko
	Ishiguro, Hiroyuki
	Shichino, Shigeyuki
	Abe, Kuniya
	Fujibuchi, Wataru
	Fujie, Hiromichi
	Kaito, Takashi
	Tsumaki, Noriyuki
DC.publisher	Elsevier Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2022-05-01
DC.identifier	https://doi.org/10.1016/j.biomaterials.2022.121491
DC.subject	Cartilage
	Chondrocytes
	Induced pluripotent stem cells
	intervertebral disc
	Nucleus pulposus
	Regeneration
DC.description	Kamatani T., Hagizawa H., Yarimitsu S., et al. Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus. Biomaterials 284, 121491 (2022); https://doi.org/10.1016/j.biomaterials.2022.121491.
DCTERMS.abstract	The loss of nucleus pulposus (NP) precedes the intervertebral disk (IVD) degeneration that causes back pain. Here, we demonstrate that the implantation of human iPS cell-derived cartilaginous tissue (hiPS-Cart) restores this loss by replacing lost NP spatially and functionally. NP cells consist of notochordal NP cells and chondrocyte-like NP cells. Single cell RNA sequencing (scRNA-seq) analysis revealed that cells in hiPS-Cart corresponded to chondrocyte-like NP cells but not to notochordal NP cells. The implantation of hiPS-Cart into a nuclectomized space of IVD in nude rats prevented the degeneration of the IVD and preserved its mechanical properties. hiPS-Cart survived and occupied the nuclectomized space for at least six months after implantation, indicating spatial and functional replacement of lost NP by hiPS-Cart. Further scRNA-seq analysis revealed that hiPS-Cart cells changed their profile after implantation, differentiating into two lineages that are metabolically distinct from each other. However, post-implanted hiPS-Cart cells corresponded to chondrocyte-like NP cells only and did not develop into notochordal NP cells, suggesting that chondrocyte-like NP cells are nearly sufficient for NP function. The data collectively indicate that hiPS-Cart is a candidate implant for regenerating NP spatially and functionally and preventing IVD degeneration.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93297
citation_title	Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus
citation_author	Kamatani, Takashi
	Hagizawa, Hiroki
	Yarimitsu, Seido
	Morioka, Miho
	Koyamatsu, Saeko
	Sugimoto, Michihiko
	Kodama, Joe
	Yamane, Junko
	Ishiguro, Hiroyuki
	Shichino, Shigeyuki
	Abe, Kuniya
	Fujibuchi, Wataru
	Fujie, Hiromichi
	Kaito, Takashi
	Tsumaki, Noriyuki
citation_publisher	Elsevier Ltd
citation_language	英語
citation_date	2022-05-01
citation_journal_title	Biomaterials
citation_volume	284
citation_firstpage	121491
citation_doi	https://doi.org/10.1016/j.biomaterials.2022.121491
citation_public_url	https://hdl.handle.net/11094/93297

Human iPS cell-derived cartilaginous tissue spatially and functionally replaces nucleus pulposus

閲覧可能ファイル

論文情報

論文詳細を表示