Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage

Chen, Xike; Yamashita, Akihiro; Morioka, Miho; Senba, Toshika; Kamatani, Takashi; Watanabe, Akira; Kosai, Azuma; Tsumaki, Noriyuki

doi:https://doi.org/10.1089/ten.tea.2018.0133

Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage

Chen, Xike; Yamashita, Akihiro; Morioka, Miho et al.

Tissue Engineering - Part A, 2019, 25(5-6), 437-445

アクセス数:806件（2025-08-14 20:04 集計）

固定URL: https://hdl.handle.net/11094/93306

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タイトル	Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage
著者	著者名 Chen, Xike
	NRID 1000000636855 1000000636855 researchmap permalink Aki_yama26 Aki_yama26 Scopus Author ID 56404044900 56404044900 著者名 Yamashita, Akihiro
	著者名 Morioka, Miho
	著者名 Senba, Toshika
	NRID 1000090645764 1000090645764 著者名 Kamatani, Takashi
	NRID 1000060506765 1000060506765 著者名 Watanabe, Akira
	著者名 Kosai, Azuma
	NRID 1000050303938 1000050303938 ORCID 0000-0002-0520-3654 0000-0002-0520-3654 researchmap permalink cart cart Scopus Author ID 7004069586 7004069586 著者名 Tsumaki, Noriyuki
キーワード等	cartilage
	chondrocyte
	FGF
	iPS cells
	perichondrium
内容	Chen X., Yamashita A., Morioka M., et al. Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage. Tissue Engineering - Part A 25, 437 (2019); https://doi.org/10.1089/ten.tea.2018.0133.
抄録	New cell and tissue sources are needed for the regenerative treatment of articular cartilage damage. Human induced pluripotent stem cells (hiPSCs) are an abundant cell source due to their self-renewal capacity. Hyaline cartilage tissue particles derived from hiPSCs (hiPS-Carts), 1–3 mm in diameter, are one candidate source that can be used for transplantation. When transplanted to fill the defects of articular cartilage, hiPS-Carts form a repair tissue by integrating with each other and with adjacent host tissue. In this study, we analyzed the integration capacity using an in vitro model and found that hiPS-Carts spontaneously integrate with each other in vitro. hiPS-Carts consist of cartilage at the center and perichondrium-like membrane that wraps around the cartilage. The integration started at the perichondrium-like membrane at around 1 week. Then, the integration progressed to the cartilage within 4–8 weeks. RNA sequencing analysis identified a higher expression of FGF18 in the perichondrium-like membrane in hiPS-Carts compared with the central cartilage. The addition of FGF18 to the model accelerated the integration of hiPS-Carts, whereas the addition of a FGFR inhibitor inhibited it. These results suggest that FGF18 secreted from the perichondrium-like membrane plays a role in the integration of hiPS-Carts. Understanding the integration mechanism of hiPS-Carts is expected to contribute to the realization of regenerative treatment for patients with articular cartilage damage.
出版者	Mary Ann Liebert Inc.
収録物名	Tissue Engineering - Part A
巻(号)	25(5-6)
ページ	437-445
刊行年月	2019-04-17
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93306
PISSN	19373341
eISSN	1937335X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1089/ten.tea.2018.0133
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Tissue Engineering - Part A. 2019, 25(5-6), p. 437-445
DC.title	Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage
DC.creator	Chen, Xike
	Yamashita, Akihiro
	Morioka, Miho
	Senba, Toshika
	Kamatani, Takashi
	Watanabe, Akira
	Kosai, Azuma
	Tsumaki, Noriyuki
DC.publisher	Mary Ann Liebert Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2019-04-17
DC.identifier	https://doi.org/10.1089/ten.tea.2018.0133
DC.subject	cartilage
	chondrocyte
	FGF
	iPS cells
	perichondrium
DC.description	Chen X., Yamashita A., Morioka M., et al. Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage. Tissue Engineering - Part A 25, 437 (2019); https://doi.org/10.1089/ten.tea.2018.0133.
DCTERMS.abstract	New cell and tissue sources are needed for the regenerative treatment of articular cartilage damage. Human induced pluripotent stem cells (hiPSCs) are an abundant cell source due to their self-renewal capacity. Hyaline cartilage tissue particles derived from hiPSCs (hiPS-Carts), 1–3 mm in diameter, are one candidate source that can be used for transplantation. When transplanted to fill the defects of articular cartilage, hiPS-Carts form a repair tissue by integrating with each other and with adjacent host tissue. In this study, we analyzed the integration capacity using an in vitro model and found that hiPS-Carts spontaneously integrate with each other in vitro. hiPS-Carts consist of cartilage at the center and perichondrium-like membrane that wraps around the cartilage. The integration started at the perichondrium-like membrane at around 1 week. Then, the integration progressed to the cartilage within 4–8 weeks. RNA sequencing analysis identified a higher expression of FGF18 in the perichondrium-like membrane in hiPS-Carts compared with the central cartilage. The addition of FGF18 to the model accelerated the integration of hiPS-Carts, whereas the addition of a FGFR inhibitor inhibited it. These results suggest that FGF18 secreted from the perichondrium-like membrane plays a role in the integration of hiPS-Carts. Understanding the integration mechanism of hiPS-Carts is expected to contribute to the realization of regenerative treatment for patients with articular cartilage damage.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
DC.format	93306
citation_title	Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage
citation_author	Chen, Xike
	Yamashita, Akihiro
	Morioka, Miho
	Senba, Toshika
	Kamatani, Takashi
	Watanabe, Akira
	Kosai, Azuma
	Tsumaki, Noriyuki
citation_publisher	Mary Ann Liebert Inc.
citation_language	英語
citation_date	2019-04-17
citation_journal_title	Tissue Engineering - Part A
citation_volume	25
citation_issue	5-6
citation_firstpage	437
citation_lastpage	445
citation_doi	https://doi.org/10.1089/ten.tea.2018.0133
citation_public_url	https://hdl.handle.net/11094/93306

Integration Capacity of Human Induced Pluripotent Stem Cell-Derived Cartilage

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