FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination

Ito, Masaru; Furukohri, Asako; Matsuzaki, Kenichiro; Fujita, Yurika; Toyoda, Atsushi; Shinohara, Akira

doi:https://doi.org/10.1038/s41467-023-42576-w

FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination

Ito, Masaru; Furukohri, Asako; Matsuzaki, Kenichiro et al.

Nature Communications, 2023, 14, 6857

アクセス数:828件（2025-08-14 17:31 集計）

固定URL: https://hdl.handle.net/11094/93324

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タイトル	FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination
著者	NRID 1000030869061 1000030869061 researchmap permalink msrito msrito Scopus Author ID 35278000500 35278000500 著者名 Ito, Masaru
	NRID 1000090546293 1000090546293 researchmap permalink afurukohri afurukohri Scopus Author ID 25228605000 25228605000 著者名 Furukohri, Asako
	NRID 1000010772147 1000010772147 著者名 Matsuzaki, Kenichiro
	NRID 1000050893913 1000050893913 ORCID 0000-0002-4350-7229 0000-0002-4350-7229 researchmap permalink yurikafujita yurikafujita Scopus Author ID 57146062800 57146062800 著者名 Fujita, Yurika
	NRID 1000010267495 1000010267495 著者名 Toyoda, Atsushi
	NRID 1000000252578 1000000252578 ORCID 0000-0003-4207-8247 0000-0003-4207-8247 researchmap permalink akirashinohara13 akirashinohara13 Scopus Author ID 7102342573 7102342573 著者名 Shinohara, Akira
内容	Ito M., Furukohri A., Matsuzaki K., et al. FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination. Nature Communications 14, 6857 (2023); https://doi.org/10.1038/s41467-023-42576-w.
抄録	The formation of RAD51/DMC1 filaments on single-stranded (ss)DNAs essential for homology search and strand exchange in DNA double-strand break (DSB) repair is tightly regulated. FIGNL1 AAA+++ ATPase controls RAD51-mediated recombination in human cells. However, its role in gametogenesis remains unsolved. Here, we characterized a germ line-specific conditional knockout (cKO) mouse of FIGNL1. Fignl1 cKO male mice showed defective chromosome synapsis and impaired meiotic DSB repair with the accumulation of RAD51/DMC1 on meiotic chromosomes, supporting a positive role of FIGNL1 in homologous recombination at a post-assembly stage of RAD51/DMC1 filaments. Fignl1 cKO spermatocytes also accumulate RAD51/DMC1 on chromosomes in pre-meiotic S-phase. These RAD51/DMC1 assemblies are independent of meiotic DSB formation. We also showed that purified FIGNL1 dismantles RAD51 filament on double-stranded (ds)DNA as well as ssDNA. These results suggest an additional role of FIGNL1 in limiting the non-productive assembly of RAD51/DMC1 on native dsDNAs during pre-meiotic S-phase and meiotic prophase I.
出版者	Nature Research
収録物名	Nature Communications
巻(号)	14
ページ	6857
刊行年月	2023-10-27
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93324
eISSN	20411723
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41467-023-42576-w
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Nature Communications. 2023, 14, p. 6857
DC.title	FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination
DC.creator	Ito, Masaru
	Furukohri, Asako
	Matsuzaki, Kenichiro
	Fujita, Yurika
	Toyoda, Atsushi
	Shinohara, Akira
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-10-27
DC.identifier	https://doi.org/10.1038/s41467-023-42576-w
DC.description	Ito M., Furukohri A., Matsuzaki K., et al. FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination. Nature Communications 14, 6857 (2023); https://doi.org/10.1038/s41467-023-42576-w.
DCTERMS.abstract	The formation of RAD51/DMC1 filaments on single-stranded (ss)DNAs essential for homology search and strand exchange in DNA double-strand break (DSB) repair is tightly regulated. FIGNL1 AAA+++ ATPase controls RAD51-mediated recombination in human cells. However, its role in gametogenesis remains unsolved. Here, we characterized a germ line-specific conditional knockout (cKO) mouse of FIGNL1. Fignl1 cKO male mice showed defective chromosome synapsis and impaired meiotic DSB repair with the accumulation of RAD51/DMC1 on meiotic chromosomes, supporting a positive role of FIGNL1 in homologous recombination at a post-assembly stage of RAD51/DMC1 filaments. Fignl1 cKO spermatocytes also accumulate RAD51/DMC1 on chromosomes in pre-meiotic S-phase. These RAD51/DMC1 assemblies are independent of meiotic DSB formation. We also showed that purified FIGNL1 dismantles RAD51 filament on double-stranded (ds)DNA as well as ssDNA. These results suggest an additional role of FIGNL1 in limiting the non-productive assembly of RAD51/DMC1 on native dsDNAs during pre-meiotic S-phase and meiotic prophase I.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93324
citation_title	FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination
citation_author	Ito, Masaru
	Furukohri, Asako
	Matsuzaki, Kenichiro
	Fujita, Yurika
	Toyoda, Atsushi
	Shinohara, Akira
citation_publisher	Nature Research
citation_language	英語
citation_date	2023-10-27
citation_journal_title	Nature Communications
citation_volume	14
citation_firstpage	6857
citation_doi	https://doi.org/10.1038/s41467-023-42576-w
citation_public_url	https://hdl.handle.net/11094/93324

FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination

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