Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus

Istiandari, Pramesti; Yasumoto, Shuhei; Seki, Hikaru; Fukushima, Ery Odette; Muranaka, Toshiya

doi:https://doi.org/10.3389/fpls.2023.1214602

Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus

Istiandari, Pramesti; Yasumoto, Shuhei; Seki, Hikaru et al.

Frontiers in Plant Science, 2023, 14, 1214602

アクセス数:526件（2025-08-14 16:51 集計）

固定URL: https://hdl.handle.net/11094/93371

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タイトル	Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus
著者	著者名 Istiandari, Pramesti
	NRID 1000080853142 1000080853142 researchmap permalink yasumotoshuhei yasumotoshuhei Scopus Author ID 56395055300 56395055300 著者名 Yasumoto, Shuhei
	NRID 1000030392004 1000030392004 ORCID 0000-0002-6426-0368 0000-0002-6426-0368 researchmap permalink read0152753 read0152753 Scopus Author ID 7202492827 7202492827 著者名 Seki, Hikaru
	著者名 Fukushima, Ery Odette
	NRID 1000060342862 1000060342862 ORCID 0000-0003-1058-2473 0000-0003-1058-2473 researchmap permalink bio bio Scopus Author ID 7006488017 7006488017 著者名 Muranaka, Toshiya
キーワード等	CRISPR/Cas9
	cytochrome P450 monooxygenases (CYP)
	LORE1
	Lotus japonicus
	NADPH-cytochrome P450 reductases (CPR)
	triterpenoid biosynthesis
内容	Istiandari P., Yasumoto S., Seki H., et al. Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus. Frontiers in Plant Science 14, 1214602 (2023); https://doi.org/10.3389/fpls.2023.1214602.
抄録	Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the structural diversification of triterpenoids. To perform site-specific oxidations of the triterpene scaffold, CYPs require electrons transferred by NADPH-cytochrome P450 reductase (CPR), which is classified into two main classes, class I and class II, based on their structural difference. Lotus japonicus is a triterpenoids-producing model legume with one CPR class I gene (LjCPR1) and a minimum of two CPR class II genes (LjCPR2-1 and LjCPR2-2). CPR classes I and II from different plants have been reported to be involved in different metabolic pathways. By performing gene expression analyses of L. japonicus hairy root culture treated with methyl jasmonate (MeJA), this study revealed that LjCPR1, CYP716A51, and LUS were down-regulated which resulted in no change in betulinic acid and lupeol content. In contrast, LjCPR2s, bAS, CYP93E1, and CYP72A61 were significantly upregulated by MeJA treatment, followed by a significant increase of the precursors for soyasaponins, i.e. β-amyrin, 24-OH β-amyrin, and sophoradiol content. Triterpenoids profile analysis of LORE1 insertion and hairy root mutants showed that the loss of the Ljcpr2-1 gene significantly reduced soyasaponins precursors but not in Ljcpr1 mutants. However, Ljcpr1 and Ljcpr2-1 mutants showed a significant reduction in lupeol and oleanolic, ursolic, and betulinic acid contents. Furthermore, LjCPR1, but not LjCPR2, was crucial for seed development, supporting the previous notion that CPR class I might support plant basal metabolism. This study suggests that CPR classes I and II play different roles in L. japonicus triterpenoid biosynthesis.
出版者	Frontiers Media SA
収録物名	Frontiers in Plant Science
巻(号)	14
ページ	1214602
刊行年月	2023-08-09
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93371
eISSN	1664462X
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.3389/fpls.2023.1214602
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Frontiers in Plant Science. 2023, 14, p. 1214602
DC.title	Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus
DC.creator	Istiandari, Pramesti
	Yasumoto, Shuhei
	Seki, Hikaru
	Fukushima, Ery Odette
	Muranaka, Toshiya
DC.publisher	Frontiers Media SA
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-08-09
DC.identifier	https://doi.org/10.3389/fpls.2023.1214602
DC.subject	CRISPR/Cas9
	cytochrome P450 monooxygenases (CYP)
	LORE1
	Lotus japonicus
	NADPH-cytochrome P450 reductases (CPR)
	triterpenoid biosynthesis
DC.description	Istiandari P., Yasumoto S., Seki H., et al. Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus. Frontiers in Plant Science 14, 1214602 (2023); https://doi.org/10.3389/fpls.2023.1214602.
DCTERMS.abstract	Cytochrome P450 monooxygenases (CYPs) are enzymes that play critical roles in the structural diversification of triterpenoids. To perform site-specific oxidations of the triterpene scaffold, CYPs require electrons transferred by NADPH-cytochrome P450 reductase (CPR), which is classified into two main classes, class I and class II, based on their structural difference. Lotus japonicus is a triterpenoids-producing model legume with one CPR class I gene (LjCPR1) and a minimum of two CPR class II genes (LjCPR2-1 and LjCPR2-2). CPR classes I and II from different plants have been reported to be involved in different metabolic pathways. By performing gene expression analyses of L. japonicus hairy root culture treated with methyl jasmonate (MeJA), this study revealed that LjCPR1, CYP716A51, and LUS were down-regulated which resulted in no change in betulinic acid and lupeol content. In contrast, LjCPR2s, bAS, CYP93E1, and CYP72A61 were significantly upregulated by MeJA treatment, followed by a significant increase of the precursors for soyasaponins, i.e. β-amyrin, 24-OH β-amyrin, and sophoradiol content. Triterpenoids profile analysis of LORE1 insertion and hairy root mutants showed that the loss of the Ljcpr2-1 gene significantly reduced soyasaponins precursors but not in Ljcpr1 mutants. However, Ljcpr1 and Ljcpr2-1 mutants showed a significant reduction in lupeol and oleanolic, ursolic, and betulinic acid contents. Furthermore, LjCPR1, but not LjCPR2, was crucial for seed development, supporting the previous notion that CPR class I might support plant basal metabolism. This study suggests that CPR classes I and II play different roles in L. japonicus triterpenoid biosynthesis.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93371
citation_title	Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus
citation_author	Istiandari, Pramesti
	Yasumoto, Shuhei
	Seki, Hikaru
	Fukushima, Ery Odette
	Muranaka, Toshiya
citation_publisher	Frontiers Media SA
citation_language	英語
citation_date	2023-08-09
citation_journal_title	Frontiers in Plant Science
citation_volume	14
citation_firstpage	1214602
citation_doi	https://doi.org/10.3389/fpls.2023.1214602
citation_public_url	https://hdl.handle.net/11094/93371

Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus

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