Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB

Zou, Juqi; Anai, Satoshi; Ota, Satoshi; Ishitani, Shizuka; Oginuma, Masayuki; Ishitani, Tohru

doi:https://doi.org/10.1038/s41467-023-42963-3

Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB

Zou, Juqi; Anai, Satoshi; Ota, Satoshi et al.

Nature Communications, 2023, 14, 7194

アクセス数:519件（2025-07-22 20:50 集計）

固定URL: https://hdl.handle.net/11094/93372

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タイトル	Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB
著者	著者名 Zou, Juqi
	著者名 Anai, Satoshi
	NRID 1000060553310 1000060553310 著者名 Ota, Satoshi
	NRID 1000090608861 1000090608861 researchmap permalink shizukaishitani shizukaishitani Scopus Author ID 35310617400 35310617400 著者名 Ishitani, Shizuka
	NRID 1000050825966 1000050825966 researchmap permalink 7000024715 7000024715 Scopus Author ID 16040149700 16040149700 著者名 Oginuma, Masayuki
	NRID 1000040448428 1000040448428 ORCID 0000-0002-6200-7043 0000-0002-6200-7043 researchmap permalink read0148831 read0148831 Scopus Author ID 8079317200 8079317200 著者名 Ishitani, Tohru
内容	Zou J., Anai S., Ota S., et al. Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB. Nature Communications 14, 7194 (2023); https://doi.org/10.1038/s41467-023-42963-3.
抄録	In vertebrate embryos, the canonical Wnt ligand primes the formation of dorsal organizers that govern dorsal-ventral patterns by secreting BMP antagonists. In contrast, in Drosophila embryos, Toll-like receptor (Tlr)-mediated NFκB activation initiates dorsal-ventral patterning, wherein Wnt-mediated negative feedback regulation of Tlr/NFκB generates a BMP antagonist-secreting signalling centre to control the dorsal-ventral pattern. Although both Wnt and BMP antagonist are conserved among species, the involvement of Tlr/NFκB and feedback regulation in vertebrate organizer formation remains unclear. By imaging and genetic modification, we reveal that a negative feedback loop between canonical and non-canonical Wnts and Tlr4/NFκB determines the size of zebrafish organizer, and that Tlr/NFκB and Wnts switch initial cue and feedback mediator roles between Drosophila and zebrafish. Here, we show that canonical Wnt signalling stimulates the expression of the non-canonical Wnt5b ligand, activating the Tlr4 receptor to stimulate NFκB-mediated transcription of the Wnt antagonist frzb, restricting Wnt-dependent dorsal organizer formation.
出版者	Nature Research
収録物名	Nature Communications
巻(号)	14
ページ	7194
刊行年月	2023-11-08
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93372
eISSN	20411723
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1038/s41467-023-42963-3
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Nature Communications. 2023, 14, p. 7194
DC.title	Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB
DC.creator	Zou, Juqi
	Anai, Satoshi
	Ota, Satoshi
	Ishitani, Shizuka
	Oginuma, Masayuki
	Ishitani, Tohru
DC.publisher	Nature Research
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-11-08
DC.identifier	https://doi.org/10.1038/s41467-023-42963-3
DC.description	Zou J., Anai S., Ota S., et al. Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB. Nature Communications 14, 7194 (2023); https://doi.org/10.1038/s41467-023-42963-3.
DCTERMS.abstract	In vertebrate embryos, the canonical Wnt ligand primes the formation of dorsal organizers that govern dorsal-ventral patterns by secreting BMP antagonists. In contrast, in Drosophila embryos, Toll-like receptor (Tlr)-mediated NFκB activation initiates dorsal-ventral patterning, wherein Wnt-mediated negative feedback regulation of Tlr/NFκB generates a BMP antagonist-secreting signalling centre to control the dorsal-ventral pattern. Although both Wnt and BMP antagonist are conserved among species, the involvement of Tlr/NFκB and feedback regulation in vertebrate organizer formation remains unclear. By imaging and genetic modification, we reveal that a negative feedback loop between canonical and non-canonical Wnts and Tlr4/NFκB determines the size of zebrafish organizer, and that Tlr/NFκB and Wnts switch initial cue and feedback mediator roles between Drosophila and zebrafish. Here, we show that canonical Wnt signalling stimulates the expression of the non-canonical Wnt5b ligand, activating the Tlr4 receptor to stimulate NFκB-mediated transcription of the Wnt antagonist frzb, restricting Wnt-dependent dorsal organizer formation.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93372
citation_title	Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB
citation_author	Zou, Juqi
	Anai, Satoshi
	Ota, Satoshi
	Ishitani, Shizuka
	Oginuma, Masayuki
	Ishitani, Tohru
citation_publisher	Nature Research
citation_language	英語
citation_date	2023-11-08
citation_journal_title	Nature Communications
citation_volume	14
citation_firstpage	7194
citation_doi	https://doi.org/10.1038/s41467-023-42963-3
citation_public_url	https://hdl.handle.net/11094/93372

Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB

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