学術雑誌論文

学術雑誌論文

text

Nature Methods. 2022, 19(10), p. 1286-1294

A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain

Nature Research

英語

text

2022-10-01

https://doi.org/10.1038/s41592-022-01597-x

Ino D., Tanaka Y., Hibino H., et al. A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain. Nature Methods 19, 1286 (2022); https://doi.org/10.1038/s41592-022-01597-x.

Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIAOT, a G-protein-coupled receptor-based green fluorescent OT sensor that has a large dynamic range, suitable affinity, ligand specificity for OT orthologs, minimal effects on downstream signaling and long-term fluorescence stability. By combining viral gene delivery and fiber photometry-mediated fluorescence measurements, we demonstrate the utility of MTRIAOT for real-time detection of brain OT dynamics in living mice. MTRIAOT-mediated measurements indicate variability of OT dynamics depending on the behavioral context and physical condition of an animal. MTRIAOT will likely enable the analysis of OT dynamics in a variety of physiological and pathological processes.

This article is licensed under a Creative Commons Attribution 4.0 International License.

93409

A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain

Nature Research

英語

2022-10-01

Nature Methods

19

10

1286

1294

https://doi.org/10.1038/s41592-022-01597-x

https://hdl.handle.net/11094/93409