Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae

Nin, Fumiaki; Choi, Samuel; Ota, Takeru; Qi, Zhang; Hibino, Hiroshi

doi:https://doi.org/10.1007/s10043-021-00654-8

Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae

Nin, Fumiaki; Choi, Samuel; Ota, Takeru et al.

Optical Review, 2021, 28(2), 239-254

アクセス数:650件（2025-08-15 06:44 集計）

固定URL: https://hdl.handle.net/11094/93413

閲覧可能ファイル

ファイル	フォーマット	利用条件	サイズ	閲覧回数	利用開始日	説明	information
OptRev_28_2_239	pdf	なし	1.81 MB	59	2024.01.15

Creative Commons : 表示

論文情報

ファイル出力 EndNote Basic出力 Mendeley出力

タイトル	Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae
著者	NRID 1000080644905 1000080644905 著者名 Nin, Fumiaki
	NRID 1000060568418 1000060568418 著者名 Choi, Samuel
	NRID 1000030790571 1000030790571 ORCID 0000-0003-0011-8925 0000-0003-0011-8925 researchmap permalink otatakeru otatakeru Scopus Author ID 26642091900 26642091900 著者名 Ota, Takeru
	著者名 Qi, Zhang
	NRID 1000070314317 1000070314317 researchmap permalink hiroshi_hibino hiroshi_hibino Scopus Author ID 7007020846 7007020846 著者名 Hibino, Hiroshi
キーワード等	Cochlear partition
	In vivo vibrometry
	Optical coherence tomography
	Organ of Corti
	Outer hair cells
	Supercontinuum
内容	The version of record of this article, first published in Optical Review, is available online at Publisher’s website: https://doi.org/10.1007/s10043-021-00654-8.
抄録	Sound evokes sub-nanoscale vibration within the sensory epithelium. The epithelium contains not only immotile cells but also contractile outer hair cells (OHCs) that actively shrink and elongate synchronously with the sound. However, the in vivo motion of OHCs has remained undetermined. The aim of this work is to perform high-resolution and -accuracy vibrometry in live guinea pigs with an SC-introduced spectral-domain optical coherence tomography system (SD-OCT). In this study, to reveal the effective contribution of SC source in the recording of the low reflective materials with the short total acquisition time, we compare the performances of the SC-introduced SD-OCT (SCSD-OCT) to that of the conventional SD-OCT. As inanimate comparison objects, we record a mirror, a piezo actuator, and glass windows. For the measurements in biological materials, we use in/ex vivo guinea pig cochleae. Our study achieved the optimization of a SD-OCT system for high-resolution in vivo vibrometry in the cochlear sensory epithelium, termed the organ of Corti, in mammalian cochlea. By introducing a supercontinuum (SC) light source and reducing the total acquisition time, we improve the axial resolution and overcome the difficulty in recording the low reflective material in the presence of biological noise. The high power of the SC source enables the system to achieve a spatial resolution of 1.72 ± 0.00 μm on a mirror and reducing the total acquisition time contributes to the high spatial accuracy of sub-nanoscale vibrometry. Our findings reveal the vibrations at the apical/basal region of OHCs and the extracellular matrix, basilar membrane.
出版者	Springer Science and Business Media LLC
収録物名	Optical Review
巻(号)	28(2)
ページ	239-254
刊行年月	2021-04-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93413
PISSN	13406000
eISSN	13499432
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1007/s10043-021-00654-8
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Optical Review. 2021, 28(2), p. 239-254
DC.title	Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae
DC.creator	Nin, Fumiaki
	Choi, Samuel
	Ota, Takeru
	Qi, Zhang
	Hibino, Hiroshi
DC.publisher	Springer Science and Business Media LLC
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2021-04-01
DC.identifier	https://doi.org/10.1007/s10043-021-00654-8
DC.subject	Cochlear partition
	In vivo vibrometry
	Optical coherence tomography
	Organ of Corti
	Outer hair cells
	Supercontinuum
DC.description	The version of record of this article, first published in Optical Review, is available online at Publisher’s website: https://doi.org/10.1007/s10043-021-00654-8.
DCTERMS.abstract	Sound evokes sub-nanoscale vibration within the sensory epithelium. The epithelium contains not only immotile cells but also contractile outer hair cells (OHCs) that actively shrink and elongate synchronously with the sound. However, the in vivo motion of OHCs has remained undetermined. The aim of this work is to perform high-resolution and -accuracy vibrometry in live guinea pigs with an SC-introduced spectral-domain optical coherence tomography system (SD-OCT). In this study, to reveal the effective contribution of SC source in the recording of the low reflective materials with the short total acquisition time, we compare the performances of the SC-introduced SD-OCT (SCSD-OCT) to that of the conventional SD-OCT. As inanimate comparison objects, we record a mirror, a piezo actuator, and glass windows. For the measurements in biological materials, we use in/ex vivo guinea pig cochleae. Our study achieved the optimization of a SD-OCT system for high-resolution in vivo vibrometry in the cochlear sensory epithelium, termed the organ of Corti, in mammalian cochlea. By introducing a supercontinuum (SC) light source and reducing the total acquisition time, we improve the axial resolution and overcome the difficulty in recording the low reflective material in the presence of biological noise. The high power of the SC source enables the system to achieve a spatial resolution of 1.72 ± 0.00 μm on a mirror and reducing the total acquisition time contributes to the high spatial accuracy of sub-nanoscale vibrometry. Our findings reveal the vibrations at the apical/basal region of OHCs and the extracellular matrix, basilar membrane.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93413
citation_title	Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae
citation_author	Nin, Fumiaki
	Choi, Samuel
	Ota, Takeru
	Qi, Zhang
	Hibino, Hiroshi
citation_publisher	Springer Science and Business Media LLC
citation_language	英語
citation_date	2021-04-01
citation_journal_title	Optical Review
citation_volume	28
citation_issue	2
citation_firstpage	239
citation_lastpage	254
citation_doi	https://doi.org/10.1007/s10043-021-00654-8
citation_public_url	https://hdl.handle.net/11094/93413

Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae

閲覧可能ファイル

論文情報

論文詳細を表示