Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons

Atsumi, Yuri; Iwata, Ryohei; Kimura, Hiroshi; Vanderhaeghen, Pierre; Yamamoto, Nobuhiko; Sugo, Noriyuki

doi:https://doi.org/10.1016/j.celrep.2023.113576

Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons

Atsumi, Yuri; Iwata, Ryohei; Kimura, Hiroshi et al.

Cell Reports, 2023, 3, 113576

アクセス数:841件（2026-04-05 00:42 集計）

固定URL: https://hdl.handle.net/11094/93526

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タイトル	Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons
著者	著者名 Atsumi, Yuri
	著者名 Iwata, Ryohei
	NRID 1000030241392 1000030241392 著者名 Kimura, Hiroshi
	著者名 Vanderhaeghen, Pierre
	NRID 1000000191429 1000000191429 researchmap permalink nobuhikoyamamoto nobuhikoyamamoto 著者名 Yamamoto, Nobuhiko
	NRID 1000020372625 1000020372625 researchmap permalink norisugo norisugo Scopus Author ID 6602838748 6602838748 著者名 Sugo, Noriyuki
内容	Atsumi Yuri, Iwata Ryohei, Kimura Hiroshi, et al. Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons. Cell Reports 3, 113576 (2023); https://doi.org/10.1016/j.celrep.2023.113576.
抄録	Neuronal activity-dependent transcription plays a key role in plasticity and pathology in the brain. An intriguing question is how neuronal activity controls gene expression via interactions of transcription factors with DNA and chromatin modifiers in the nucleus. By utilizing single-molecule imaging in human embryonic stem cell (ESC)-derived cortical neurons, we demonstrate that neuronal activity increases repetitive emergence of cAMP response element-binding protein (CREB) at histone acetylation sites in the nucleus, where RNA polymerase II (RNAPII) accumulation and FOS expression occur rapidly. Neuronal activity also enhances co-localization of CREB and CREB-binding protein (CBP). Increased binding of a constitutively active CREB to CBP efficiently induces CREB repetitive emergence. On the other hand, the formation of histone acetylation sites is dependent on CBP histone modification via acetyltransferase (HAT) activity but is not affected by neuronal activity. Taken together, our results suggest that neuronal activity promotes repetitive CREB-CRE and CREB-CBP interactions at predetermined histone acetylation sites, leading to rapid gene expression.
出版者	Elsevier BV
収録物名	Cell Reports
巻(号)	3
ページ	113576
刊行年月	2023-12-18
言語	英語
ハンドルURL	https://hdl.handle.net/11094/93526
PISSN	22111247
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.celrep.2023.113576
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Cell Reports. 2023, 3, p. 113576
DC.title	Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons
DC.creator	Atsumi, Yuri
	Iwata, Ryohei
	Kimura, Hiroshi
	Vanderhaeghen, Pierre
	Yamamoto, Nobuhiko
	Sugo, Noriyuki
DC.publisher	Elsevier BV
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-12-18
DC.identifier	https://doi.org/10.1016/j.celrep.2023.113576
DC.description	Atsumi Yuri, Iwata Ryohei, Kimura Hiroshi, et al. Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons. Cell Reports 3, 113576 (2023); https://doi.org/10.1016/j.celrep.2023.113576.
DCTERMS.abstract	Neuronal activity-dependent transcription plays a key role in plasticity and pathology in the brain. An intriguing question is how neuronal activity controls gene expression via interactions of transcription factors with DNA and chromatin modifiers in the nucleus. By utilizing single-molecule imaging in human embryonic stem cell (ESC)-derived cortical neurons, we demonstrate that neuronal activity increases repetitive emergence of cAMP response element-binding protein (CREB) at histone acetylation sites in the nucleus, where RNA polymerase II (RNAPII) accumulation and FOS expression occur rapidly. Neuronal activity also enhances co-localization of CREB and CREB-binding protein (CBP). Increased binding of a constitutively active CREB to CBP efficiently induces CREB repetitive emergence. On the other hand, the formation of histone acetylation sites is dependent on CBP histone modification via acetyltransferase (HAT) activity but is not affected by neuronal activity. Taken together, our results suggest that neuronal activity promotes repetitive CREB-CRE and CREB-CBP interactions at predetermined histone acetylation sites, leading to rapid gene expression.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	93526
citation_title	Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons
citation_author	Atsumi, Yuri
	Iwata, Ryohei
	Kimura, Hiroshi
	Vanderhaeghen, Pierre
	Yamamoto, Nobuhiko
	Sugo, Noriyuki
citation_publisher	Elsevier BV
citation_language	英語
citation_date	2023-12-18
citation_journal_title	Cell Reports
citation_volume	3
citation_firstpage	113576
citation_doi	https://doi.org/10.1016/j.celrep.2023.113576
citation_public_url	https://hdl.handle.net/11094/93526

Repetitive CREB-DNA interactions at gene loci predetermined by CBP induce activity-dependent gene expression in human cortical neurons

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