学術雑誌論文

学術雑誌論文

text

Cell Reports. 2024, 43, p. 113599

Pib2 is a cysteine sensor involved in TORC1 activation in Saccharomyces cerevisiae

Elsevier B.V.

英語

text

2024-01-23

https://doi.org/10.1016/j.celrep.2023.113599

Zeng Q., Araki Y., Noda T.. Pib2 is a cysteine sensor involved in TORC1 activation in Saccharomyces cerevisiae. Cell Reports 43, 113599 (2024); https://doi.org/10.1016/j.celrep.2023.113599.

Target of rapamycin complex 1 (TORC1) is a master regulator that monitors the availability of various amino acids to promote cell growth in Saccharomyces cerevisiae. It is activated via two distinct upstream pathways: the Gtr pathway, which corresponds to mammalian Rag, and the Pib2 pathway. This study shows that Ser3 was phosphorylated exclusively in a Pib2-dependent manner. Using Ser3 as an indicator of TORC1 activity, together with the established TORC1 substrate Sch9, we investigated which pathways were employed by individual amino acids. Different amino acids exhibited different dependencies on the Gtr and Pib2 pathways. Cysteine was most dependent on the Pib2 pathway and increased the interaction between TORC1 and Pib2 in vivo and in vitro. Moreover, cysteine directly bound to Pib2 via W632 and F635, two critical residues in the T(ail) motif that are necessary to activate TORC1. These results indicate that Pib2 functions as a sensor for cysteine in TORC1 regulation.

This article is licensed under a Creative Commons Attribution 4.0 International License.

93531

Pib2 is a cysteine sensor involved in TORC1 activation in Saccharomyces cerevisiae

Elsevier B.V.

英語

2024-01-23

Cell Reports

43

113599

https://doi.org/10.1016/j.celrep.2023.113599

https://hdl.handle.net/11094/93531