学術雑誌論文

学術雑誌論文

text

Genes to Cells. 2023, 28(11), p. 776-788

Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia

John Wiley and Sons Inc

英語

text

2023-11-01

https://doi.org/10.1111/gtc.13065

This is the peer reviewed version of the following article: Nakatani A., Okumura R., Ishibashi A., et al. Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia. Genes to Cells 28, 776 (2023), which has been published in final form at https://doi.org/10.1111/gtc.13065. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.

In the intestine, interleukin (IL)-23 and IL-22 from immune cells in the lamina propria contribute to maintenance of the gut epithelial barrier through the induction of antimicrobial production and the promotion of epithelial cell proliferation. Several previous studies suggested that some of the functions of the IL-23/IL-22 axis on intestinal epithelial cells are shared between the small and large intestines. However, the similarities and differences of the IL-23/IL-22 axis on epithelial cells between these two anatomical sites remain unclear. Here, we comprehensively analyzed the gene expression of intestinal epithelial cells in the ileum and colon of germ-free, Il23−/−, and Il22−/− mice by RNA-sequencing. We found that while the IL-23/IL-22 axis is largely dependent on gut microbiota in the small intestine, it is much less dependent on it in the large intestine. In addition, the negative regulation of lipid metabolism in the epithelial cells by IL-23 and IL-22 in the small intestine was revealed, whereas the positive regulation of epithelial cell proliferation by IL-23 and IL-22 in the large intestine was highlighted. These findings shed light on the intestinal site-specific role of the IL-23/IL-22 axis in maintaining the physiological functions of intestinal epithelial cells.

© 2023 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.

93980

Differential dependence on microbiota of IL-23/IL-22-dependent gene expression between the small- and large-intestinal epithelia

John Wiley and Sons Inc

英語

2023-11-01

Genes to Cells

28

11

776

788

https://doi.org/10.1111/gtc.13065

https://hdl.handle.net/11094/93980