Antibodies reactive with the N-terminal domain of Plasmodium falciparum serine repeat antigen inhibit cell proliferation by agglutinating merozoites and schizonts

Pang, Xin Li; Mitamura, Toshihide; Horii, Toshihiro

Infection and Immunity, 1999, 67(4), 1821-1827

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固定URL: https://hdl.handle.net/11094/94548

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タイトル
Antibodies reactive with the N-terminal domain of Plasmodium falciparum serine repeat antigen inhibit cell proliferation by agglutinating merozoites and schizonts
著者
著者名
Pang, Xin Li
NRID
1000080268846 1000080268846
著者名
Mitamura, Toshihide
NRID
1000080142305 1000080142305
ORCID
0000-0002-3000-2045 0000-0002-3000-2045
researchmap permalink
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Scopus Author ID
7102402467 7102402467
著者名
Horii, Toshihiro
内容
Copyright © American Society for Microbiology, Infection and Immunity, 67(4), 1999, 1821-1827, https://doi.org/10.1128/.67.4.1821-1827.1999
抄録
The serine repeat antigen (sera) is a vaccine candidate antigen of Plasmodium falciparum. Immunization of mice with Escherichia coli-produced recombinant protein of the Sera N-terminal domain (SE47') induced an antiserum that was inhibitory to parasite growth in vitro. Affinity-purified mouse antibodies specific to the recombinant protein inhibited parasite growth between the schizont and ring stages but not between the ring and schizont stages. When Percoll-purified schizonts were cultured with the affinity-purified SE47'-specific antibodies, schizonts and merozoites were agglutinated. Indirect-immunofluorescence assays with unfixed parasite cells showed that SE47'-specific immunoglobulin G (IgG) bound to Sera molecules on rupturing schizonts and merozoites but the IgG did not react with the schizont-infected erythrocytes (RBC). Furthermore, double-fluorescence staining against SE47'-specific IgG and anti-human RBC membrane IgG showed that the Rbc membrane disappeared from SE47'-specific-IgG-bound schizonts after cultivation. These observations suggest that the SE47'-specific antibodies inhibit parasite growth by cross-linking Sera molecules that are associated with merozoites in rupturing schizonts with partly broken RBC and parasitophorous vacuole membranes, blocking merozoite release.
出版者
American Society for Microbiology
収録物名
Infection and Immunity
巻(号)
67(4)
ページ
1821-1827
刊行年月
1999-04-01
言語
英語
ハンドルURL
https://hdl.handle.net/11094/94548
PISSN
00199567
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1128/.67.4.1821-1827.1999
アクセス権
open access
権利情報
Copyright © 1999, American Society for Microbiology. All Rights Reserved.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article