Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts

Zhang, Jingbo; Li, Junjun; Qu, Xiang; Liu, Yuting; Harada, Akima; Hua, Ying; Yoshida, Noriko; Ishida, Masako; Tabata, Akiko; Sun, Lifu; Liu, Li; Miyagawa, Shigeru

doi:https://doi.org/10.1186/s13287-023-03560-9

Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts

Zhang, Jingbo; Li, Junjun; Qu, Xiang et al.

Stem Cell Research and Therapy, 2023, 14, 380

アクセス数:55件（2025-05-10 03:12 集計）

固定URL: https://hdl.handle.net/11094/94588

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タイトル	Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts
著者	著者名 Zhang, Jingbo
	NRID 1000010723786 1000010723786 ORCID 0000-0002-7473-4918 0000-0002-7473-4918 researchmap permalink liJJ liJJ Scopus Author ID 52464043600 52464043600 著者名 Li, Junjun
	著者名 Qu, Xiang
	著者名 Liu, Yuting
	NRID 1000070535426 1000070535426 著者名 Harada, Akima
	著者名 Hua, Ying
	著者名 Yoshida, Noriko
	著者名 Ishida, Masako
	著者名 Tabata, Akiko
	著者名 Sun, Lifu
	NRID 1000050380093 1000050380093 researchmap permalink 7000008694 7000008694 Scopus Author ID 55628530013 55628530013 著者名 Liu, Li
	NRID 1000070544237 1000070544237 ORCID 0000-0003-0015-6569 0000-0003-0015-6569 researchmap permalink s-miyagawa3154 s-miyagawa3154 Scopus Author ID 55560624700 55560624700 著者名 Miyagawa, Shigeru
キーワード等	Adipose-derived mesenchymal stem cell
	Angiogenesis
	Myocardial infarction
	Regenerative medicine
	Tissue engineering
内容	Zhang J., Li J., Qu X., et al. Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts. Stem Cell Research and Therapy 14, 380 (2023); https://doi.org/10.1186/s13287-023-03560-9.
抄録	Background: Heart failure (HF) is a major cause of death worldwide. The most effective treatment for HF is heart transplantation, but its use is limited by the scarcity of donor hearts. Recently, stem cell-based therapy has emerged as a promising approach for treating myocardial infarction. Our research group has been investigating the use of human induced pluripotent stem cell-derived cardiomyocyte patches as a potential therapeutic candidate. We have successfully conducted eight cases of clinical trials and demonstrated the safety and effectiveness of this approach. However, further advancements are necessary to overcome immune rejection and enhance therapeutic efficacy. In this study, we propose a novel and efficient technique for constructing mesenchymal stem cell (MSC) tissue sheets, which can be transplanted effectively for treating myocardial infarction repair. Methods: We applied a one-step method to construct the human adipose-derived mesenchymal stem cell (hADSC) tissue sheet on a poly(lactic-co-glycolic acid) fiber scaffold. Histology, immunofluorescence, and paracrine profile assessment were used to determine the organization and function of the hADSC tissue sheet. Echocardiography and pathological analyses of heart sections were performed to evaluate cardiac function, fibrosis area, angiogenesis, and left ventricular remodeling. Results: In vitro, the hADSC tissue sheet showed great organization, abundant ECM expression, and increased paracrine secretion than single cells. In vivo, the hADSC tissue sheet group demonstrated improved cardiac functional recovery, less ventricular remodeling, decreased fibrosis, and enhanced angiogenesis than the MI group. Conclusions: We developed thick and functional hADSC tissue sheets via the one-step strategy. The hADSC tissue sheet showed excellent performance in treating myocardial infarction in the rat model.
出版者	BioMed Central Ltd
収録物名	Stem Cell Research and Therapy
巻(号)	14
ページ	380
刊行年月	2023-12-20
言語	英語
ハンドルURL	https://hdl.handle.net/11094/94588
eISSN	17576512
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1186/s13287-023-03560-9
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

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資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Stem Cell Research and Therapy. 2023, 14, p. 380
DC.title	Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts
DC.creator	Zhang, Jingbo
	Li, Junjun
	Qu, Xiang
	Liu, Yuting
	Harada, Akima
	Hua, Ying
	Yoshida, Noriko
	Ishida, Masako
	Tabata, Akiko
	Sun, Lifu
	Liu, Li
	Miyagawa, Shigeru
DC.publisher	BioMed Central Ltd
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-12-20
DC.identifier	https://doi.org/10.1186/s13287-023-03560-9
DC.subject	Adipose-derived mesenchymal stem cell
	Angiogenesis
	Myocardial infarction
	Regenerative medicine
	Tissue engineering
DC.description	Zhang J., Li J., Qu X., et al. Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts. Stem Cell Research and Therapy 14, 380 (2023); https://doi.org/10.1186/s13287-023-03560-9.
DCTERMS.abstract	Background: Heart failure (HF) is a major cause of death worldwide. The most effective treatment for HF is heart transplantation, but its use is limited by the scarcity of donor hearts. Recently, stem cell-based therapy has emerged as a promising approach for treating myocardial infarction. Our research group has been investigating the use of human induced pluripotent stem cell-derived cardiomyocyte patches as a potential therapeutic candidate. We have successfully conducted eight cases of clinical trials and demonstrated the safety and effectiveness of this approach. However, further advancements are necessary to overcome immune rejection and enhance therapeutic efficacy. In this study, we propose a novel and efficient technique for constructing mesenchymal stem cell (MSC) tissue sheets, which can be transplanted effectively for treating myocardial infarction repair. Methods: We applied a one-step method to construct the human adipose-derived mesenchymal stem cell (hADSC) tissue sheet on a poly(lactic-co-glycolic acid) fiber scaffold. Histology, immunofluorescence, and paracrine profile assessment were used to determine the organization and function of the hADSC tissue sheet. Echocardiography and pathological analyses of heart sections were performed to evaluate cardiac function, fibrosis area, angiogenesis, and left ventricular remodeling. Results: In vitro, the hADSC tissue sheet showed great organization, abundant ECM expression, and increased paracrine secretion than single cells. In vivo, the hADSC tissue sheet group demonstrated improved cardiac functional recovery, less ventricular remodeling, decreased fibrosis, and enhanced angiogenesis than the MI group. Conclusions: We developed thick and functional hADSC tissue sheets via the one-step strategy. The hADSC tissue sheet showed excellent performance in treating myocardial infarction in the rat model.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	94588
citation_title	Development of a thick and functional human adipose-derived stem cell tissue sheet for myocardial infarction repair in rat hearts
citation_author	Zhang, Jingbo
	Li, Junjun
	Qu, Xiang
	Liu, Yuting
	Harada, Akima
	Hua, Ying
	Yoshida, Noriko
	Ishida, Masako
	Tabata, Akiko
	Sun, Lifu
	Liu, Li
	Miyagawa, Shigeru
citation_publisher	BioMed Central Ltd
citation_language	英語
citation_date	2023-12-20
citation_journal_title	Stem Cell Research and Therapy
citation_volume	14
citation_firstpage	380
citation_doi	https://doi.org/10.1186/s13287-023-03560-9
citation_public_url	https://hdl.handle.net/11094/94588