Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

Li, Junjun; Hua, Ying; Liu, Yuting; Qu, Xiang; Zhang, Jingbo; Ishida, Masako; Yoshida, Noriko; Tabata, Akiko; Miyoshi, Hayato; Shiba, Mikio; Higo, Shuichiro; Sougawa, Nagako; Takeda, Maki; Kawamura, Takuji; Matsuura, Ryohei; Okuzaki, Daisuke; Toyofuku, Toshihiko; Sawa, Yoshiki; Liu, Li; Miyagawa, Shigeru

doi:https://doi.org/10.1016/j.isci.2024.108992

Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

Li, Junjun; Hua, Ying; Liu, Yuting et al.

iScience, 2024, 27(2), 108992

アクセス数:106件（2025-04-27 00:35 集計）

固定URL: https://hdl.handle.net/11094/94598

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タイトル	Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment
著者	NRID 1000010723786 1000010723786 ORCID 0000-0002-7473-4918 0000-0002-7473-4918 researchmap permalink liJJ liJJ Scopus Author ID 52464043600 52464043600 著者名 Li, Junjun
	著者名 Hua, Ying
	著者名 Liu, Yuting
	著者名 Qu, Xiang
	著者名 Zhang, Jingbo
	著者名 Ishida, Masako
	著者名 Yoshida, Noriko
	著者名 Tabata, Akiko
	著者名 Miyoshi, Hayato
	著者名 Shiba, Mikio
	NRID 1000000604034 1000000604034 researchmap permalink s.higo s.higo Scopus Author ID 23018633200 23018633200 著者名 Higo, Shuichiro
	NRID 1000030432579 1000030432579 researchmap permalink SougawaNagako SougawaNagako 著者名 Sougawa, Nagako
	著者名 Takeda, Maki
	NRID 1000060839398 1000060839398 researchmap permalink takuji-kawamura takuji-kawamura Scopus Author ID 55331768500 55331768500 著者名 Kawamura, Takuji
	NRID 1000010768922 1000010768922 researchmap permalink ryoheimatsuura ryoheimatsuura Scopus Author ID 57195988109 57195988109 著者名 Matsuura, Ryohei
	NRID 1000000346131 1000000346131 ORCID 0000-0002-4552-783X 0000-0002-4552-783X researchmap permalink read0092307 read0092307 Scopus Author ID 6506703054 6506703054 著者名 Okuzaki, Daisuke
	NRID 1000060322179 1000060322179 著者名 Toyofuku, Toshihiko
	NRID 1000000243220 1000000243220 researchmap permalink y-sawa0703 y-sawa0703 Scopus Author ID 55428162700 55428162700 著者名 Sawa, Yoshiki
	NRID 1000050380093 1000050380093 researchmap permalink 7000008694 7000008694 Scopus Author ID 55628530013 55628530013 著者名 Liu, Li
	NRID 1000070544237 1000070544237 ORCID 0000-0003-0015-6569 0000-0003-0015-6569 researchmap permalink s-miyagawa3154 s-miyagawa3154 Scopus Author ID 55560624700 55560624700 著者名 Miyagawa, Shigeru
キーワード等	Bioengineering
	Biotechnology
	Cardiovascular medicine
	Stem cells research
内容	Li J., Hua Y., Liu Y., et al. Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment. iScience 27, 108992 (2024); https://doi.org/10.1016/j.isci.2024.108992.
抄録	Human iPSC-derived cardiomyocytes (hiPSC-CMs) exhibit functional immaturity, potentially impacting their suitability for assessing drug proarrhythmic potential. We previously devised a traveling wave (TW) system to promote maturation in 3D cardiac tissue. To align with current drug assessment paradigms (CiPA and JiCSA), necessitating a 2D monolayer cardiac tissue, we integrated the TW system with a multi-electrode array. This gave rise to a hiPSC-derived closed-loop cardiac tissue (iCT), enabling spontaneous TW initiation and swift pacing of cardiomyocytes from various cell lines. The TW-paced cardiomyocytes demonstrated heightened sarcomeric and functional maturation, exhibiting enhanced response to isoproterenol. Moreover, these cells showcased diminished sensitivity to verapamil and maintained low arrhythmia rates with ranolazine—two drugs associated with a low risk of torsades de pointes (TdP). Notably, the TW group displayed increased arrhythmia rates with high and intermediate risk TdP drugs (quinidine and pimozide), underscoring the potential utility of this system in drug assessment applications.
出版者	Elsevier Inc.
収録物名	iScience
巻(号)	27(2)
ページ	108992
刊行年月	2024-02-16
言語	英語
ハンドルURL	https://hdl.handle.net/11094/94598
eISSN	25890042
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.isci.2024.108992
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ	Version of Record
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	iScience. 2024, 27(2), p. 108992
DC.title	Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment
DC.creator	Li, Junjun
	Hua, Ying
	Liu, Yuting
	Qu, Xiang
	Zhang, Jingbo
	Ishida, Masako
	Yoshida, Noriko
	Tabata, Akiko
	Miyoshi, Hayato
	Shiba, Mikio
	Higo, Shuichiro
	Sougawa, Nagako
	Takeda, Maki
	Kawamura, Takuji
	Matsuura, Ryohei
	Okuzaki, Daisuke
	Toyofuku, Toshihiko
	Sawa, Yoshiki
	Liu, Li
	Miyagawa, Shigeru
DC.publisher	Elsevier Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-02-16
DC.identifier	https://doi.org/10.1016/j.isci.2024.108992
DC.subject	Bioengineering
	Biotechnology
	Cardiovascular medicine
	Stem cells research
DC.description	Li J., Hua Y., Liu Y., et al. Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment. iScience 27, 108992 (2024); https://doi.org/10.1016/j.isci.2024.108992.
DCTERMS.abstract	Human iPSC-derived cardiomyocytes (hiPSC-CMs) exhibit functional immaturity, potentially impacting their suitability for assessing drug proarrhythmic potential. We previously devised a traveling wave (TW) system to promote maturation in 3D cardiac tissue. To align with current drug assessment paradigms (CiPA and JiCSA), necessitating a 2D monolayer cardiac tissue, we integrated the TW system with a multi-electrode array. This gave rise to a hiPSC-derived closed-loop cardiac tissue (iCT), enabling spontaneous TW initiation and swift pacing of cardiomyocytes from various cell lines. The TW-paced cardiomyocytes demonstrated heightened sarcomeric and functional maturation, exhibiting enhanced response to isoproterenol. Moreover, these cells showcased diminished sensitivity to verapamil and maintained low arrhythmia rates with ranolazine—two drugs associated with a low risk of torsades de pointes (TdP). Notably, the TW group displayed increased arrhythmia rates with high and intermediate risk TdP drugs (quinidine and pimozide), underscoring the potential utility of this system in drug assessment applications.
DC.rights	This article is licensed under a Creative Commons Attribution 4.0 International License.
DC.format	94598
citation_title	Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment
citation_author	Li, Junjun
	Hua, Ying
	Liu, Yuting
	Qu, Xiang
	Zhang, Jingbo
	Ishida, Masako
	Yoshida, Noriko
	Tabata, Akiko
	Miyoshi, Hayato
	Shiba, Mikio
	Higo, Shuichiro
	Sougawa, Nagako
	Takeda, Maki
	Kawamura, Takuji
	Matsuura, Ryohei
	Okuzaki, Daisuke
	Toyofuku, Toshihiko
	Sawa, Yoshiki
	Liu, Li
	Miyagawa, Shigeru
citation_publisher	Elsevier Inc.
citation_language	英語
citation_date	2024-02-16
citation_journal_title	iScience
citation_volume	27
citation_issue	2
citation_firstpage	108992
citation_doi	https://doi.org/10.1016/j.isci.2024.108992
citation_public_url	https://hdl.handle.net/11094/94598

Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

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