Integrin α5 regulates motility of human monocyte-derived Langerhans cells during immune response

Guo, Zhihan; Murakami, Masato; Saito, Kaori et al.

Experimental Dermatology, 2024, 33(3), e15021

アクセス数:679件（2025-08-21 03:03 集計）

固定URL: https://hdl.handle.net/11094/95266

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タイトル
Integrin α5 regulates motility of human monocyte-derived Langerhans cells during immune response
著者
著者名
Guo, Zhihan
著者名
Murakami, Masato
NRID
1000080836924 1000080836924
researchmap permalink
kaosai kaosai
著者名
Saito, Kaori
NRID
1000070749994 1000070749994
ORCID
0000-0002-2618-5539 0000-0002-2618-5539
researchmap permalink
hirokokato hirokokato
Scopus Author ID
55314208200 55314208200
著者名
Kato, Hiroko
著者名
Toriyama, Manami
NRID
1000090260041 1000090260041
著者名
Tominaga, Makoto
著者名
Ishii, Ken J.
NRID
1000070600097 1000070600097
researchmap permalink
fufufufu fufufufu
著者名
Fujita, Fumitaka
キーワード等
cell adhesion
cell motility
inflammation
integrins
Langerhans cells
live cell imaging
内容
Guo Z., Murakami M., Saito K., et al. Integrin α5 regulates motility of human monocyte-derived Langerhans cells during immune response. Experimental Dermatology 33, e15021 (2024); https://doi.org/10.1111/exd.15021.
抄録
Langerhans cells (LCs) are mainly present in the epidermis and mucosa, and have important roles during skin infection. Migration of LCs to lymph nodes is essential for antigen presentation. However, due to the difficulties in isolating and culturing human LCs, it is not fully understood how LCs move and interact with the extracellular matrix (ECM) through their adhesion molecules such as integrin, during the immune responses. In this study, we aimed to investigate LC motility, cell shape and the role of integrin under inflammatory conditions using monocyte-derived Langerhans cells (moLCs) as a model. As a result, lipopolysaccharide (LPS) stimulation increased adhesion on fibronectin coated substrate and integrin α5 expression in moLCs. Time-lapse imaging of moLCs revealed that stimulation with LPS elongated cell shape, whilst decreasing their motility. Additionally, this decrease in motility was not observed when pre-treated with a neutralising antibody targeting integrin α5. Together, our data suggested that activation of LCs decreases their motility by promoting integrin α5 expression to enhance their affinity to the fibronectin, which may contribute to their migration during inflammation.
出版者
John Wiley and Sons Inc
収録物名
Experimental Dermatology
巻(号)
33(3)
ページ
e15021
刊行年月
2024-03-01
言語
英語
ハンドルURL
https://hdl.handle.net/11094/95266
PISSN
09066705
eISSN
16000625
関連情報 (isIdenticalTo)
DOI (出版社版)
https://doi.org/10.1111/exd.15021
アクセス権
open access
権利情報
This article is licensed under a Creative Commons Attribution 4.0 International License.
出版タイプ
Version of Record
カテゴリ
学術雑誌論文 Journal Article