Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease

Kinoshita, Riku; Inoue, Naoya; Iwatani, Yoshinori; Noguchi, Yusuke; Hidaka, Yoh; Watanabe, Mikio

doi:https://doi.org/10.1016/j.clim.2024.110196

Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease

Kinoshita, Riku; Inoue, Naoya; Iwatani, Yoshinori et al.

Clinical Immunology, 2024, 263, 110196

アクセス数:642件（2025-07-07 12:41 集計）

固定URL: https://hdl.handle.net/11094/95449

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タイトル	Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease
著者	著者名 Kinoshita, Riku
	著者名 Inoue, Naoya
	NRID 1000060168581 1000060168581 researchmap permalink read0013695 read0013695 著者名 Iwatani, Yoshinori
	著者名 Noguchi, Yusuke
	NRID 1000030243231 1000030243231 researchmap permalink Y99 Y99 Scopus Author ID 7102099529 7102099529 著者名 Hidaka, Yoh
	NRID 1000050294088 1000050294088 ORCID 0000-0001-5971-9296 0000-0001-5971-9296 researchmap permalink watanabemikio watanabemikio Scopus Author ID 55793188612 55793188612 著者名 Watanabe, Mikio
キーワード等	Autoimmune thyroid disease
	IL-10
	Intractability
	Methylation
	Polymorphism
	Pyrosequencing
	Severity
内容	Kinoshita R., Inoue N., Iwatani Y., et al. Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease. Clinical Immunology 263, 110196 (2024); https://doi.org/10.1016/j.clim.2024.110196.
抄録	The prognosis of autoimmune thyroid diseases (AITDs), including Hashimoto's disease (HD) and Graves' disease (GD), is difficult to predict. DNA methylation regulates gene expression of immune mediating factors. Interleukin (IL)-10 is a Th2 cytokine that downregulates inflammatory cytokines produced by Th1 cells. To clarify the role of methylation of the IL10 gene in the prognosis of AITD, we evaluated the methylation levels of two CpG sites in the IL10 promoter using pyrosequencing. The methylation levels of the −185 CpG site of the IL10 gene were related to age and GD intractability in GD patients. Furthermore, the C carrier of the IL10–592 A/C polymorphism was related to low methylation levels of the −185 CpG site. The methylation levels of the IL10–185 CpG site of the IL10 gene were related to the intractability of GD and were lower in individuals with the C allele of the IL10–592 A/C polymorphism.
出版者	Academic Press Inc.
収録物名	Clinical Immunology
巻(号)	263
ページ	110196
刊行年月	2024-06-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/95449
PISSN	15216616
eISSN	15217035
関連情報 (isVersionOf)	DOI (出版社版) https://doi.org/10.1016/j.clim.2024.110196
アクセス権	open access
権利情報	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Clinical Immunology. 2024, 263, p. 110196
DC.title	Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease
DC.creator	Kinoshita, Riku
	Inoue, Naoya
	Iwatani, Yoshinori
	Noguchi, Yusuke
	Hidaka, Yoh
	Watanabe, Mikio
DC.publisher	Academic Press Inc.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2024-06-01
DC.identifier	https://doi.org/10.1016/j.clim.2024.110196
DC.subject	Autoimmune thyroid disease
	IL-10
	Intractability
	Methylation
	Polymorphism
	Pyrosequencing
	Severity
DC.description	Kinoshita R., Inoue N., Iwatani Y., et al. Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease. Clinical Immunology 263, 110196 (2024); https://doi.org/10.1016/j.clim.2024.110196.
DCTERMS.abstract	The prognosis of autoimmune thyroid diseases (AITDs), including Hashimoto's disease (HD) and Graves' disease (GD), is difficult to predict. DNA methylation regulates gene expression of immune mediating factors. Interleukin (IL)-10 is a Th2 cytokine that downregulates inflammatory cytokines produced by Th1 cells. To clarify the role of methylation of the IL10 gene in the prognosis of AITD, we evaluated the methylation levels of two CpG sites in the IL10 promoter using pyrosequencing. The methylation levels of the −185 CpG site of the IL10 gene were related to age and GD intractability in GD patients. Furthermore, the C carrier of the IL10–592 A/C polymorphism was related to low methylation levels of the −185 CpG site. The methylation levels of the IL10–185 CpG site of the IL10 gene were related to the intractability of GD and were lower in individuals with the C allele of the IL10–592 A/C polymorphism.
DC.rights	© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/
DC.format	95449
citation_title	Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease
citation_author	Kinoshita, Riku
	Inoue, Naoya
	Iwatani, Yoshinori
	Noguchi, Yusuke
	Hidaka, Yoh
	Watanabe, Mikio
citation_publisher	Academic Press Inc.
citation_language	英語
citation_date	2024-06-01
citation_journal_title	Clinical Immunology
citation_volume	263
citation_firstpage	110196
citation_doi	https://doi.org/10.1016/j.clim.2024.110196
citation_public_url	https://hdl.handle.net/11094/95449

Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease

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