Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids

Ondevilla, Joan Candice; Hanashima, Shinya; Mukogawa, Akane; Miyazato, Darcy Garza; Umegawa, Yuichi; Murata, Michio

doi:https://doi.org/10.1016/j.bbamem.2023.184145

Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids

Ondevilla, Joan Candice; Hanashima, Shinya; Mukogawa, Akane et al.

Biochimica et Biophysica Acta - Biomembranes, 2023, 1865(5), 184145

アクセス数:545件（2025-07-16 17:24 集計）

固定URL: https://hdl.handle.net/11094/95489

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タイトル	Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids
著者	著者名 Ondevilla, Joan Candice
	NRID 1000050373353 1000050373353 著者名 Hanashima, Shinya
	著者名 Mukogawa, Akane
	著者名 Miyazato, Darcy Garza
	NRID 1000020587779 1000020587779 ORCID 0000-0002-1057-8119 0000-0002-1057-8119 researchmap permalink yuichi_umegawa yuichi_umegawa Scopus Author ID 8572555200 8572555200 著者名 Umegawa, Yuichi
	NRID 1000040183652 1000040183652 ORCID 0000-0002-1600-145X 0000-0002-1600-145X researchmap permalink quay quay Scopus Author ID 7402129778 7402129778 著者名 Murata, Michio
キーワード等	Dioscin
	Diosgenin
	Lipid bilayers
	Saponin
	Solid-state NMR
	Trillin
内容	Ondevilla J.C., Hanashima S., Mukogawa A., et al. Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids. Biochimica et Biophysica Acta - Biomembranes 1865, 184145 (2023); https://doi.org/10.1016/j.bbamem.2023.184145.
抄録	Saponin is the main bioactive component of the Dioscorea species, which are traditionally used for treating chronic diseases. An understanding of the interaction process of bioactive saponins with biomembranes provides insights into their development as therapeutic agents. The biological effects of saponins have been thought to be associated with membrane cholesterol (Chol). To shed light on the exact mechanisms of their interactions, we investigated the effects of diosgenyl saponins trillin (TRL) and dioscin (DSN) on the dynamic behavior of lipids and membrane properties in palmitoyloleolylphosphatidylcholine (POPC) bilayers using solid-state NMR and fluorescence spectroscopy. The membrane effects of diosgenin, a sapogenin of TRL and DSN, are similar to those of Chol, suggesting that diosgenin plays a major role in membrane binding and POPC chain ordering. The amphiphilicity of TRL and DSN enabled them to interact with POPC bilayers, regardless of Chol. In the presence of Chol, the sugar residues more prominently influenced the membrane-disrupting effects of saponins. The activity of DSN, which bears three sugar units, led to perturbation and further disruption of the membrane in the presence of Chol. However, TRL, which bears one sugar residue, increased the ordering of POPC chains while maintaining the integrity of the bilayer. This effect on the phospholipid bilayers is similar to that of cholesteryl glucoside. The influence of the number of sugars in saponin is discussed in more detail.
出版者	Elsevier B.V.
収録物名	Biochimica et Biophysica Acta - Biomembranes
巻(号)	1865(5)
ページ	184145
刊行年月	2023-06-01
言語	英語
ハンドルURL	https://hdl.handle.net/11094/95489
PISSN	00052736
eISSN	18792642
関連情報 (isIdenticalTo)	DOI (出版社版) https://doi.org/10.1016/j.bbamem.2023.184145
アクセス権	open access
権利情報	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
出版タイプ	Accepted Manuscript
カテゴリ	学術雑誌論文 Journal Article

資源タイプ	学術雑誌論文
ローカル資源タイプ	学術雑誌論文
DCMI資源タイプ	text
DCTERMS.bibliographicCitation	Biochimica et Biophysica Acta - Biomembranes. 2023, 1865(5), p. 184145
DC.title	Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids
DC.creator	Ondevilla, Joan Candice
	Hanashima, Shinya
	Mukogawa, Akane
	Miyazato, Darcy Garza
	Umegawa, Yuichi
	Murata, Michio
DC.publisher	Elsevier B.V.
DC.language' scheme='DCTERMS.RFC1766	英語
DC.type' scheme='DCTERMS.DCMIType	text
DCTERMS.issued' scheme='DCTERMS.W3CDTF	2023-06-01
DC.identifier	https://doi.org/10.1016/j.bbamem.2023.184145
DC.subject	Dioscin
	Diosgenin
	Lipid bilayers
	Saponin
	Solid-state NMR
	Trillin
DC.description	Ondevilla J.C., Hanashima S., Mukogawa A., et al. Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids. Biochimica et Biophysica Acta - Biomembranes 1865, 184145 (2023); https://doi.org/10.1016/j.bbamem.2023.184145.
DCTERMS.abstract	Saponin is the main bioactive component of the Dioscorea species, which are traditionally used for treating chronic diseases. An understanding of the interaction process of bioactive saponins with biomembranes provides insights into their development as therapeutic agents. The biological effects of saponins have been thought to be associated with membrane cholesterol (Chol). To shed light on the exact mechanisms of their interactions, we investigated the effects of diosgenyl saponins trillin (TRL) and dioscin (DSN) on the dynamic behavior of lipids and membrane properties in palmitoyloleolylphosphatidylcholine (POPC) bilayers using solid-state NMR and fluorescence spectroscopy. The membrane effects of diosgenin, a sapogenin of TRL and DSN, are similar to those of Chol, suggesting that diosgenin plays a major role in membrane binding and POPC chain ordering. The amphiphilicity of TRL and DSN enabled them to interact with POPC bilayers, regardless of Chol. In the presence of Chol, the sugar residues more prominently influenced the membrane-disrupting effects of saponins. The activity of DSN, which bears three sugar units, led to perturbation and further disruption of the membrane in the presence of Chol. However, TRL, which bears one sugar residue, increased the ordering of POPC chains while maintaining the integrity of the bilayer. This effect on the phospholipid bilayers is similar to that of cholesteryl glucoside. The influence of the number of sugars in saponin is discussed in more detail.
DC.rights	This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
DC.format	95489
citation_title	Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids
citation_author	Ondevilla, Joan Candice
	Hanashima, Shinya
	Mukogawa, Akane
	Miyazato, Darcy Garza
	Umegawa, Yuichi
	Murata, Michio
citation_publisher	Elsevier B.V.
citation_language	英語
citation_date	2023-06-01
citation_journal_title	Biochimica et Biophysica Acta - Biomembranes
citation_volume	1865
citation_issue	5
citation_firstpage	184145
citation_doi	https://doi.org/10.1016/j.bbamem.2023.184145
citation_public_url	https://hdl.handle.net/11094/95489

Effect of the number of sugar units on the interaction between diosgenyl saponin and membrane lipids

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